Experiment: phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1157 and GFF1158 overlap by 14 nucleotides GFF1158 and GFF1159 overlap by 1 nucleotides GFF1159 and GFF1160 overlap by 11 nucleotides GFF1160 and GFF1161 overlap by 14 nucleotides GFF1161 and GFF1162 overlap by 4 nucleotides
GFF1157 - IncF plasmid conjugative transfer pilus assembly protein TraE, at 9,630 to 10,196
GFF1157
GFF1158 - IncF plasmid conjugative transfer pilus assembly protein TraK, at 10,183 to 10,923
GFF1158
GFF1159 - IncF plasmid conjugative transfer pilus assembly protein TraB, at 10,923 to 12,344
GFF1159
GFF1160 - IncF plasmid conjugative transfer protein TraP, at 12,334 to 12,930
GFF1160
GFF1161 - IncF plasmid conjugative transfer protein TrbD, at 12,917 to 13,153
GFF1161
GFF1162 - IncF plasmid conjugative transfer pilus assembly protein TraV, at 13,150 to 13,665
GFF1162
Position (kb)
10
11
12
13 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 9.924 kb on + strand, within GFF1157 at 9.925 kb on - strand, within GFF1157 at 9.925 kb on - strand, within GFF1157 at 10.001 kb on + strand, within GFF1157 at 10.045 kb on - strand, within GFF1157 at 10.120 kb on - strand, within GFF1157 at 10.201 kb on + strand at 10.202 kb on - strand at 10.347 kb on + strand, within GFF1158 at 10.348 kb on - strand, within GFF1158 at 10.539 kb on - strand, within GFF1158 at 10.539 kb on - strand, within GFF1158 at 10.603 kb on + strand, within GFF1158 at 10.894 kb on + strand at 10.894 kb on + strand at 10.894 kb on + strand at 10.895 kb on - strand at 10.895 kb on - strand at 10.937 kb on + strand at 10.959 kb on + strand at 11.017 kb on - strand at 11.017 kb on - strand at 11.208 kb on - strand, within GFF1159 at 11.330 kb on + strand, within GFF1159 at 11.378 kb on + strand, within GFF1159 at 11.378 kb on + strand, within GFF1159 at 11.469 kb on + strand, within GFF1159 at 11.470 kb on - strand, within GFF1159 at 11.470 kb on - strand, within GFF1159 at 11.470 kb on - strand, within GFF1159 at 11.496 kb on - strand, within GFF1159 at 11.496 kb on - strand, within GFF1159 at 11.496 kb on - strand, within GFF1159 at 11.504 kb on + strand, within GFF1159 at 11.504 kb on + strand, within GFF1159 at 11.504 kb on + strand, within GFF1159 at 11.517 kb on + strand, within GFF1159 at 11.553 kb on + strand, within GFF1159 at 11.553 kb on + strand, within GFF1159 at 11.555 kb on + strand, within GFF1159 at 11.684 kb on + strand, within GFF1159 at 11.685 kb on - strand, within GFF1159 at 11.685 kb on - strand, within GFF1159 at 11.692 kb on - strand, within GFF1159 at 11.692 kb on - strand, within GFF1159 at 11.692 kb on - strand, within GFF1159 at 11.758 kb on + strand, within GFF1159 at 11.759 kb on - strand, within GFF1159 at 11.759 kb on - strand, within GFF1159 at 11.759 kb on - strand, within GFF1159 at 11.762 kb on + strand, within GFF1159 at 11.762 kb on + strand, within GFF1159 at 