Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2630 and GFF2631 are separated by 116 nucleotides GFF2631 and GFF2632 are separated by 63 nucleotides GFF2632 and GFF2633 are separated by 200 nucleotides GFF2633 and GFF2634 overlap by 4 nucleotides
GFF2630 - Cytochrome b(561), at 155,441 to 155,971
GFF2630
GFF2631 - Protein ydcF, at 156,088 to 156,888
GFF2631
GFF2632 - ATP-dependent RNA helicase HrpA (EC 3.6.4.13), at 156,952 to 160,854
GFF2632
GFF2633 - FMN-dependent NADH-azoreductase, at 161,055 to 161,660
GFF2633
GFF2634 - Gfa-like protein, at 161,657 to 161,983
GFF2634
Position (kb)
156
157
158
159
160
161 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 156.008 kb on - strand at 156.019 kb on - strand at 156.021 kb on - strand at 156.095 kb on - strand at 156.126 kb on + strand at 156.142 kb on + strand at 156.189 kb on + strand, within GFF2631 at 156.517 kb on - strand, within GFF2631 at 156.676 kb on + strand, within GFF2631 at 156.677 kb on - strand, within GFF2631 at 156.786 kb on - strand, within GFF2631 at 156.882 kb on + strand at 156.999 kb on + strand at 157.413 kb on + strand, within GFF2632 at 157.414 kb on - strand, within GFF2632 at 157.493 kb on - strand, within GFF2632 at 157.500 kb on + strand, within GFF2632 at 157.501 kb on - strand, within GFF2632 at 157.604 kb on + strand, within GFF2632 at 157.605 kb on - strand, within GFF2632 at 158.069 kb on - strand, within GFF2632 at 158.150 kb on - strand, within GFF2632 at 158.150 kb on - strand, within GFF2632 at 158.223 kb on + strand, within GFF2632 at 158.267 kb on + strand, within GFF2632 at 158.433 kb on + strand, within GFF2632 at 158.434 kb on - strand, within GFF2632 at 158.645 kb on + strand, within GFF2632 at 158.681 kb on + strand, within GFF2632 at 158.744 kb on + strand, within GFF2632 at 159.062 kb on + strand, within GFF2632 at 159.063 kb on - strand, within GFF2632 at 159.063 kb on - strand, within GFF2632 at 159.066 kb on + strand, within GFF2632 at 159.093 kb on - strand, within GFF2632 at 159.101 kb on + strand, within GFF2632 at 159.376 kb on + strand, within GFF2632 at 159.377 kb on - strand, within GFF2632 at 159.403 kb on - strand, within GFF2632 at 159.430 kb on - strand, within GFF2632 at 159.471 kb on + strand, within GFF2632 at 159.770 kb on + strand, within GFF2632 at 159.770 kb on + strand, within GFF2632 at 159.771 kb on - strand, within GFF2632 at 159.771 kb on - strand, within GFF2632 at 159.903 kb on + strand, within GFF2632 at 160.030 kb on + strand, within GFF2632 at 160.076 kb on + strand, within GFF2632 at 160.131 kb on + strand, within GFF2632 at 160.132 kb on - strand, within GFF2632 at 160.173 kb on + strand, within GFF2632 at 160.174 kb on - strand, within GFF2632 at 160.176 kb on + strand, within GFF2632 at 160.176 kb on + strand, within GFF2632 at 160.269 kb on + strand, within GFF2632 at 160.294 kb on + strand, within GFF2632 at 160.360 kb on - strand, within GFF2632 at 160.386 kb on + strand, within GFF2632 at 160.389 kb on - strand, within GFF2632 at 160.474 kb on - strand at 160.840 kb on - strand at 160.990 kb on - strand at 160.990 kb on - strand at 161.066 kb on - strand at 161.069 kb on - strand at 161.344 kb on - strand, within GFF2633 at 161.415 kb on + strand, within GFF2633 at 161.521 kb on - strand, within GFF2633 at 161.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 156,008 - +0.5 156,019 - -0.2 156,021 - -0.6 156,095 - -0.4 156,126 + -0.4 156,142 + +0.6 156,189 + GFF2631 0.13 +0.9 156,517 - GFF2631 0.54 +0.6 156,676 + GFF2631 0.73 -2.4 156,677 - GFF2631 0.74 -1.4 156,786 - GFF2631 0.87 -1.6 156,882 + -1.1 156,999 + -0.3 157,413 + GFF2632 0.12 -1.3 157,414 - GFF2632 0.12 -0.7 157,493 - GFF2632 0.14 +0.8 157,500 + GFF2632 0.14 -0.3 157,501 - GFF2632 0.14 +1.8 157,604 + GFF2632 0.17 +1.1 157,605 - GFF2632 0.17 +0.6 158,069 - GFF2632 0.29 +0.8 158,150 - GFF2632 0.31 +1.4 158,150 - GFF2632 0.31 +0.5 158,223 + GFF2632 0.33 +0.6 158,267 + GFF2632 0.34 +0.6 158,433 + GFF2632 0.38 +1.0 158,434 - GFF2632 0.38 +0.1 158,645 + GFF2632 0.43 +0.6 158,681 + GFF2632 0.44 +0.6 158,744 + GFF2632 0.46 -0.6 159,062 + GFF2632 0.54 -0.4 159,063 - GFF2632 0.54 +0.5 159,063 - GFF2632 0.54 -0.0 159,066 + GFF2632 0.54 +0.6 159,093 - GFF2632 0.55 +0.9 159,101 + GFF2632 0.55 +0.1 159,376 + GFF2632 0.62 -1.5 159,377 - GFF2632 0.62 +0.6 159,403 - GFF2632 0.63 +0.5 159,430 - GFF2632 0.63 +0.9 159,471 + GFF2632 0.65 +0.4 159,770 + GFF2632 0.72 +0.7 159,770 + GFF2632 0.72 -0.8 159,771 - GFF2632 0.72 +0.9 159,771 - GFF2632 0.72 -0.8 159,903 + GFF2632 0.76 -0.3 160,030 + GFF2632 0.79 +0.8 160,076 + GFF2632 0.80 +1.1 160,131 + GFF2632 0.81 +0.3 160,132 - GFF2632 0.81 +0.1 160,173 + GFF2632 0.83 +0.1 160,174 - GFF2632 0.83 -1.6 160,176 + GFF2632 0.83 -2.6 160,176 + GFF2632 0.83 +1.0 160,269 + GFF2632 0.85 +0.3 160,294 + GFF2632 0.86 +0.1 160,360 - GFF2632 0.87 -0.2 160,386 + GFF2632 0.88 +0.6 160,389 - GFF2632 0.88 -1.5 160,474 - +0.9 160,840 - +0.6 160,990 - -0.7 160,990 - -0.9 161,066 - +1.1 161,069 - +1.8 161,344 - GFF2633 0.48 -0.0 161,415 + GFF2633 0.59 -0.5 161,521 - GFF2633 0.77 -1.7 161,659 - +0.3
Or see this region's nucleotide sequence