Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF517 and GFF518 are separated by 6 nucleotides GFF518 and GFF519 are separated by 91 nucleotides GFF519 and GFF520 are separated by 375 nucleotides
GFF517 - Oligopeptidase A (EC 3.4.24.70), at 247,096 to 249,138
GFF517
GFF518 - rRNA small subunit methyltransferase J, at 249,145 to 249,903
GFF518
GFF519 - Di/tripeptide permease DtpB, at 249,995 to 251,413
GFF519
GFF520 - Universal stress protein A, at 251,789 to 252,223
GFF520
Position (kb)
249
250
251
252 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 249.192 kb on - strand at 249.649 kb on + strand, within GFF518 at 249.874 kb on - strand at 249.922 kb on + strand at 249.951 kb on - strand at 249.997 kb on - strand at 250.222 kb on + strand, within GFF519 at 250.280 kb on - strand, within GFF519 at 250.312 kb on - strand, within GFF519 at 250.418 kb on - strand, within GFF519 at 250.418 kb on - strand, within GFF519 at 250.432 kb on + strand, within GFF519 at 250.570 kb on + strand, within GFF519 at 250.597 kb on + strand, within GFF519 at 250.726 kb on + strand, within GFF519 at 250.727 kb on - strand, within GFF519 at 250.815 kb on - strand, within GFF519 at 250.917 kb on - strand, within GFF519 at 250.939 kb on - strand, within GFF519 at 251.009 kb on - strand, within GFF519 at 251.019 kb on + strand, within GFF519 at 251.064 kb on + strand, within GFF519 at 251.071 kb on + strand, within GFF519 at 251.099 kb on - strand, within GFF519 at 251.157 kb on - strand, within GFF519 at 251.157 kb on - strand, within GFF519 at 251.306 kb on - strand at 251.394 kb on - strand at 251.394 kb on - strand at 251.458 kb on + strand at 251.459 kb on - strand at 251.459 kb on - strand at 251.459 kb on - strand at 251.480 kb on - strand at 251.533 kb on - strand at 251.618 kb on - strand at 251.745 kb on + strand at 251.745 kb on + strand at 251.747 kb on + strand at 251.748 kb on - strand at 251.788 kb on - strand at 251.788 kb on - strand at 251.788 kb on - strand at 251.920 kb on - strand, within GFF520 at 251.920 kb on - strand, within GFF520 at 251.983 kb on + strand, within GFF520 at 252.052 kb on + strand, within GFF520 at 252.217 kb on - strand at 252.240 kb on + strand at 252.241 kb on - strand at 252.241 kb on - strand at 252.322 kb on - strand at 252.343 kb on + strand at 252.343 kb on + strand at 252.343 kb on + strand at 252.344 kb on - strand at 252.349 kb on + strand at 252.349 kb on + strand at 252.350 kb on - strand at 252.402 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 249,192 - +1.8 249,649 + GFF518 0.66 -0.4 249,874 - -0.0 249,922 + -1.1 249,951 - -0.3 249,997 - +0.4 250,222 + GFF519 0.16 -0.3 250,280 - GFF519 0.20 +0.7 250,312 - GFF519 0.22 -0.6 250,418 - GFF519 0.30 +1.6 250,418 - GFF519 0.30 -1.6 250,432 + GFF519 0.31 -0.0 250,570 + GFF519 0.41 -0.1 250,597 + GFF519 0.42 +0.8 250,726 + GFF519 0.52 -1.8 250,727 - GFF519 0.52 -4.0 250,815 - GFF519 0.58 +0.7 250,917 - GFF519 0.65 +0.4 250,939 - GFF519 0.67 -0.6 251,009 - GFF519 0.71 -1.0 251,019 + GFF519 0.72 +0.1 251,064 + GFF519 0.75 -0.1 251,071 + GFF519 0.76 +0.0 251,099 - GFF519 0.78 -0.4 251,157 - GFF519 0.82 -0.6 251,157 - GFF519 0.82 +0.1 251,306 - -0.3 251,394 - -0.5 251,394 - +0.1 251,458 + +0.6 251,459 - +1.1 251,459 - +0.3 251,459 - +0.1 251,480 - -0.4 251,533 - +0.2 251,618 - +0.7 251,745 + -0.3 251,745 + +0.3 251,747 + -1.8 251,748 - +0.1 251,788 - +0.1 251,788 - -0.1 251,788 - +0.5 251,920 - GFF520 0.30 +0.6 251,920 - GFF520 0.30 -1.1 251,983 + GFF520 0.45 +0.6 252,052 + GFF520 0.60 +0.5 252,217 - -0.2 252,240 + -1.6 252,241 - +0.1 252,241 - +0.2 252,322 - +0.7 252,343 + +0.1 252,343 + -1.0 252,343 + -0.2 252,344 - -0.6 252,349 + -0.0 252,349 + +0.6 252,350 - +0.2 252,402 - -0.2
Or see this region's nucleotide sequence