Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3487 and GFF3488 are separated by 238 nucleotides GFF3488 and GFF3489 overlap by 41 nucleotides GFF3489 and GFF3490 are separated by 72 nucleotides
GFF3487 - NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 240,689 to 242,032
GFF3487
GFF3488 - FIG00638396: hypothetical protein, at 242,271 to 242,546
GFF3488
GFF3489 - 5-methyl-dCTP pyrophosphohydrolase (EC 3.6.1.-), at 242,506 to 242,922
GFF3489
GFF3490 - Exodeoxyribonuclease III (EC 3.1.11.2), at 242,995 to 243,801
GFF3490
Position (kb) 242
243 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 241.761 kb on + strand, within GFF3487 at 241.992 kb on - strand at 242.033 kb on - strand at 242.033 kb on - strand at 242.090 kb on + strand at 242.110 kb on - strand at 242.234 kb on + strand at 242.245 kb on + strand at 242.246 kb on - strand at 242.641 kb on + strand, within GFF3489 at 242.723 kb on + strand, within GFF3489 at 242.761 kb on + strand, within GFF3489 at 242.827 kb on + strand, within GFF3489 at 243.059 kb on + strand at 243.361 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.495 kb on + strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.496 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.529 kb on - strand, within GFF3490 at 243.636 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.648 kb on - strand, within GFF3490 at 243.801 kb on + strand at 243.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 241,761 + GFF3487 0.80 +0.2 241,992 - -2.1 242,033 - +0.2 242,033 - -0.8 242,090 + +0.9 242,110 - -0.1 242,234 + -0.4 242,245 + -0.1 242,246 - +0.5 242,641 + GFF3489 0.32 +0.7 242,723 + GFF3489 0.52 +1.0 242,761 + GFF3489 0.61 -0.2 242,827 + GFF3489 0.77 -0.1 243,059 + -1.5 243,361 + GFF3490 0.45 -0.4 243,495 + GFF3490 0.62 -1.6 243,495 + GFF3490 0.62 -0.5 243,495 + GFF3490 0.62 -0.3 243,496 - GFF3490 0.62 -0.7 243,496 - GFF3490 0.62 -0.6 243,529 - GFF3490 0.66 -1.4 243,529 - GFF3490 0.66 -0.7 243,636 - GFF3490 0.79 -1.0 243,648 - GFF3490 0.81 -0.9 243,648 - GFF3490 0.81 -0.7 243,801 + -2.8 243,898 + +0.7
Or see this region's nucleotide sequence