Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2560 and GFF2561 are separated by 22 nucleotides GFF2561 and GFF2562 are separated by 159 nucleotides
GFF2560 - Formate dehydrogenase N alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 76,059 to 79,106
GFF2560
GFF2561 - hypothetical protein, at 79,129 to 79,281
GFF2561
GFF2562 - Permease of the drug/metabolite transporter (DMT) superfamily, at 79,441 to 80,265
GFF2562
Position (kb)
79
80 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 78.194 kb on - strand, within GFF2560 at 78.194 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.293 kb on - strand, within GFF2560 at 78.446 kb on + strand, within GFF2560 at 78.449 kb on + strand, within GFF2560 at 78.735 kb on - strand, within GFF2560 at 78.783 kb on + strand, within GFF2560 at 78.784 kb on - strand, within GFF2560 at 78.805 kb on + strand at 78.920 kb on + strand at 78.922 kb on + strand at 78.922 kb on + strand at 78.923 kb on - strand at 78.992 kb on + strand at 79.029 kb on - strand at 79.196 kb on + strand, within GFF2561 at 79.254 kb on - strand, within GFF2561 at 79.279 kb on + strand at 79.339 kb on + strand at 79.406 kb on + strand at 79.409 kb on + strand at 79.410 kb on - strand at 79.505 kb on - strand at 79.505 kb on - strand at 79.545 kb on - strand, within GFF2562 at 79.573 kb on + strand, within GFF2562 at 79.686 kb on - strand, within GFF2562 at 79.827 kb on + strand, within GFF2562 at 79.884 kb on + strand, within GFF2562 at 79.919 kb on - strand, within GFF2562 at 79.979 kb on + strand, within GFF2562 at 80.013 kb on - strand, within GFF2562 at 80.093 kb on + strand, within GFF2562 at 80.113 kb on - strand, within GFF2562 at 80.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 78,194 - GFF2560 0.70 -1.6 78,194 - GFF2560 0.70 +0.9 78,293 - GFF2560 0.73 +0.2 78,293 - GFF2560 0.73 +0.3 78,446 + GFF2560 0.78 -2.1 78,449 + GFF2560 0.78 +0.2 78,735 - GFF2560 0.88 -0.2 78,783 + GFF2560 0.89 -0.2 78,784 - GFF2560 0.89 +0.2 78,805 + -0.5 78,920 + +0.2 78,922 + -0.8 78,922 + +0.0 78,923 - +0.3 78,992 + +0.6 79,029 - +0.2 79,196 + GFF2561 0.44 +0.5 79,254 - GFF2561 0.82 +0.9 79,279 + -1.4 79,339 + -0.8 79,406 + -0.3 79,409 + -0.3 79,410 - +0.1 79,505 - +0.5 79,505 - -2.2 79,545 - GFF2562 0.13 -0.9 79,573 + GFF2562 0.16 -0.1 79,686 - GFF2562 0.30 +1.2 79,827 + GFF2562 0.47 +0.1 79,884 + GFF2562 0.54 -0.8 79,919 - GFF2562 0.58 -0.1 79,979 + GFF2562 0.65 -0.6 80,013 - GFF2562 0.69 -0.9 80,093 + GFF2562 0.79 +1.9 80,113 - GFF2562 0.81 -0.8 80,263 + +0.1
Or see this region's nucleotide sequence