Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3098 and GFF3099 are separated by 67 nucleotides GFF3099 and GFF3100 overlap by 1 nucleotides GFF3100 and GFF3101 are separated by 75 nucleotides GFF3101 and GFF3102 are separated by 27 nucleotides GFF3102 and GFF3103 overlap by 8 nucleotides
GFF3098 - Uncharacterized GST-like protein yghU associated with glutathionylspermidine synthetase/amidase, at 109,565 to 110,431
GFF3098
GFF3099 - Molybdenum-binding periplasmic protein, at 110,499 to 111,209
GFF3099
GFF3100 - Possible ABC-transport protein, periplasmic-binding component, at 111,209 to 112,261
GFF3100
GFF3101 - [NiFe] hydrogenase metallocenter assembly protein HybG, at 112,337 to 112,585
GFF3101
GFF3102 - [NiFe] hydrogenase nickel incorporation protein HybF, at 112,613 to 112,954
GFF3102
GFF3103 - Hydrogenase-2 operon protein hybE, at 112,947 to 113,435
GFF3103
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-2
-1
0
1 at 110.237 kb on + strand, within GFF3098 at 110.237 kb on + strand, within GFF3098 at 110.271 kb on - strand, within GFF3098 at 110.280 kb on + strand, within GFF3098 at 110.281 kb on - strand, within GFF3098 at 110.451 kb on + strand at 110.454 kb on + strand at 110.454 kb on + strand at 110.475 kb on + strand at 110.702 kb on - strand, within GFF3099 at 110.929 kb on - strand, within GFF3099 at 111.109 kb on - strand, within GFF3099 at 111.136 kb on - strand, within GFF3099 at 111.211 kb on - strand at 111.216 kb on - strand at 111.216 kb on - strand at 111.225 kb on + strand at 111.233 kb on - strand at 111.249 kb on + strand at 111.592 kb on - strand, within GFF3100 at 111.634 kb on - strand, within GFF3100 at 111.786 kb on + strand, within GFF3100 at 111.786 kb on + strand, within GFF3100 at 111.787 kb on - strand, within GFF3100 at 112.037 kb on + strand, within GFF3100 at 112.037 kb on + strand, within GFF3100 at 112.095 kb on - strand, within GFF3100 at 112.205 kb on + strand at 112.315 kb on + strand at 112.317 kb on + strand at 112.379 kb on + strand, within GFF3101 at 112.379 kb on + strand, within GFF3101 at 112.433 kb on + strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.434 kb on - strand, within GFF3101 at 112.489 kb on + strand, within GFF3101 at 112.541 kb on - strand, within GFF3101 at 112.579 kb on + strand at 112.586 kb on - strand at 112.628 kb on - strand at 112.857 kb on - strand, within GFF3102 at 112.869 kb on - strand, within GFF3102 at 112.915 kb on + strand, within GFF3102 at 113.031 kb on + strand, within GFF3103
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 110,237 + GFF3098 0.78 +0.2 110,237 + GFF3098 0.78 +0.6 110,271 - GFF3098 0.81 -0.6 110,280 + GFF3098 0.82 +0.3 110,281 - GFF3098 0.83 -0.1 110,451 + -1.0 110,454 + -0.6 110,454 + -1.0 110,475 + +0.4 110,702 - GFF3099 0.29 -0.1 110,929 - GFF3099 0.60 +0.2 111,109 - GFF3099 0.86 -0.7 111,136 - GFF3099 0.90 +0.3 111,211 - +0.3 111,216 - -0.3 111,216 - +0.9 111,225 + -0.8 111,233 - -0.1 111,249 + -0.4 111,592 - GFF3100 0.36 -0.2 111,634 - GFF3100 0.40 -0.2 111,786 + GFF3100 0.55 +0.6 111,786 + GFF3100 0.55 +0.7 111,787 - GFF3100 0.55 -0.1 112,037 + GFF3100 0.79 +0.6 112,037 + GFF3100 0.79 -0.8 112,095 - GFF3100 0.84 +0.3 112,205 + -0.1 112,315 + -0.0 112,317 + +0.3 112,379 + GFF3101 0.17 -0.6 112,379 + GFF3101 0.17 -0.9 112,433 + GFF3101 0.39 +0.1 112,434 - GFF3101 0.39 +0.8 112,434 - GFF3101 0.39 -1.7 112,489 + GFF3101 0.61 -0.1 112,541 - GFF3101 0.82 -0.3 112,579 + +0.2 112,586 - +0.2 112,628 - +0.6 112,857 - GFF3102 0.71 +0.5 112,869 - GFF3102 0.75 +0.6 112,915 + GFF3102 0.88 -0.1 113,031 + GFF3103 0.17 -2.3
Or see this region's nucleotide sequence