Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2616 and GFF2617 are separated by 186 nucleotides GFF2617 and GFF2618 are separated by 31 nucleotides
GFF2616 - Methyl-accepting chemotaxis protein III (ribose and galactose chemoreceptor protein), at 141,497 to 143,104
GFF2616
GFF2617 - S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284), at 143,291 to 144,409
GFF2617
GFF2618 - FrmR: Negative transcriptional regulator of formaldehyde detoxification operon, at 144,441 to 144,716
GFF2618
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-1
0
1
2 at 142.487 kb on - strand, within GFF2616 at 142.523 kb on - strand, within GFF2616 at 142.547 kb on + strand, within GFF2616 at 142.598 kb on - strand, within GFF2616 at 142.600 kb on - strand, within GFF2616 at 142.699 kb on + strand, within GFF2616 at 142.771 kb on + strand, within GFF2616 at 142.771 kb on + strand, within GFF2616 at 142.772 kb on - strand, within GFF2616 at 142.898 kb on - strand, within GFF2616 at 142.901 kb on + strand, within GFF2616 at 143.148 kb on - strand at 143.575 kb on + strand, within GFF2617 at 143.654 kb on - strand, within GFF2617 at 143.665 kb on + strand, within GFF2617 at 143.923 kb on + strand, within GFF2617 at 143.979 kb on + strand, within GFF2617 at 144.124 kb on + strand, within GFF2617 at 144.147 kb on + strand, within GFF2617 at 144.246 kb on - strand, within GFF2617 at 144.246 kb on - strand, within GFF2617 at 144.275 kb on - strand, within GFF2617 at 144.302 kb on - strand at 144.411 kb on + strand at 144.443 kb on - strand at 144.446 kb on + strand at 144.686 kb on + strand, within GFF2618 at 144.733 kb on - strand at 144.946 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 142,487 - GFF2616 0.62 +0.1 142,523 - GFF2616 0.64 +0.4 142,547 + GFF2616 0.65 -0.3 142,598 - GFF2616 0.68 -0.2 142,600 - GFF2616 0.69 +1.2 142,699 + GFF2616 0.75 -0.0 142,771 + GFF2616 0.79 +0.1 142,771 + GFF2616 0.79 +0.3 142,772 - GFF2616 0.79 -0.1 142,898 - GFF2616 0.87 -1.2 142,901 + GFF2616 0.87 -0.5 143,148 - -0.8 143,575 + GFF2617 0.25 -1.7 143,654 - GFF2617 0.32 +0.4 143,665 + GFF2617 0.33 +1.3 143,923 + GFF2617 0.56 +0.4 143,979 + GFF2617 0.61 +0.2 144,124 + GFF2617 0.74 -1.6 144,147 + GFF2617 0.76 +0.1 144,246 - GFF2617 0.85 +0.8 144,246 - GFF2617 0.85 +0.6 144,275 - GFF2617 0.88 +0.1 144,302 - +0.1 144,411 + -0.6 144,443 - +0.1 144,446 + +0.2 144,686 + GFF2618 0.89 +2.2 144,733 - +0.7 144,946 + -0.4
Or see this region's nucleotide sequence