Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4554 and GFF4555 are separated by 8 nucleotides GFF4555 and GFF4556 are separated by 14 nucleotides GFF4556 and GFF4557 are separated by 287 nucleotides GFF4557 and GFF4558 are separated by 22 nucleotides
GFF4554 - DNA polymerase III delta subunit (EC 2.7.7.7), at 337,703 to 338,725
GFF4554
GFF4555 - LPS-assembly lipoprotein RlpB precursor (Rare lipoprotein B), at 338,734 to 339,324
GFF4555
GFF4556 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 339,339 to 341,921
GFF4556
GFF4557 - Hydrolase, UxaA family, at 342,209 to 342,499
GFF4557
GFF4558 - Altronate hydrolase (EC 4.2.1.7), at 342,522 to 343,688
GFF4558
Position (kb) 339
340
341
342 Strain fitness (log2 ratio) -2
-1
0
1 at 342.262 kb on + strand, within GFF4557 at 342.263 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.395 kb on - strand, within GFF4557 at 342.398 kb on + strand, within GFF4557 at 342.399 kb on - strand, within GFF4557 at 342.447 kb on - strand, within GFF4557 at 342.447 kb on - strand, within GFF4557 at 342.582 kb on + strand at 342.729 kb on - strand, within GFF4558 at 342.818 kb on + strand, within GFF4558 at 342.875 kb on + strand, within GFF4558 at 342.876 kb on - strand, within GFF4558
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 342,262 + GFF4557 0.18 -1.8 342,263 - GFF4557 0.19 +1.3 342,395 - GFF4557 0.64 +0.0 342,395 - GFF4557 0.64 -0.4 342,395 - GFF4557 0.64 +1.0 342,398 + GFF4557 0.65 +0.5 342,399 - GFF4557 0.65 +0.4 342,447 - GFF4557 0.82 +0.1 342,447 - GFF4557 0.82 -0.1 342,582 + -0.2 342,729 - GFF4558 0.18 -0.7 342,818 + GFF4558 0.25 -0.2 342,875 + GFF4558 0.30 +0.6 342,876 - GFF4558 0.30 +0.6
Or see this region's nucleotide sequence