11.768 kb on + strand, within GFF1159 at 11.769 kb on - strand, within GFF1159 at 11.771 kb on + strand, within GFF1159 at 11.771 kb on + strand, within GFF1159 at 11.771 kb on + strand, within GFF1159 at 12.220 kb on + strand at 12.220 kb on + strand at 12.242 kb on + strand at 12.243 kb on - strand at 12.305 kb on + strand at 12.332 kb on - strand at 12.342 kb on + strand at 12.384 kb on + strand at 12.385 kb on - strand at 12.385 kb on - strand at 12.424 kb on + strand, within GFF1160 at 12.659 kb on - strand, within GFF1160 at 12.727 kb on + strand, within GFF1160 at 12.727 kb on + strand, within GFF1160 at 12.744 kb on - strand, within GFF1160 at 12.785 kb on - strand, within GFF1160 at 12.924 kb on + strand at 12.928 kb on + strand at 12.934 kb on + strand at 13.234 kb on - strand, within GFF1162 at 13.257 kb on + strand, within GFF1162 at 13.257 kb on + strand, within GFF1162 at 13.257 kb on + strand, within GFF1162
Per-strain Table
Position Strand Gene LocusTag Fraction phi19h1_phage 15 MOI remove 9,924 + GFF1157 0.52 -0.4 9,925 - GFF1157 0.52 +1.1 9,925 - GFF1157 0.52 -0.2 10,001 + GFF1157 0.65 +0.2 10,045 - GFF1157 0.73 -0.7 10,120 - GFF1157 0.86 -0.3 10,201 + -0.5 10,202 - +0.3 10,347 + GFF1158 0.22 +0.4 10,348 - GFF1158 0.22 +0.0 10,539 - GFF1158 0.48 +0.1 10,539 - GFF1158 0.48 +0.4 10,603 + GFF1158 0.57 -0.2 10,894 + -0.5 10,894 + +0.0 10,894 + +0.9 10,895 - -0.3 10,895 - -1.2 10,937 + +0.4 10,959 + +0.9 11,017 - -0.1 11,017 - -0.7 11,208 - GFF1159 0.20 +0.3 11,330 + GFF1159 0.29 +0.9 11,378 + GFF1159 0.32 -0.2 11,378 + GFF1159 0.32 +0.1 11,469 + GFF1159 0.38 +0.1 11,470 - GFF1159 0.38 -0.5 11,470 - GFF1159 0.38 -0.7 11,470 - GFF1159 0.38 +0.5 11,496 - GFF1159 0.40 +0.7 11,496 - GFF1159 0.40 -0.4 11,496 - GFF1159 0.40 +0.1 11,504 + GFF1159 0.41 -0.9 11,504 + GFF1159 0.41 +0.3 11,504 + GFF1159 0.41 -0.6 11,517 + GFF1159 0.42 -0.9 11,553 + GFF1159 0.44 -0.2 11,553 + GFF1159 0.44 -0.5 11,555 + GFF1159 0.44 -0.2 11,684 + GFF1159 0.54 +0.2 11,685 - GFF1159 0.54 -0.2 11,685 - GFF1159 0.54 +0.2 11,692 - GFF1159 0.54 -0.2 11,692 - GFF1159 0.54 -0.2 11,692 - GFF1159 0.54 -0.1 11,758 + GFF1159 0.59 -0.5 11,759 - GFF1159 0.59 +0.2 11,759 - GFF1159 0.59 -1.3 11,759 - GFF1159 0.59 +0.1 11,762 + GFF1159 0.59 +0.2 11,762 + GFF1159 0.59 -0.7 11,768 + GFF1159 0.59 -0.4 11,769 - GFF1159 0.59 -1.2 11,771 + GFF1159 0.60 +0.1 11,771 + GFF1159 0.60 +0.8 11,771 + GFF1159 0.60 -0.9 12,220 + -1.1 12,220 + -0.2 12,242 + +0.2 12,243 - -0.1 12,305 + +0.5 12,332 - +0.5 12,342 + -0.1 12,384 + -3.3 12,385 - -1.1 12,385 - -0.1 12,424 + GFF1160 0.15 -1.0 12,659 - GFF1160 0.54 -0.4 12,727 + GFF1160 0.66 -0.3 12,727 + GFF1160 0.66 +0.2 12,744 - GFF1160 0.69 -0.4 12,785 - GFF1160 0.76 -0.5 12,924 + -0.3 12,928 + +0.0 12,934 + -0.2 13,234 - GFF1162 0.16 +0.4 13,257 + GFF1162 0.21 -0.3 13,257 + GFF1162 0.21 -0.1 13,257 + GFF1162 0.21 -0.3
Or see this region's nucleotide sequence