Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4762 and GFF4763 are separated by 24 nucleotides GFF4763 and GFF4764 overlap by 4 nucleotides
GFF4762 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 68,183 to 70,240
GFF4762
GFF4763 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 70,265 to 71,383
GFF4763
GFF4764 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 71,380 to 72,924
GFF4764
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 69.391 kb on + strand, within GFF4762 at 69.449 kb on - strand, within GFF4762 at 69.491 kb on - strand, within GFF4762 at 69.550 kb on + strand, within GFF4762 at 69.551 kb on - strand, within GFF4762 at 69.551 kb on - strand, within GFF4762 at 69.553 kb on - strand, within GFF4762 at 69.752 kb on - strand, within GFF4762 at 69.752 kb on - strand, within GFF4762 at 69.800 kb on - strand, within GFF4762 at 69.883 kb on + strand, within GFF4762 at 70.071 kb on + strand at 70.209 kb on - strand at 70.334 kb on - strand at 70.334 kb on - strand at 70.530 kb on - strand, within GFF4763 at 70.601 kb on + strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.753 kb on - strand, within GFF4763 at 70.829 kb on + strand, within GFF4763 at 71.009 kb on + strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.010 kb on - strand, within GFF4763 at 71.076 kb on - strand, within GFF4763 at 71.084 kb on + strand, within GFF4763 at 71.302 kb on + strand at 71.302 kb on + strand at 71.302 kb on + strand at 71.302 kb on + strand at 71.431 kb on + strand at 71.566 kb on - strand, within GFF4764 at 71.592 kb on + strand, within GFF4764 at 71.704 kb on - strand, within GFF4764 at 71.738 kb on - strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.748 kb on + strand, within GFF4764 at 71.803 kb on + strand, within GFF4764 at 71.804 kb on - strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.852 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.857 kb on + strand, within GFF4764 at 71.858 kb on - strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.877 kb on + strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 71.878 kb on - strand, within GFF4764 at 72.037 kb on + strand, within GFF4764 at 72.078 kb on + strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.270 kb on + strand, within GFF4764 at 72.271 kb on - strand, within GFF4764
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 69,391 + GFF4762 0.59 +0.8 69,449 - GFF4762 0.62 -0.2 69,491 - GFF4762 0.64 +0.7 69,550 + GFF4762 0.66 +0.8 69,551 - GFF4762 0.66 +0.0 69,551 - GFF4762 0.66 -0.1 69,553 - GFF4762 0.67 -1.9 69,752 - GFF4762 0.76 +0.1 69,752 - GFF4762 0.76 +0.1 69,800 - GFF4762 0.79 +1.0 69,883 + GFF4762 0.83 -0.5 70,071 + -2.4 70,209 - -0.7 70,334 - +0.2 70,334 - +0.1 70,530 - GFF4763 0.24 -1.1 70,601 + GFF4763 0.30 +0.3 70,753 - GFF4763 0.44 -0.3 70,753 - GFF4763 0.44 +2.0 70,753 - GFF4763 0.44 +1.1 70,829 + GFF4763 0.50 +0.1 71,009 + GFF4763 0.66 +0.3 71,010 - GFF4763 0.67 -0.1 71,010 - GFF4763 0.67 +0.1 71,076 - GFF4763 0.72 -0.2 71,084 + GFF4763 0.73 +0.6 71,302 + -3.3 71,302 + -0.6 71,302 + +0.6 71,302 + -0.6 71,431 + +0.1 71,566 - GFF4764 0.12 +0.4 71,592 + GFF4764 0.14 +0.1 71,704 - GFF4764 0.21 -0.1 71,738 - GFF4764 0.23 -2.0 71,748 + GFF4764 0.24 +0.9 71,748 + GFF4764 0.24 +1.4 71,748 + GFF4764 0.24 -0.5 71,803 + GFF4764 0.27 +0.2 71,804 - GFF4764 0.27 -0.0 71,852 + GFF4764 0.31 +0.2 71,852 + GFF4764 0.31 +1.1 71,852 + GFF4764 0.31 +1.9 71,857 + GFF4764 0.31 +0.7 71,857 + GFF4764 0.31 +0.6 71,857 + GFF4764 0.31 +0.2 71,858 - GFF4764 0.31 +1.6 71,877 + GFF4764 0.32 +0.9 71,877 + GFF4764 0.32 -2.8 71,877 + GFF4764 0.32 +1.0 71,877 + GFF4764 0.32 -0.3 71,878 - GFF4764 0.32 +0.3 71,878 - GFF4764 0.32 -0.4 71,878 - GFF4764 0.32 -0.4 72,037 + GFF4764 0.43 -0.3 72,078 + GFF4764 0.45 +0.8 72,083 - GFF4764 0.46 +0.5 72,083 - GFF4764 0.46 -0.4 72,270 + GFF4764 0.58 +0.1 72,271 - GFF4764 0.58 +0.4
Or see this region's nucleotide sequence