Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3873 and GFF3874 are separated by 15 nucleotides GFF3874 and GFF3875 are separated by 23 nucleotides GFF3875 and GFF3876 are separated by 2 nucleotides GFF3876 and GFF3877 are separated by 2 nucleotides
GFF3873 - Colanic acid biosynthesis glycosyl transferase WcaE, at 149,788 to 150,534
GFF3873
GFF3874 - Colanic acid biosynthesis acetyltransferase WcaF (EC 2.3.1.-), at 150,550 to 151,104
GFF3874
GFF3875 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 151,128 to 152,249
GFF3875
GFF3876 - GDP-L-fucose synthetase (EC 1.1.1.271), at 152,252 to 153,217
GFF3876
GFF3877 - GDP-mannose mannosyl hydrolase (EC 3.6.1.-), at 153,220 to 153,693
GFF3877
Position (kb) 151
152
153 Strain fitness (log2 ratio) -1
0
1 at 150.200 kb on - strand, within GFF3873 at 150.200 kb on - strand, within GFF3873 at 150.662 kb on + strand, within GFF3874 at 150.810 kb on + strand, within GFF3874 at 151.023 kb on + strand, within GFF3874 at 151.265 kb on + strand, within GFF3875 at 151.377 kb on + strand, within GFF3875 at 151.377 kb on + strand, within GFF3875 at 151.378 kb on - strand, within GFF3875 at 151.378 kb on - strand, within GFF3875 at 151.656 kb on - strand, within GFF3875 at 151.824 kb on + strand, within GFF3875 at 151.849 kb on + strand, within GFF3875 at 152.257 kb on + strand at 152.578 kb on + strand, within GFF3876 at 152.579 kb on - strand, within GFF3876 at 152.658 kb on - strand, within GFF3876 at 152.699 kb on + strand, within GFF3876 at 152.700 kb on - strand, within GFF3876 at 152.914 kb on + strand, within GFF3876 at 153.176 kb on + strand at 153.177 kb on - strand at 153.177 kb on - strand at 153.227 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 150,200 - GFF3873 0.55 +0.1 150,200 - GFF3873 0.55 +0.5 150,662 + GFF3874 0.20 -0.5 150,810 + GFF3874 0.47 -0.5 151,023 + GFF3874 0.85 +1.4 151,265 + GFF3875 0.12 -1.7 151,377 + GFF3875 0.22 +0.2 151,377 + GFF3875 0.22 -1.4 151,378 - GFF3875 0.22 +0.1 151,378 - GFF3875 0.22 -0.3 151,656 - GFF3875 0.47 -0.4 151,824 + GFF3875 0.62 -0.2 151,849 + GFF3875 0.64 -0.4 152,257 + +0.7 152,578 + GFF3876 0.34 -1.5 152,579 - GFF3876 0.34 +0.6 152,658 - GFF3876 0.42 +0.4 152,699 + GFF3876 0.46 +0.1 152,700 - GFF3876 0.46 -0.5 152,914 + GFF3876 0.69 -0.2 153,176 + -0.1 153,177 - -0.3 153,177 - +0.5 153,227 + -1.1
Or see this region's nucleotide sequence