Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF66 and GFF67 overlap by 1 nucleotides GFF67 and GFF68 are separated by 26 nucleotides GFF68 and GFF69 are separated by 107 nucleotides GFF69 and GFF70 overlap by 1 nucleotides
GFF66 - H(+)/Cl(-) exchange transporter ClcA, at 49,007 to 50,428
GFF66
GFF67 - hypothetical protein, at 50,428 to 50,571
GFF67
GFF68 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 50,598 to 51,878
GFF68
GFF69 - Uncharacterized protein YadU in stf fimbrial cluster, at 51,986 to 52,825
GFF69
GFF70 - Minor fimbrial subunit StfG, at 52,825 to 53,355
GFF70
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1 at 49.666 kb on - strand, within GFF66 at 49.736 kb on + strand, within GFF66 at 49.829 kb on - strand, within GFF66 at 49.997 kb on + strand, within GFF66 at 50.166 kb on - strand, within GFF66 at 50.294 kb on - strand at 50.448 kb on - strand, within GFF67 at 50.451 kb on - strand, within GFF67 at 50.553 kb on + strand, within GFF67 at 51.902 kb on - strand at 52.001 kb on - strand at 52.003 kb on + strand at 52.003 kb on + strand at 52.005 kb on + strand at 52.005 kb on + strand at 52.069 kb on + strand at 52.156 kb on + strand, within GFF69 at 52.160 kb on - strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.240 kb on - strand, within GFF69 at 52.244 kb on - strand, within GFF69 at 52.247 kb on + strand, within GFF69 at 52.248 kb on - strand, within GFF69 at 52.248 kb on - strand, within GFF69 at 52.327 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.559 kb on + strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.650 kb on + strand, within GFF69 at 52.651 kb on - strand, within GFF69 at 52.714 kb on + strand, within GFF69 at 52.719 kb on + strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.723 kb on - strand, within GFF69 at 52.828 kb on + strand at 52.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 49,666 - GFF66 0.46 +0.4 49,736 + GFF66 0.51 -0.2 49,829 - GFF66 0.58 -0.4 49,997 + GFF66 0.70 -0.1 50,166 - GFF66 0.82 -0.5 50,294 - +0.6 50,448 - GFF67 0.14 -0.4 50,451 - GFF67 0.16 -0.8 50,553 + GFF67 0.87 -2.0 51,902 - -1.1 52,001 - -0.3 52,003 + -1.5 52,003 + +0.3 52,005 + -1.6 52,005 + +0.0 52,069 + +0.8 52,156 + GFF69 0.20 +0.7 52,160 - GFF69 0.21 +0.9 52,239 + GFF69 0.30 +0.6 52,239 + GFF69 0.30 +0.4 52,239 + GFF69 0.30 -1.2 52,240 - GFF69 0.30 +0.0 52,244 - GFF69 0.31 +0.3 52,247 + GFF69 0.31 -1.5 52,248 - GFF69 0.31 -0.5 52,248 - GFF69 0.31 +0.0 52,327 + GFF69 0.41 +1.4 52,449 + GFF69 0.55 -0.0 52,449 + GFF69 0.55 -0.1 52,449 + GFF69 0.55 -0.5 52,559 + GFF69 0.68 -0.3 52,560 - GFF69 0.68 +0.0 52,560 - GFF69 0.68 +1.1 52,648 - GFF69 0.79 +0.1 52,648 - GFF69 0.79 -0.4 52,650 + GFF69 0.79 -0.2 52,651 - GFF69 0.79 -0.4 52,714 + GFF69 0.87 +0.4 52,719 + GFF69 0.87 -1.5 52,720 - GFF69 0.87 +1.2 52,720 - GFF69 0.87 -0.2 52,723 - GFF69 0.88 +0.4 52,828 + +0.7 52,828 + +1.0
Or see this region's nucleotide sequence