Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4500 and GFF4501 are separated by 9 nucleotides GFF4501 and GFF4502 are separated by 13 nucleotides GFF4502 and GFF4503 overlap by 1 nucleotides
GFF4500 - Isochorismate synthase (EC 5.4.4.2) of siderophore biosynthesis, at 286,469 to 287,644
GFF4500
GFF4501 - 2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore, at 287,654 to 289,264
GFF4501
GFF4502 - Isochorismatase (EC 3.3.2.1) of siderophore biosynthesis, at 289,278 to 290,135
GFF4502
GFF4503 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (EC 1.3.1.28) of siderophore biosynthesis, at 290,135 to 290,890
GFF4503
Position (kb) 287
288
289
290 Strain fitness (log2 ratio) -2
-1
0
1 at 286.685 kb on - strand, within GFF4500 at 286.929 kb on + strand, within GFF4500 at 286.929 kb on + strand, within GFF4500 at 286.933 kb on + strand, within GFF4500 at 287.254 kb on - strand, within GFF4500 at 287.642 kb on + strand at 287.661 kb on + strand at 287.661 kb on + strand at 287.715 kb on - strand at 287.847 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.912 kb on + strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.913 kb on - strand, within GFF4501 at 287.946 kb on + strand, within GFF4501 at 287.947 kb on - strand, within GFF4501 at 287.990 kb on - strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.043 kb on + strand, within GFF4501 at 288.044 kb on - strand, within GFF4501 at 288.044 kb on - strand, within GFF4501 at 288.229 kb on + strand, within GFF4501 at 288.278 kb on + strand, within GFF4501 at 288.356 kb on - strand, within GFF4501 at 288.359 kb on + strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.360 kb on - strand, within GFF4501 at 288.514 kb on - strand, within GFF4501 at 288.994 kb on - strand, within GFF4501 at 289.083 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.093 kb on - strand, within GFF4501 at 289.120 kb on - strand at 289.302 kb on + strand at 289.388 kb on + strand, within GFF4502 at 289.389 kb on - strand, within GFF4502 at 289.478 kb on + strand, within GFF4502 at 289.479 kb on - strand, within GFF4502 at 289.503 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.556 kb on + strand, within GFF4502 at 289.557 kb on - strand, within GFF4502 at 289.604 kb on - strand, within GFF4502 at 289.654 kb on - strand, within GFF4502 at 289.739 kb on + strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 289.852 kb on - strand, within GFF4502 at 290.015 kb on - strand, within GFF4502 at 290.051 kb on - strand at 290.133 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 286,685 - GFF4500 0.18 -2.2 286,929 + GFF4500 0.39 -0.5 286,929 + GFF4500 0.39 +0.9 286,933 + GFF4500 0.39 +0.2 287,254 - GFF4500 0.67 -2.7 287,642 + +0.4 287,661 + -1.1 287,661 + -0.3 287,715 - -0.3 287,847 + GFF4501 0.12 -1.8 287,912 + GFF4501 0.16 -0.6 287,912 + GFF4501 0.16 -0.8 287,912 + GFF4501 0.16 -0.1 287,913 - GFF4501 0.16 -0.2 287,913 - GFF4501 0.16 -0.6 287,913 - GFF4501 0.16 -0.5 287,913 - GFF4501 0.16 +0.2 287,946 + GFF4501 0.18 -0.3 287,947 - GFF4501 0.18 +0.0 287,990 - GFF4501 0.21 -0.8 288,043 + GFF4501 0.24 -0.0 288,043 + GFF4501 0.24 -0.5 288,043 + GFF4501 0.24 -0.4 288,044 - GFF4501 0.24 -1.7 288,044 - GFF4501 0.24 -0.1 288,229 + GFF4501 0.36 +0.6 288,278 + GFF4501 0.39 -0.1 288,356 - GFF4501 0.44 -1.1 288,359 + GFF4501 0.44 +0.2 288,360 - GFF4501 0.44 -0.3 288,360 - GFF4501 0.44 +0.1 288,514 - GFF4501 0.53 +0.4 288,994 - GFF4501 0.83 -0.8 289,083 - GFF4501 0.89 -0.4 289,093 - GFF4501 0.89 -1.1 289,093 - GFF4501 0.89 +0.3 289,120 - -0.9 289,302 + -0.1 289,388 + GFF4502 0.13 -0.7 289,389 - GFF4502 0.13 -0.1 289,478 + GFF4502 0.23 +0.7 289,479 - GFF4502 0.23 -0.1 289,503 + GFF4502 0.26 +0.1 289,556 + GFF4502 0.32 +0.0 289,556 + GFF4502 0.32 +0.3 289,556 + GFF4502 0.32 +0.5 289,557 - GFF4502 0.33 -0.7 289,604 - GFF4502 0.38 +0.3 289,654 - GFF4502 0.44 -0.7 289,739 + GFF4502 0.54 -0.2 289,852 - GFF4502 0.67 -1.1 289,852 - GFF4502 0.67 -1.9 290,015 - GFF4502 0.86 -0.6 290,051 - -0.3 290,133 + -0.5
Or see this region's nucleotide sequence