Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF182 and GFF183 are separated by 173 nucleotides GFF183 and GFF184 are separated by 11 nucleotides GFF184 and GFF185 are separated by 94 nucleotides
GFF182 - DNA polymerase II (EC 2.7.7.7), at 173,657 to 176,008
GFF182
GFF183 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 176,182 to 179,088
GFF183
GFF184 - Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70), at 179,100 to 179,759
GFF184
GFF185 - DnaJ-like protein DjlA, at 179,854 to 180,666
GFF185
Position (kb) 176
177
178
179
180 Strain fitness (log2 ratio) -2
-1
0
1 at 175.284 kb on - strand, within GFF182 at 175.284 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.614 kb on - strand, within GFF182 at 175.649 kb on + strand, within GFF182 at 175.650 kb on - strand, within GFF182 at 175.731 kb on + strand, within GFF182 at 175.732 kb on - strand, within GFF182 at 175.845 kb on - strand at 175.845 kb on - strand at 175.902 kb on - strand at 175.911 kb on - strand at 175.927 kb on - strand at 175.976 kb on - strand at 175.980 kb on + strand at 176.085 kb on + strand at 176.085 kb on + strand at 176.086 kb on - strand at 176.160 kb on - strand at 176.167 kb on + strand at 176.190 kb on + strand at 176.277 kb on - strand at 176.364 kb on + strand at 176.402 kb on + strand at 176.429 kb on - strand at 176.658 kb on + strand, within GFF183 at 176.676 kb on - strand, within GFF183 at 176.727 kb on + strand, within GFF183 at 177.067 kb on + strand, within GFF183 at 177.067 kb on + strand, within GFF183 at 177.068 kb on - strand, within GFF183 at 177.069 kb on + strand, within GFF183 at 177.070 kb on - strand, within GFF183 at 177.352 kb on - strand, within GFF183 at 177.488 kb on + strand, within GFF183 at 177.553 kb on - strand, within GFF183 at 177.580 kb on - strand, within GFF183 at 177.891 kb on + strand, within GFF183 at 177.891 kb on + strand, within GFF183 at 177.961 kb on - strand, within GFF183 at 178.109 kb on - strand, within GFF183 at 178.138 kb on + strand, within GFF183 at 178.461 kb on + strand, within GFF183 at 178.513 kb on - strand, within GFF183 at 178.707 kb on + strand, within GFF183 at 178.707 kb on + strand, within GFF183 at 178.707 kb on + strand, within GFF183 at 178.708 kb on - strand, within GFF183 at 178.708 kb on - strand, within GFF183 at 178.708 kb on - strand, within GFF183 at 178.708 kb on - strand, within GFF183 at 178.708 kb on - strand, within GFF183 at 179.068 kb on - strand at 179.168 kb on + strand, within GFF184 at 179.237 kb on + strand, within GFF184 at 179.386 kb on - strand, within GFF184 at 179.386 kb on - strand, within GFF184 at 179.437 kb on + strand, within GFF184 at 179.516 kb on + strand, within GFF184 at 179.600 kb on + strand, within GFF184 at 179.600 kb on + strand, within GFF184 at 179.600 kb on + strand, within GFF184 at 179.641 kb on - strand, within GFF184 at 179.881 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 175,284 - GFF182 0.69 -0.4 175,284 - GFF182 0.69 -0.1 175,614 - GFF182 0.83 +0.4 175,614 - GFF182 0.83 +0.3 175,614 - GFF182 0.83 -0.4 175,614 - GFF182 0.83 +0.2 175,649 + GFF182 0.85 +0.7 175,650 - GFF182 0.85 -0.5 175,731 + GFF182 0.88 +0.4 175,732 - GFF182 0.88 -0.2 175,845 - -1.0 175,845 - +0.4 175,902 - +0.7 175,911 - -0.3 175,927 - -0.2 175,976 - +0.1 175,980 + +0.1 176,085 + -2.1 176,085 + +0.1 176,086 - -0.4 176,160 - -0.5 176,167 + -0.5 176,190 + +0.0 176,277 - -1.2 176,364 + -2.0 176,402 + -1.1 176,429 - -0.7 176,658 + GFF183 0.16 -0.6 176,676 - GFF183 0.17 +0.5 176,727 + GFF183 0.19 -0.7 177,067 + GFF183 0.30 -1.8 177,067 + GFF183 0.30 -0.3 177,068 - GFF183 0.30 -0.4 177,069 + GFF183 0.31 -0.9 177,070 - GFF183 0.31 -0.5 177,352 - GFF183 0.40 -0.3 177,488 + GFF183 0.45 -0.4 177,553 - GFF183 0.47 -0.5 177,580 - GFF183 0.48 -0.2 177,891 + GFF183 0.59 -0.2 177,891 + GFF183 0.59 -0.1 177,961 - GFF183 0.61 -0.8 178,109 - GFF183 0.66 -0.5 178,138 + GFF183 0.67 -2.2 178,461 + GFF183 0.78 -0.2 178,513 - GFF183 0.80 +0.3 178,707 + GFF183 0.87 -1.1 178,707 + GFF183 0.87 -1.1 178,707 + GFF183 0.87 -0.3 178,708 - GFF183 0.87 -1.9 178,708 - GFF183 0.87 -1.0 178,708 - GFF183 0.87 -0.8 178,708 - GFF183 0.87 -0.3 178,708 - GFF183 0.87 +0.1 179,068 - -1.8 179,168 + GFF184 0.10 -0.2 179,237 + GFF184 0.21 -0.2 179,386 - GFF184 0.43 -0.4 179,386 - GFF184 0.43 +0.0 179,437 + GFF184 0.51 -0.1 179,516 + GFF184 0.63 +0.2 179,600 + GFF184 0.76 -0.4 179,600 + GFF184 0.76 -0.2 179,600 + GFF184 0.76 -0.2 179,641 - GFF184 0.82 -1.5 179,881 + -0.1
Or see this region's nucleotide sequence