Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3547 and GFF3548 are separated by 153 nucleotides GFF3548 and GFF3549 are separated by 44 nucleotides GFF3549 and GFF3550 are separated by 66 nucleotides
GFF3547 - Coenzyme A transferase, at 299,921 to 301,516
GFF3547
GFF3548 - 3-dehydroquinate dehydratase I (EC 4.2.1.10), at 301,670 to 302,428
GFF3548
GFF3549 - Shikimate/quinate 5-dehydrogenase I beta (EC 1.1.1.282), at 302,473 to 303,339
GFF3549
GFF3550 - Putative transport system permease protein, at 303,406 to 304,641
GFF3550
Position (kb) 302
303
304 Strain fitness (log2 ratio) -2
-1
0
1
2 at 301.686 kb on - strand at 301.828 kb on + strand, within GFF3548 at 301.898 kb on - strand, within GFF3548 at 301.928 kb on - strand, within GFF3548 at 302.040 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.139 kb on + strand, within GFF3548 at 302.195 kb on + strand, within GFF3548 at 302.196 kb on - strand, within GFF3548 at 302.246 kb on + strand, within GFF3548 at 302.434 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand at 302.505 kb on - strand at 302.745 kb on + strand, within GFF3549 at 302.907 kb on + strand, within GFF3549 at 303.030 kb on - strand, within GFF3549 at 303.225 kb on - strand, within GFF3549 at 303.342 kb on + strand at 303.354 kb on + strand at 303.552 kb on + strand, within GFF3550 at 303.744 kb on - strand, within GFF3550 at 303.872 kb on + strand, within GFF3550 at 303.872 kb on + strand, within GFF3550 at 303.927 kb on - strand, within GFF3550 at 304.020 kb on + strand, within GFF3550 at 304.321 kb on + strand, within GFF3550 at 304.324 kb on + strand, within GFF3550
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 301,686 - +0.2 301,828 + GFF3548 0.21 +0.1 301,898 - GFF3548 0.30 -0.5 301,928 - GFF3548 0.34 +0.1 302,040 + GFF3548 0.49 -0.5 302,139 + GFF3548 0.62 -0.3 302,139 + GFF3548 0.62 -1.5 302,139 + GFF3548 0.62 -2.1 302,195 + GFF3548 0.69 +1.3 302,196 - GFF3548 0.69 +0.2 302,246 + GFF3548 0.76 +2.1 302,434 - +0.2 302,505 - -2.0 302,505 - +0.0 302,505 - -0.1 302,745 + GFF3549 0.31 +1.5 302,907 + GFF3549 0.50 -0.3 303,030 - GFF3549 0.64 +0.3 303,225 - GFF3549 0.87 +1.8 303,342 + -1.6 303,354 + +0.7 303,552 + GFF3550 0.12 -0.2 303,744 - GFF3550 0.27 +0.5 303,872 + GFF3550 0.38 -0.8 303,872 + GFF3550 0.38 -1.8 303,927 - GFF3550 0.42 -1.6 304,020 + GFF3550 0.50 -0.4 304,321 + GFF3550 0.74 -0.7 304,324 + GFF3550 0.74 -2.2
Or see this region's nucleotide sequence