Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3125 and GFF3126 are separated by 165 nucleotides GFF3126 and GFF3127 are separated by 44 nucleotides GFF3127 and GFF3128 are separated by 157 nucleotides
GFF3125 - Methylglyoxal reductase, acetol producing (EC 1.1.1.-) / 2,5-diketo-D-gluconate reductase A (EC 1.1.1.274), at 130,541 to 131,368
GFF3125
GFF3126 - 2-oxoglutarate/malate translocator, at 131,534 to 133,018
GFF3126
GFF3127 - FIG01046325: hypothetical protein, at 133,063 to 133,518
GFF3127
GFF3128 - hypothetical protein, at 133,676 to 133,870
GFF3128
Position (kb) 131
132
133
134 Strain fitness (log2 ratio) -1
0
1 at 130.581 kb on + strand at 130.582 kb on - strand at 130.839 kb on - strand, within GFF3125 at 130.839 kb on - strand, within GFF3125 at 130.936 kb on - strand, within GFF3125 at 130.954 kb on + strand, within GFF3125 at 131.055 kb on + strand, within GFF3125 at 131.128 kb on - strand, within GFF3125 at 131.451 kb on + strand at 131.459 kb on + strand at 131.546 kb on + strand at 131.734 kb on + strand, within GFF3126 at 131.752 kb on + strand, within GFF3126 at 131.781 kb on - strand, within GFF3126 at 131.816 kb on - strand, within GFF3126 at 131.891 kb on - strand, within GFF3126 at 131.940 kb on + strand, within GFF3126 at 132.303 kb on - strand, within GFF3126 at 132.332 kb on - strand, within GFF3126 at 132.714 kb on - strand, within GFF3126 at 132.869 kb on + strand, within GFF3126 at 132.869 kb on + strand, within GFF3126 at 132.912 kb on + strand at 132.913 kb on - strand at 132.913 kb on - strand at 133.014 kb on - strand at 133.207 kb on + strand, within GFF3127 at 133.334 kb on + strand, within GFF3127 at 133.548 kb on + strand at 133.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 130,581 + -0.2 130,582 - -1.3 130,839 - GFF3125 0.36 +1.0 130,839 - GFF3125 0.36 +1.0 130,936 - GFF3125 0.48 -0.9 130,954 + GFF3125 0.50 +0.4 131,055 + GFF3125 0.62 +1.2 131,128 - GFF3125 0.71 +0.7 131,451 + -0.4 131,459 + +0.2 131,546 + -0.1 131,734 + GFF3126 0.13 +0.0 131,752 + GFF3126 0.15 +0.2 131,781 - GFF3126 0.17 +0.3 131,816 - GFF3126 0.19 -0.6 131,891 - GFF3126 0.24 -0.1 131,940 + GFF3126 0.27 +0.6 132,303 - GFF3126 0.52 +0.8 132,332 - GFF3126 0.54 -1.1 132,714 - GFF3126 0.79 +0.3 132,869 + GFF3126 0.90 +1.0 132,869 + GFF3126 0.90 +1.0 132,912 + +0.2 132,913 - -0.1 132,913 - +0.6 133,014 - -0.8 133,207 + GFF3127 0.32 -0.8 133,334 + GFF3127 0.59 +0.3 133,548 + +0.2 133,617 - +0.9
Or see this region's nucleotide sequence