Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2161 and GFF2162 are separated by 222 nucleotides GFF2162 and GFF2163 are separated by 333 nucleotides GFF2163 and GFF2164 are separated by 364 nucleotides
GFF2161 - GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2), at 7,002 to 8,579
GFF2161
GFF2162 - Mobile element protein, at 8,802 to 9,092
GFF2162
GFF2163 - FIG01047222: hypothetical protein, at 9,426 to 9,617
GFF2163
GFF2164 - Putative inner membrane protein, at 9,982 to 10,125
GFF2164
Position (kb)
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 8.441 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.740 kb on - strand at 8.740 kb on - strand at 8.813 kb on + strand at 9.267 kb on - strand at 9.288 kb on + strand at 9.444 kb on - strand at 9.485 kb on + strand, within GFF2163 at 9.486 kb on - strand, within GFF2163 at 9.586 kb on - strand, within GFF2163 at 9.648 kb on - strand at 9.650 kb on - strand at 9.671 kb on - strand at 9.671 kb on - strand at 9.674 kb on + strand at 9.674 kb on + strand at 9.675 kb on - strand at 9.684 kb on + strand at 9.722 kb on + strand at 9.726 kb on + strand at 9.882 kb on + strand at 9.984 kb on - strand at 10.238 kb on - strand at 10.319 kb on - strand at 10.344 kb on - strand at 10.503 kb on + strand at 10.586 kb on - strand at 10.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 8,441 + -1.8 8,739 + -0.1 8,739 + +0.0 8,740 - -0.9 8,740 - +0.1 8,813 + -0.8 9,267 - -0.2 9,288 + -1.5 9,444 - -2.9 9,485 + GFF2163 0.31 +0.7 9,486 - GFF2163 0.31 +0.7 9,586 - GFF2163 0.83 +1.1 9,648 - -0.4 9,650 - +0.8 9,671 - +0.8 9,671 - -0.1 9,674 + -0.1 9,674 + +0.6 9,675 - -1.3 9,684 + +0.1 9,722 + -1.7 9,726 + +0.4 9,882 + +1.0 9,984 - +1.7 10,238 - -0.3 10,319 - +1.3 10,344 - +1.1 10,503 + +0.4 10,586 - +1.7 10,608 + -0.2
Or see this region's nucleotide sequence