Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1350 and GFF1351 are separated by 265 nucleotides GFF1351 and GFF1352 are separated by 31 nucleotides
GFF1350 - Homoserine O-succinyltransferase (EC 2.3.1.46), at 969 to 1,898
GFF1350
GFF1351 - Malate synthase (EC 2.3.3.9), at 2,164 to 3,768
GFF1351
GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104
GFF1352
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.234 kb on - strand, within GFF1350 at 1.374 kb on - strand, within GFF1350 at 1.419 kb on - strand, within GFF1350 at 1.470 kb on + strand, within GFF1350 at 1.470 kb on + strand, within GFF1350 at 1.549 kb on - strand, within GFF1350 at 1.647 kb on - strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.653 kb on - strand, within GFF1350 at 1.680 kb on + strand, within GFF1350 at 1.755 kb on + strand, within GFF1350 at 1.824 kb on - strand at 1.842 kb on + strand at 2.139 kb on - strand at 2.199 kb on - strand at 2.466 kb on + strand, within GFF1351 at 2.467 kb on - strand, within GFF1351 at 2.479 kb on - strand, within GFF1351 at 2.553 kb on + strand, within GFF1351 at 2.602 kb on + strand, within GFF1351 at 2.602 kb on + strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.666 kb on + strand, within GFF1351 at 2.667 kb on - strand, within GFF1351 at 2.676 kb on - strand, within GFF1351 at 2.697 kb on + strand, within GFF1351 at 2.698 kb on - strand, within GFF1351 at 2.762 kb on - strand, within GFF1351 at 2.762 kb on - strand, within GFF1351 at 2.784 kb on + strand, within GFF1351 at 2.800 kb on + strand, within GFF1351 at 3.633 kb on - strand at 3.767 kb on - strand at 3.778 kb on + strand at 3.832 kb on - strand at 4.064 kb on + strand, within GFF1352 at 4.065 kb on - strand, within GFF1352 at 4.116 kb on - strand, within GFF1352 at 4.247 kb on + strand, within GFF1352 at 4.555 kb on + strand, within GFF1352 at 4.586 kb on + strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.673 kb on - strand, within GFF1352 at 4.747 kb on + strand, within GFF1352
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 1,234 - GFF1350 0.28 +0.1 1,374 - GFF1350 0.44 -2.0 1,419 - GFF1350 0.48 -0.1 1,470 + GFF1350 0.54 -0.6 1,470 + GFF1350 0.54 -0.2 1,549 - GFF1350 0.62 +0.0 1,647 - GFF1350 0.73 +0.1 1,652 + GFF1350 0.73 +0.8 1,652 + GFF1350 0.73 -0.2 1,652 + GFF1350 0.73 +0.8 1,653 - GFF1350 0.74 -0.1 1,680 + GFF1350 0.76 -0.1 1,755 + GFF1350 0.85 -0.2 1,824 - -1.3 1,842 + -0.2 2,139 - -0.4 2,199 - +0.2 2,466 + GFF1351 0.19 -0.7 2,467 - GFF1351 0.19 -0.0 2,479 - GFF1351 0.20 -0.3 2,553 + GFF1351 0.24 -0.4 2,602 + GFF1351 0.27 +0.2 2,602 + GFF1351 0.27 -0.4 2,603 - GFF1351 0.27 +0.2 2,603 - GFF1351 0.27 -1.4 2,603 - GFF1351 0.27 -0.1 2,666 + GFF1351 0.31 +0.4 2,667 - GFF1351 0.31 -0.8 2,676 - GFF1351 0.32 +1.5 2,697 + GFF1351 0.33 +0.1 2,698 - GFF1351 0.33 -0.3 2,762 - GFF1351 0.37 -0.2 2,762 - GFF1351 0.37 +0.5 2,784 + GFF1351 0.39 +0.2 2,800 + GFF1351 0.40 -0.1 3,633 - -0.0 3,767 - +0.4 3,778 + -0.5 3,832 - +0.1 4,064 + GFF1352 0.20 +2.4 4,065 - GFF1352 0.20 -0.1 4,116 - GFF1352 0.24 +0.0 4,247 + GFF1352 0.34 +0.1 4,555 + GFF1352 0.58 -0.3 4,586 + GFF1352 0.60 -0.4 4,673 - GFF1352 0.67 -0.3 4,673 - GFF1352 0.67 -0.9 4,673 - GFF1352 0.67 +0.7 4,747 + GFF1352 0.73 +0.3
Or see this region's nucleotide sequence