Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2650 and GFF2651 are separated by 145 nucleotides GFF2651 and GFF2652 are separated by 52 nucleotides GFF2652 and GFF2653 are separated by 78 nucleotides GFF2653 and GFF2654 overlap by 20 nucleotides
GFF2650 - Zinc transport protein ZntB, at 175,766 to 176,749
GFF2650
GFF2651 - Putative methyl-accepting chemotaxis protein, at 176,895 to 178,007
GFF2651
GFF2652 - FIG01046553: hypothetical protein, at 178,060 to 178,221
GFF2652
GFF2653 - FIG01045924: hypothetical protein, at 178,300 to 178,479
GFF2653
GFF2654 - FIG00638524: hypothetical protein, at 178,460 to 179,023
GFF2654
Position (kb) 176
177
178
179 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 175.945 kb on + strand, within GFF2650 at 175.947 kb on + strand, within GFF2650 at 176.127 kb on + strand, within GFF2650 at 176.151 kb on + strand, within GFF2650 at 176.180 kb on + strand, within GFF2650 at 176.267 kb on - strand, within GFF2650 at 176.420 kb on - strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.421 kb on + strand, within GFF2650 at 176.422 kb on - strand, within GFF2650 at 176.470 kb on + strand, within GFF2650 at 176.513 kb on + strand, within GFF2650 at 176.608 kb on + strand, within GFF2650 at 176.610 kb on + strand, within GFF2650 at 176.611 kb on - strand, within GFF2650 at 176.615 kb on - strand, within GFF2650 at 176.621 kb on + strand, within GFF2650 at 176.622 kb on - strand, within GFF2650 at 176.656 kb on + strand at 176.656 kb on + strand at 176.934 kb on + strand at 176.934 kb on + strand at 176.935 kb on - strand at 176.935 kb on - strand at 176.935 kb on - strand at 177.027 kb on + strand, within GFF2651 at 177.028 kb on - strand, within GFF2651 at 177.111 kb on + strand, within GFF2651 at 177.228 kb on + strand, within GFF2651 at 177.471 kb on - strand, within GFF2651 at 177.546 kb on + strand, within GFF2651 at 177.603 kb on + strand, within GFF2651 at 177.652 kb on - strand, within GFF2651 at 177.762 kb on - strand, within GFF2651 at 177.809 kb on + strand, within GFF2651 at 177.846 kb on - strand, within GFF2651 at 177.890 kb on + strand, within GFF2651 at 177.892 kb on + strand, within GFF2651 at 177.980 kb on + strand at 178.002 kb on + strand at 178.245 kb on - strand at 178.395 kb on + strand, within GFF2653 at 178.396 kb on - strand, within GFF2653 at 178.468 kb on + strand at 178.516 kb on + strand at 178.531 kb on - strand, within GFF2654 at 178.580 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.614 kb on + strand, within GFF2654 at 178.678 kb on + strand, within GFF2654 at 178.861 kb on + strand, within GFF2654 at 178.888 kb on + strand, within GFF2654 at 178.949 kb on + strand, within GFF2654
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 175,945 + GFF2650 0.18 +0.4 175,947 + GFF2650 0.18 -0.9 176,127 + GFF2650 0.37 +0.4 176,151 + GFF2650 0.39 -2.0 176,180 + GFF2650 0.42 -1.0 176,267 - GFF2650 0.51 -1.9 176,420 - GFF2650 0.66 +1.4 176,421 + GFF2650 0.67 -0.1 176,421 + GFF2650 0.67 -2.0 176,421 + GFF2650 0.67 -0.3 176,422 - GFF2650 0.67 +0.7 176,470 + GFF2650 0.72 -0.1 176,513 + GFF2650 0.76 -1.3 176,608 + GFF2650 0.86 +0.1 176,610 + GFF2650 0.86 -0.1 176,611 - GFF2650 0.86 +0.7 176,615 - GFF2650 0.86 +0.0 176,621 + GFF2650 0.87 +1.0 176,622 - GFF2650 0.87 +0.0 176,656 + -3.1 176,656 + -2.3 176,934 + -1.7 176,934 + +0.2 176,935 - +0.8 176,935 - -0.3 176,935 - +0.5 177,027 + GFF2651 0.12 +0.1 177,028 - GFF2651 0.12 -0.8 177,111 + GFF2651 0.19 +1.0 177,228 + GFF2651 0.30 -0.4 177,471 - GFF2651 0.52 +0.2 177,546 + GFF2651 0.58 +1.1 177,603 + GFF2651 0.64 +3.3 177,652 - GFF2651 0.68 +0.4 177,762 - GFF2651 0.78 +1.1 177,809 + GFF2651 0.82 +0.2 177,846 - GFF2651 0.85 +0.3 177,890 + GFF2651 0.89 -0.6 177,892 + GFF2651 0.90 -1.0 177,980 + -0.7 178,002 + -0.5 178,245 - -1.0 178,395 + GFF2653 0.53 +1.9 178,396 - GFF2653 0.53 +0.5 178,468 + -0.1 178,516 + +0.3 178,531 - GFF2654 0.13 +0.4 178,580 + GFF2654 0.21 -0.8 178,614 + GFF2654 0.27 -0.5 178,614 + GFF2654 0.27 +0.2 178,678 + GFF2654 0.39 +0.1 178,861 + GFF2654 0.71 -0.1 178,888 + GFF2654 0.76 +0.3 178,949 + GFF2654 0.87 +0.1
Or see this region's nucleotide sequence