Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3321 and GFF3322 are separated by 41 nucleotides GFF3322 and GFF3323 are separated by 61 nucleotides GFF3323 and GFF3324 are separated by 130 nucleotides GFF3324 and GFF3325 are separated by 88 nucleotides
GFF3321 - Minor curlin subunit CsgB, nucleation component of curlin monomers, at 93,184 to 93,639
GFF3321
GFF3322 - Major curlin subunit precursor CsgA, at 93,681 to 94,136
GFF3322
GFF3323 - Putative curli production protein CsgC, at 94,198 to 94,524
GFF3323
GFF3324 - putative periplasmic protein, at 94,655 to 94,975
GFF3324
GFF3325 - COG2110, Macro domain, possibly ADP-ribose binding module, at 95,064 to 95,603
GFF3325
Position (kb) 94
95 Strain fitness (log2 ratio) -2
-1
0
1 at 93.508 kb on + strand, within GFF3321 at 93.612 kb on + strand at 93.613 kb on - strand at 93.638 kb on - strand at 93.718 kb on + strand at 93.945 kb on - strand, within GFF3322 at 93.945 kb on - strand, within GFF3322 at 94.132 kb on - strand at 94.396 kb on - strand, within GFF3323 at 94.464 kb on + strand, within GFF3323 at 94.697 kb on - strand, within GFF3324 at 94.961 kb on - strand at 94.988 kb on - strand at 95.087 kb on - strand at 95.096 kb on + strand at 95.297 kb on + strand, within GFF3325 at 95.439 kb on + strand, within GFF3325 at 95.506 kb on - strand, within GFF3325 at 95.524 kb on + strand, within GFF3325
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 93,508 + GFF3321 0.71 -0.0 93,612 + +0.4 93,613 - +0.2 93,638 - -0.1 93,718 + -0.5 93,945 - GFF3322 0.58 -0.4 93,945 - GFF3322 0.58 -0.3 94,132 - +1.5 94,396 - GFF3323 0.61 -1.8 94,464 + GFF3323 0.81 -0.9 94,697 - GFF3324 0.13 +0.5 94,961 - -1.6 94,988 - -1.5 95,087 - -0.9 95,096 + +0.6 95,297 + GFF3325 0.43 +0.5 95,439 + GFF3325 0.69 -0.4 95,506 - GFF3325 0.82 +1.6 95,524 + GFF3325 0.85 +0.5
Or see this region's nucleotide sequence