Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3934 and GFF3935 overlap by 4 nucleotides GFF3935 and GFF3936 are separated by 9 nucleotides GFF3936 and GFF3937 are separated by 3 nucleotides GFF3937 and GFF3938 overlap by 4 nucleotides
GFF3934 - CoA-acylating propionaldehyde dehydrogenase, at 217,238 to 218,632
GFF3934
GFF3935 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 218,629 to 219,639
GFF3935
GFF3936 - Propanediol utilization polyhedral body protein PduN, at 219,649 to 219,924
GFF3936
GFF3937 - Propanediol utilization protein PduM, at 219,928 to 220,419
GFF3937
GFF3938 - Ethanolamine utilization protein similar to PduL, at 220,416 to 221,048
GFF3938
Position (kb) 218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2 at 217.858 kb on + strand, within GFF3934 at 217.992 kb on - strand, within GFF3934 at 218.158 kb on + strand, within GFF3934 at 218.159 kb on - strand, within GFF3934 at 218.186 kb on - strand, within GFF3934 at 218.312 kb on + strand, within GFF3934 at 218.446 kb on + strand, within GFF3934 at 218.466 kb on - strand, within GFF3934 at 218.487 kb on - strand, within GFF3934 at 218.580 kb on - strand at 218.726 kb on - strand at 219.100 kb on - strand, within GFF3935 at 219.184 kb on - strand, within GFF3935 at 219.205 kb on + strand, within GFF3935 at 219.309 kb on + strand, within GFF3935 at 219.390 kb on - strand, within GFF3935 at 219.627 kb on + strand at 219.663 kb on + strand at 219.667 kb on + strand at 219.875 kb on - strand, within GFF3936 at 219.940 kb on - strand at 219.940 kb on - strand at 220.628 kb on + strand, within GFF3938 at 220.628 kb on + strand, within GFF3938 at 220.629 kb on - strand, within GFF3938
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 217,858 + GFF3934 0.44 -2.1 217,992 - GFF3934 0.54 +0.1 218,158 + GFF3934 0.66 -0.4 218,159 - GFF3934 0.66 -0.1 218,186 - GFF3934 0.68 -0.6 218,312 + GFF3934 0.77 +0.1 218,446 + GFF3934 0.87 +0.3 218,466 - GFF3934 0.88 -0.0 218,487 - GFF3934 0.90 +2.6 218,580 - -0.9 218,726 - -1.7 219,100 - GFF3935 0.47 +1.0 219,184 - GFF3935 0.55 +0.9 219,205 + GFF3935 0.57 +0.5 219,309 + GFF3935 0.67 -0.4 219,390 - GFF3935 0.75 +0.1 219,627 + -0.5 219,663 + -1.6 219,667 + -0.2 219,875 - GFF3936 0.82 +0.6 219,940 - +0.9 219,940 - -0.1 220,628 + GFF3938 0.33 -0.9 220,628 + GFF3938 0.33 +0.6 220,629 - GFF3938 0.34 +1.1
Or see this region's nucleotide sequence