Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2797 and GFF2798 are separated by 62 nucleotides GFF2798 and GFF2799 are separated by 168 nucleotides GFF2799 and GFF2800 are separated by 13 nucleotides GFF2800 and GFF2801 are separated by 170 nucleotides
GFF2797 - Trehalase (EC 3.2.1.28); Periplasmic trehalase precursor (EC 3.2.1.28), at 320,339 to 322,051
GFF2797
GFF2798 - UPF0410 protein YmgE, at 322,114 to 322,368
GFF2798
GFF2799 - Inner membrane protein, at 322,537 to 323,271
GFF2799
GFF2800 - Membrane-bound lytic murein transglycosylase E (EC 3.2.1.-), at 323,285 to 323,896
GFF2800
GFF2801 - Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13), at 324,067 to 324,981
GFF2801
Position (kb)
322
323
324 Strain fitness (log2 ratio)
-2
-1
0
1 at 321.591 kb on + strand, within GFF2797 at 321.592 kb on - strand, within GFF2797 at 321.822 kb on - strand, within GFF2797 at 321.984 kb on + strand at 322.060 kb on + strand at 322.270 kb on - strand, within GFF2798 at 322.399 kb on + strand at 322.400 kb on - strand at 322.405 kb on + strand at 322.511 kb on - strand at 322.547 kb on - strand at 322.586 kb on + strand at 322.755 kb on + strand, within GFF2799 at 322.883 kb on - strand, within GFF2799 at 322.915 kb on + strand, within GFF2799 at 322.952 kb on + strand, within GFF2799 at 323.024 kb on + strand, within GFF2799 at 323.025 kb on - strand, within GFF2799 at 323.282 kb on - strand at 323.461 kb on - strand, within GFF2800 at 323.461 kb on - strand, within GFF2800 at 323.861 kb on + strand at 323.862 kb on - strand at 323.865 kb on - strand at 323.865 kb on - strand at 323.904 kb on - strand at 323.922 kb on + strand at 323.923 kb on - strand at 323.960 kb on + strand at 323.961 kb on - strand at 323.961 kb on - strand at 323.961 kb on - strand at 324.014 kb on + strand at 324.017 kb on + strand at 324.034 kb on - strand at 324.047 kb on - strand at 324.047 kb on - strand at 324.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 321,591 + GFF2797 0.73 +0.6 321,592 - GFF2797 0.73 +0.7 321,822 - GFF2797 0.87 +0.4 321,984 + +0.2 322,060 + +0.5 322,270 - GFF2798 0.61 +0.7 322,399 + -0.1 322,400 - -0.5 322,405 + +0.7 322,511 - -1.0 322,547 - +0.1 322,586 + +0.8 322,755 + GFF2799 0.30 +0.4 322,883 - GFF2799 0.47 -0.6 322,915 + GFF2799 0.51 -0.1 322,952 + GFF2799 0.56 +0.7 323,024 + GFF2799 0.66 +1.1 323,025 - GFF2799 0.66 +0.8 323,282 - +0.4 323,461 - GFF2800 0.29 +0.7 323,461 - GFF2800 0.29 -0.1 323,861 + -2.4 323,862 - +0.2 323,865 - +0.2 323,865 - -1.4 323,904 - +0.6 323,922 + -0.7 323,923 - +0.6 323,960 + -0.1 323,961 - +1.3 323,961 - +0.0 323,961 - +0.1 324,014 + -0.3 324,017 + -0.0 324,034 - +0.3 324,047 - -0.3 324,047 - +0.1 324,064 - +1.3
Or see this region's nucleotide sequence