Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3279

Experiment: phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3278 and GFF3279 overlap by 4 nucleotidesGFF3279 and GFF3280 are separated by 1 nucleotidesGFF3280 and GFF3281 are separated by 9 nucleotides GFF3278 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 50,087 to 51,376 GFF3278 GFF3279 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 51,373 to 52,839 GFF3279 GFF3280 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 52,841 to 53,692 GFF3280 GFF3281 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 53,702 to 54,082 GFF3281 Position (kb) 51 52 53Strain fitness (log2 ratio) -2 -1 0 1at 50.566 kb on - strand, within GFF3278at 50.780 kb on + strand, within GFF3278at 50.810 kb on - strand, within GFF3278at 50.832 kb on + strand, within GFF3278at 50.901 kb on - strand, within GFF3278at 50.901 kb on - strand, within GFF3278at 50.901 kb on - strand, within GFF3278at 50.909 kb on + strand, within GFF3278at 50.978 kb on + strand, within GFF3278at 50.979 kb on - strand, within GFF3278at 51.085 kb on - strand, within GFF3278at 51.397 kb on - strandat 51.740 kb on - strand, within GFF3279at 51.768 kb on + strand, within GFF3279at 52.066 kb on - strand, within GFF3279at 52.244 kb on - strand, within GFF3279at 52.368 kb on + strand, within GFF3279at 52.369 kb on - strand, within GFF3279at 52.611 kb on + strand, within GFF3279at 52.759 kb on + strandat 52.762 kb on - strandat 53.038 kb on + strand, within GFF3280at 53.039 kb on - strand, within GFF3280at 53.069 kb on - strand, within GFF3280at 53.108 kb on + strand, within GFF3280at 53.115 kb on - strand, within GFF3280at 53.238 kb on - strand, within GFF3280at 53.351 kb on + strand, within GFF3280at 53.352 kb on - strand, within GFF3280at 53.607 kb on - strandat 53.713 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI
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50,566 - GFF3278 0.37 -1.2
50,780 + GFF3278 0.54 +0.3
50,810 - GFF3278 0.56 -0.3
50,832 + GFF3278 0.58 -0.1
50,901 - GFF3278 0.63 -0.1
50,901 - GFF3278 0.63 +0.1
50,901 - GFF3278 0.63 +0.2
50,909 + GFF3278 0.64 -0.1
50,978 + GFF3278 0.69 -0.1
50,979 - GFF3278 0.69 -0.6
51,085 - GFF3278 0.77 -0.4
51,397 - -0.4
51,740 - GFF3279 0.25 -0.4
51,768 + GFF3279 0.27 +0.8
52,066 - GFF3279 0.47 +0.3
52,244 - GFF3279 0.59 +0.7
52,368 + GFF3279 0.68 +0.1
52,369 - GFF3279 0.68 -0.1
52,611 + GFF3279 0.84 +0.7
52,759 + +0.5
52,762 - +0.2
53,038 + GFF3280 0.23 -0.7
53,039 - GFF3280 0.23 +1.5
53,069 - GFF3280 0.27 -1.2
53,108 + GFF3280 0.31 -1.8
53,115 - GFF3280 0.32 +0.4
53,238 - GFF3280 0.47 -0.0
53,351 + GFF3280 0.60 +0.8
53,352 - GFF3280 0.60 -0.3
53,607 - -0.8
53,713 + +0.0

Or see this region's nucleotide sequence