Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3100

Experiment: phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3098 and GFF3099 are separated by 67 nucleotidesGFF3099 and GFF3100 overlap by 1 nucleotidesGFF3100 and GFF3101 are separated by 75 nucleotidesGFF3101 and GFF3102 are separated by 27 nucleotidesGFF3102 and GFF3103 overlap by 8 nucleotides GFF3098 - Uncharacterized GST-like protein yghU associated with glutathionylspermidine synthetase/amidase, at 109,565 to 110,431 GFF3098 GFF3099 - Molybdenum-binding periplasmic protein, at 110,499 to 111,209 GFF3099 GFF3100 - Possible ABC-transport protein, periplasmic-binding component, at 111,209 to 112,261 GFF3100 GFF3101 - [NiFe] hydrogenase metallocenter assembly protein HybG, at 112,337 to 112,585 GFF3101 GFF3102 - [NiFe] hydrogenase nickel incorporation protein HybF, at 112,613 to 112,954 GFF3102 GFF3103 - Hydrogenase-2 operon protein hybE, at 112,947 to 113,435 GFF3103 Position (kb) 111 112 113Strain fitness (log2 ratio) -2 -1 0 1at 110.237 kb on + strand, within GFF3098at 110.237 kb on + strand, within GFF3098at 110.271 kb on - strand, within GFF3098at 110.280 kb on + strand, within GFF3098at 110.281 kb on - strand, within GFF3098at 110.451 kb on + strandat 110.454 kb on + strandat 110.454 kb on + strandat 110.475 kb on + strandat 110.702 kb on - strand, within GFF3099at 110.929 kb on - strand, within GFF3099at 111.109 kb on - strand, within GFF3099at 111.136 kb on - strand, within GFF3099at 111.211 kb on - strandat 111.216 kb on - strandat 111.216 kb on - strandat 111.225 kb on + strandat 111.233 kb on - strandat 111.249 kb on + strandat 111.592 kb on - strand, within GFF3100at 111.634 kb on - strand, within GFF3100at 111.786 kb on + strand, within GFF3100at 111.786 kb on + strand, within GFF3100at 111.787 kb on - strand, within GFF3100at 112.037 kb on + strand, within GFF3100at 112.037 kb on + strand, within GFF3100at 112.095 kb on - strand, within GFF3100at 112.205 kb on + strandat 112.315 kb on + strandat 112.317 kb on + strandat 112.379 kb on + strand, within GFF3101at 112.379 kb on + strand, within GFF3101at 112.433 kb on + strand, within GFF3101at 112.434 kb on - strand, within GFF3101at 112.434 kb on - strand, within GFF3101at 112.489 kb on + strand, within GFF3101at 112.541 kb on - strand, within GFF3101at 112.579 kb on + strandat 112.586 kb on - strandat 112.628 kb on - strandat 112.857 kb on - strand, within GFF3102at 112.869 kb on - strand, within GFF3102at 112.915 kb on + strand, within GFF3102at 113.031 kb on + strand, within GFF3103

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Per-strain Table

Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI
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110,237 + GFF3098 0.78 +0.2
110,237 + GFF3098 0.78 +0.6
110,271 - GFF3098 0.81 -0.6
110,280 + GFF3098 0.82 +0.3
110,281 - GFF3098 0.83 -0.1
110,451 + -1.0
110,454 + -0.6
110,454 + -1.0
110,475 + +0.4
110,702 - GFF3099 0.29 -0.1
110,929 - GFF3099 0.60 +0.2
111,109 - GFF3099 0.86 -0.7
111,136 - GFF3099 0.90 +0.3
111,211 - +0.3
111,216 - -0.3
111,216 - +0.9
111,225 + -0.8
111,233 - -0.1
111,249 + -0.4
111,592 - GFF3100 0.36 -0.2
111,634 - GFF3100 0.40 -0.2
111,786 + GFF3100 0.55 +0.6
111,786 + GFF3100 0.55 +0.7
111,787 - GFF3100 0.55 -0.1
112,037 + GFF3100 0.79 +0.6
112,037 + GFF3100 0.79 -0.8
112,095 - GFF3100 0.84 +0.3
112,205 + -0.1
112,315 + -0.0
112,317 + +0.3
112,379 + GFF3101 0.17 -0.6
112,379 + GFF3101 0.17 -0.9
112,433 + GFF3101 0.39 +0.1
112,434 - GFF3101 0.39 +0.8
112,434 - GFF3101 0.39 -1.7
112,489 + GFF3101 0.61 -0.1
112,541 - GFF3101 0.82 -0.3
112,579 + +0.2
112,586 - +0.2
112,628 - +0.6
112,857 - GFF3102 0.71 +0.5
112,869 - GFF3102 0.75 +0.6
112,915 + GFF3102 0.88 -0.1
113,031 + GFF3103 0.17 -2.3

Or see this region's nucleotide sequence