Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsN and yhbJ are separated by 45 nucleotides yhbJ and npr overlap by 4 nucleotides npr and yrbL are separated by 213 nucleotides yrbL and mtgA overlap by 4 nucleotides
b3204: ptsN - sugar-specific enzyme IIA component of PTS (NCBI), at 3,344,600 to 3,345,091
ptsN
b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991
yhbJ
b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260
npr
b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106
yrbL
b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831
mtgA
Position (kb)
3345
3346
3347 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.026 kb on + strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.034 kb on - strand, within ptsN at 3345.066 kb on + strand at 3345.072 kb on + strand at 3345.072 kb on + strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.102 kb on - strand at 3345.116 kb on - strand at 3345.116 kb on - strand at 3345.139 kb on - strand at 3345.140 kb on + strand at 3345.231 kb on + strand, within yhbJ at 3345.249 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.264 kb on - strand, within yhbJ at 3345.305 kb on - strand, within yhbJ at 3345.343 kb on + strand, within yhbJ at 3345.396 kb on - strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.698 kb on + strand, within yhbJ at 3345.735 kb on + strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.863 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.892 kb on - strand, within yhbJ at 3345.909 kb on - strand at 3345.953 kb on + strand at 3346.034 kb on - strand, within npr at 3346.147 kb on + strand, within npr at 3346.173 kb on - strand, within npr at 3346.174 kb on + strand, within npr at 3346.174 kb on + strand, within npr at 3346.186 kb on - strand, within npr at 3346.196 kb on - strand, within npr at 3346.286 kb on + strand at 3346.286 kb on + strand at 3346.444 kb on - strand at 3346.487 kb on - strand at 3346.655 kb on + strand, within yrbL at 3346.688 kb on - strand, within yrbL at 3346.697 kb on - strand, within yrbL at 3346.795 kb on + strand, within yrbL at 3346.809 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.921 kb on + strand, within yrbL at 3346.932 kb on - strand, within yrbL at 3346.965 kb on - strand, within yrbL at 3346.972 kb on + strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.980 kb on - strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3346.986 kb on + strand, within yrbL at 3347.046 kb on + strand at 3347.086 kb on - strand at 3347.092 kb on - strand at 3347.111 kb on + strand at 3347.151 kb on - strand at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.178 kb on + strand, within mtgA at 3347.184 kb on + strand, within mtgA at 3347.192 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.205 kb on - strand, within mtgA at 3347.226 kb on + strand, within mtgA at 3347.226 kb on + strand, within mtgA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 3,345,026 + ptsN b3204 0.87 -0.3 3,345,026 + ptsN b3204 0.87 +1.3 3,345,026 + ptsN b3204 0.87 +0.5 3,345,034 - ptsN b3204 0.88 -0.9 3,345,034 - ptsN b3204 0.88 -0.1 3,345,034 - ptsN b3204 0.88 -0.8 3,345,034 - ptsN b3204 0.88 -0.5 3,345,066 + +1.6 3,345,072 + +0.4 3,345,072 + +0.5 3,345,102 - -1.9 3,345,102 - -1.2 3,345,102 - -0.1 3,345,116 - -0.4 3,345,116 - -0.8 3,345,139 - -1.8 3,345,140 + -1.2 3,345,231 + yhbJ b3205 0.11 -0.7 3,345,249 - yhbJ b3205 0.13 -1.1 3,345,264 - yhbJ b3205 0.15 -1.6 3,345,264 - yhbJ b3205 0.15 +0.6 3,345,305 - yhbJ b3205 0.20 -1.1 3,345,343 + yhbJ b3205 0.24 -3.6 3,345,396 - yhbJ b3205 0.30 -0.8 3,345,698 + yhbJ b3205 0.66 -0.2 3,345,698 + yhbJ b3205 0.66 -0.5 3,345,735 + yhbJ b3205 0.70 -0.7 3,345,863 - yhbJ b3205 0.85 -0.5 3,345,863 - yhbJ b3205 0.85 -1.0 3,345,892 - yhbJ b3205 0.88 -1.4 3,345,892 - yhbJ b3205 0.88 -0.5 3,345,909 - -0.8 3,345,953 + -1.4 3,346,034 - npr b3206 0.17 -1.9 3,346,147 + npr b3206 0.58 +0.1 3,346,173 - npr b3206 0.68 -0.8 3,346,174 + npr b3206 0.68 -0.4 3,346,174 + npr b3206 0.68 -2.1 3,346,186 - npr b3206 0.73 -0.5 3,346,196 - npr b3206 0.76 -0.8 3,346,286 + +0.0 3,346,286 + -0.8 3,346,444 - +0.0 3,346,487 - +0.3 3,346,655 + yrbL b3207 0.29 +0.2 3,346,688 - yrbL b3207 0.34 -1.2 3,346,697 - yrbL b3207 0.35 -0.1 3,346,795 + yrbL b3207 0.51 -0.2 3,346,809 + yrbL b3207 0.53 +0.3 3,346,921 + yrbL b3207 0.71 -0.3 3,346,921 + yrbL b3207 0.71 +0.8 3,346,932 - yrbL b3207 0.72 +0.1 3,346,965 - yrbL b3207 0.78 +0.4 3,346,972 + yrbL b3207 0.79 -0.2 3,346,980 - yrbL b3207 0.80 +0.4 3,346,980 - yrbL b3207 0.80 -0.6 3,346,986 + yrbL b3207 0.81 +0.1 3,346,986 + yrbL b3207 0.81 +0.3 3,347,046 + -0.0 3,347,086 - +0.1 3,347,092 - +0.2 3,347,111 + -0.2 3,347,151 - +0.1 3,347,178 + mtgA b3208 0.10 +0.3 3,347,178 + mtgA b3208 0.10 +0.4 3,347,178 + mtgA b3208 0.10 +0.2 3,347,178 + mtgA b3208 0.10 -0.5 3,347,184 + mtgA b3208 0.11 -0.1 3,347,192 - mtgA b3208 0.12 -0.7 3,347,205 - mtgA b3208 0.14 +0.6 3,347,205 - mtgA b3208 0.14 +0.4 3,347,226 + mtgA b3208 0.17 -0.2 3,347,226 + mtgA b3208 0.17 +0.4
Or see this region's nucleotide sequence