Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF272 and GFF273 are separated by 9 nucleotides GFF273 and GFF274 are separated by 189 nucleotides
GFF272 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 46,971 to 48,134
GFF272
GFF273 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 48,144 to 50,333
GFF273
GFF274 - Xaa-Pro dipeptidase PepQ (EC 3.4.13.9), at 50,523 to 51,854
GFF274
Position (kb)
48
49
50
51 Strain fitness (log2 ratio)
-1
0
1 at 47.385 kb on + strand, within GFF272 at 47.519 kb on - strand, within GFF272 at 47.519 kb on - strand, within GFF272 at 47.925 kb on - strand, within GFF272 at 47.978 kb on + strand, within GFF272 at 47.979 kb on - strand, within GFF272 at 47.999 kb on + strand, within GFF272 at 48.011 kb on + strand, within GFF272 at 48.026 kb on + strand at 48.107 kb on + strand at 48.145 kb on + strand at 48.197 kb on - strand at 48.244 kb on + strand at 48.266 kb on - strand at 48.368 kb on + strand, within GFF273 at 48.443 kb on + strand, within GFF273 at 48.453 kb on + strand, within GFF273 at 49.431 kb on - strand, within GFF273 at 49.437 kb on + strand, within GFF273 at 49.437 kb on + strand, within GFF273 at 49.488 kb on - strand, within GFF273 at 49.511 kb on + strand, within GFF273 at 49.625 kb on + strand, within GFF273 at 49.913 kb on + strand, within GFF273 at 49.914 kb on - strand, within GFF273 at 50.032 kb on - strand, within GFF273 at 50.057 kb on + strand, within GFF273 at 50.058 kb on - strand, within GFF273 at 50.156 kb on + strand at 50.309 kb on - strand at 50.325 kb on - strand at 50.326 kb on + strand at 50.326 kb on + strand at 50.618 kb on + strand at 50.793 kb on - strand, within GFF274 at 50.982 kb on + strand, within GFF274 at 50.983 kb on - strand, within GFF274 at 50.991 kb on + strand, within GFF274 at 50.991 kb on + strand, within GFF274 at 50.992 kb on - strand, within GFF274 at 51.162 kb on + strand, within GFF274 at 51.207 kb on + strand, within GFF274
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 47,385 + GFF272 0.36 +0.8 47,519 - GFF272 0.47 -0.6 47,519 - GFF272 0.47 -0.9 47,925 - GFF272 0.82 +0.0 47,978 + GFF272 0.87 -0.0 47,979 - GFF272 0.87 -1.3 47,999 + GFF272 0.88 -0.2 48,011 + GFF272 0.89 +0.0 48,026 + +0.7 48,107 + +0.4 48,145 + +0.4 48,197 - +0.6 48,244 + -0.1 48,266 - +0.2 48,368 + GFF273 0.10 -0.5 48,443 + GFF273 0.14 -0.6 48,453 + GFF273 0.14 +0.2 49,431 - GFF273 0.59 -0.1 49,437 + GFF273 0.59 +0.3 49,437 + GFF273 0.59 -0.1 49,488 - GFF273 0.61 +0.1 49,511 + GFF273 0.62 +0.8 49,625 + GFF273 0.68 -0.3 49,913 + GFF273 0.81 +0.7 49,914 - GFF273 0.81 -0.3 50,032 - GFF273 0.86 -1.5 50,057 + GFF273 0.87 -0.0 50,058 - GFF273 0.87 -0.5 50,156 + +0.3 50,309 - -0.0 50,325 - +0.2 50,326 + -0.2 50,326 + +0.1 50,618 + +0.0 50,793 - GFF274 0.20 +0.2 50,982 + GFF274 0.34 +0.3 50,983 - GFF274 0.35 -0.0 50,991 + GFF274 0.35 -1.4 50,991 + GFF274 0.35 -0.9 50,992 - GFF274 0.35 +0.5 51,162 + GFF274 0.48 +0.0 51,207 + GFF274 0.51 -0.2
Or see this region's nucleotide sequence