Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1516 and GFF1517 are separated by 66 nucleotides GFF1517 and GFF1518 are separated by 9 nucleotides GFF1518 and GFF1519 are separated by 21 nucleotides GFF1519 and GFF1520 overlap by 43 nucleotides
GFF1516 - Nucleoside-diphosphate-sugar epimerases, at 20,937 to 21,572
GFF1516
GFF1517 - 21 kDa hemolysin precursor, at 21,639 to 22,214
GFF1517
GFF1518 - Phosphoheptose isomerase (EC 5.3.1.-), at 22,224 to 22,814
GFF1518
GFF1519 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 22,836 to 23,231
GFF1519
GFF1520 - LppC putative lipoprotein, at 23,189 to 25,231
GFF1520
Position (kb)
22
23 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 21.377 kb on + strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.378 kb on - strand, within GFF1516 at 21.570 kb on + strand at 21.609 kb on - strand at 21.640 kb on + strand at 21.644 kb on + strand at 21.645 kb on - strand at 21.645 kb on - strand at 21.645 kb on - strand at 21.647 kb on + strand at 21.648 kb on - strand at 21.653 kb on + strand at 21.653 kb on + strand at 21.891 kb on - strand, within GFF1517 at 21.892 kb on + strand, within GFF1517 at 21.925 kb on - strand, within GFF1517 at 21.972 kb on + strand, within GFF1517 at 22.170 kb on + strand at 22.170 kb on + strand at 22.226 kb on - strand at 22.226 kb on - strand at 22.290 kb on - strand, within GFF1518 at 22.356 kb on + strand, within GFF1518 at 22.357 kb on - strand, within GFF1518 at 22.558 kb on - strand, within GFF1518 at 22.817 kb on + strand at 22.893 kb on + strand, within GFF1519 at 22.961 kb on + strand, within GFF1519 at 22.962 kb on - strand, within GFF1519 at 22.962 kb on - strand, within GFF1519 at 22.969 kb on - strand, within GFF1519 at 23.364 kb on + strand at 23.394 kb on - strand, within GFF1520 at 23.458 kb on - strand, within GFF1520 at 23.462 kb on + strand, within GFF1520 at 23.463 kb on - strand, within GFF1520 at 23.611 kb on - strand, within GFF1520
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 21,377 + GFF1516 0.69 +0.5 21,378 - GFF1516 0.69 -0.4 21,378 - GFF1516 0.69 -0.2 21,378 - GFF1516 0.69 +0.1 21,570 + +0.5 21,609 - -0.5 21,640 + +0.7 21,644 + -0.2 21,645 - +0.8 21,645 - -0.0 21,645 - -0.3 21,647 + -1.7 21,648 - -0.0 21,653 + +0.6 21,653 + +0.2 21,891 - GFF1517 0.44 -0.1 21,892 + GFF1517 0.44 +0.0 21,925 - GFF1517 0.50 -0.4 21,972 + GFF1517 0.58 -0.3 22,170 + -1.1 22,170 + -1.7 22,226 - -0.8 22,226 - -0.1 22,290 - GFF1518 0.11 -0.6 22,356 + GFF1518 0.22 -0.3 22,357 - GFF1518 0.23 -0.5 22,558 - GFF1518 0.57 -1.1 22,817 + -1.7 22,893 + GFF1519 0.14 -4.0 22,961 + GFF1519 0.32 +1.1 22,962 - GFF1519 0.32 +0.3 22,962 - GFF1519 0.32 +0.2 22,969 - GFF1519 0.34 +0.3 23,364 + -0.7 23,394 - GFF1520 0.10 -0.3 23,458 - GFF1520 0.13 -0.0 23,462 + GFF1520 0.13 -2.3 23,463 - GFF1520 0.13 -0.3 23,611 - GFF1520 0.21 +0.4
Or see this region's nucleotide sequence