Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbE and cysC are separated by 49 nucleotides cysC and cysN overlap by 1 nucleotides cysN and cysD are separated by 1 nucleotides
b2749: ygbE - conserved inner membrane protein (NCBI), at 2,871,036 to 2,871,359
ygbE
b2750: cysC - adenylylsulfate kinase (NCBI), at 2,871,409 to 2,872,014
cysC
b2751: cysN - sulfate adenylyltransferase subunit 1 (NCBI), at 2,872,014 to 2,873,441
cysN
b2752: cysD - sulfate adenylyltransferase subunit 2 (NCBI), at 2,873,443 to 2,874,351
cysD
Position (kb)
2872
2873
2874 Strain fitness (log2 ratio)
-2
-1
0
1 at 2871.097 kb on + strand, within ygbE at 2871.140 kb on - strand, within ygbE at 2871.145 kb on - strand, within ygbE at 2871.145 kb on - strand, within ygbE at 2871.149 kb on + strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.197 kb on - strand, within ygbE at 2871.207 kb on - strand, within ygbE at 2871.207 kb on - strand, within ygbE at 2871.248 kb on + strand, within ygbE at 2871.285 kb on - strand, within ygbE at 2871.290 kb on + strand, within ygbE at 2871.311 kb on + strand, within ygbE at 2871.391 kb on + strand at 2871.421 kb on - strand at 2871.534 kb on - strand, within cysC at 2871.692 kb on - strand, within cysC at 2871.703 kb on + strand, within cysC at 2871.807 kb on - strand, within cysC at 2871.807 kb on - strand, within cysC at 2871.866 kb on + strand, within cysC at 2871.866 kb on + strand, within cysC at 2871.907 kb on - strand, within cysC at 2871.911 kb on - strand, within cysC at 2871.919 kb on + strand, within cysC at 2871.952 kb on + strand, within cysC at 2871.953 kb on - strand, within cysC at 2871.966 kb on - strand at 2871.968 kb on - strand at 2872.057 kb on - strand at 2872.264 kb on + strand, within cysN at 2872.298 kb on + strand, within cysN at 2872.478 kb on - strand, within cysN at 2872.760 kb on + strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.768 kb on - strand, within cysN at 2872.963 kb on + strand, within cysN at 2872.965 kb on + strand, within cysN at 2872.971 kb on - strand, within cysN at 2873.014 kb on + strand, within cysN at 2873.085 kb on - strand, within cysN at 2873.093 kb on + strand, within cysN at 2873.179 kb on - strand, within cysN at 2873.260 kb on + strand, within cysN at 2873.472 kb on + strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.480 kb on - strand at 2873.614 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.773 kb on + strand, within cysD at 2873.837 kb on + strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.951 kb on - strand, within cysD at 2873.953 kb on + strand, within cysD at 2874.048 kb on + strand, within cysD at 2874.225 kb on - strand, within cysD at 2874.306 kb on + strand at 2874.325 kb on - strand at 2874.330 kb on - strand at 2874.343 kb on - strand at 2874.362 kb on + strand at 2874.362 kb on + strand at 2874.367 kb on - strand at 2874.394 kb on - strand at 2874.394 kb on - strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.399 kb on + strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.407 kb on - strand at 2874.435 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,871,097 + ygbE b2749 0.19 +0.0 2,871,140 - ygbE b2749 0.32 -0.1 2,871,145 - ygbE b2749 0.34 -0.7 2,871,145 - ygbE b2749 0.34 +0.4 2,871,149 + ygbE b2749 0.35 +0.2 2,871,197 - ygbE b2749 0.50 +0.7 2,871,197 - ygbE b2749 0.50 +0.4 2,871,197 - ygbE b2749 0.50 +0.2 2,871,207 - ygbE b2749 0.53 +0.1 2,871,207 - ygbE b2749 0.53 -0.3 2,871,248 + ygbE b2749 0.65 -0.4 2,871,285 - ygbE b2749 0.77 -0.0 2,871,290 + ygbE b2749 0.78 -0.6 2,871,311 + ygbE b2749 0.85 +0.2 2,871,391 + -0.3 2,871,421 - +0.5 2,871,534 - cysC b2750 0.21 -0.2 2,871,692 - cysC b2750 0.47 -0.3 2,871,703 + cysC b2750 0.49 +0.5 2,871,807 - cysC b2750 0.66 -0.0 2,871,807 - cysC b2750 0.66 +0.1 2,871,866 + cysC b2750 0.75 +0.7 2,871,866 + cysC b2750 0.75 +0.3 2,871,907 - cysC b2750 0.82 +0.6 2,871,911 - cysC b2750 0.83 +0.2 2,871,919 + cysC b2750 0.84 -0.5 2,871,952 + cysC b2750 0.90 -0.9 2,871,953 - cysC b2750 0.90 -0.7 2,871,966 - +0.1 2,871,968 - +0.3 2,872,057 - -0.1 2,872,264 + cysN b2751 0.18 +0.3 2,872,298 + cysN b2751 0.20 -0.2 2,872,478 - cysN b2751 0.32 +0.0 2,872,760 + cysN b2751 0.52 -0.3 2,872,768 - cysN b2751 0.53 +0.2 2,872,768 - cysN b2751 0.53 +0.2 2,872,963 + cysN b2751 0.66 +0.4 2,872,965 + cysN b2751 0.67 +0.0 2,872,971 - cysN b2751 0.67 -1.0 2,873,014 + cysN b2751 0.70 +0.8 2,873,085 - cysN b2751 0.75 +0.2 2,873,093 + cysN b2751 0.76 +0.5 2,873,179 - cysN b2751 0.82 +0.2 2,873,260 + cysN b2751 0.87 +0.2 2,873,472 + +0.4 2,873,480 - +0.1 2,873,480 - -2.0 2,873,480 - -0.4 2,873,614 + cysD b2752 0.19 -0.1 2,873,773 + cysD b2752 0.36 -0.3 2,873,773 + cysD b2752 0.36 +0.6 2,873,837 + cysD b2752 0.43 +0.4 2,873,951 - cysD b2752 0.56 +0.4 2,873,951 - cysD b2752 0.56 +0.1 2,873,953 + cysD b2752 0.56 +0.5 2,874,048 + cysD b2752 0.67 +0.3 2,874,225 - cysD b2752 0.86 +0.0 2,874,306 + +0.2 2,874,325 - +0.1 2,874,330 - +0.1 2,874,343 - +0.1 2,874,362 + +0.3 2,874,362 + +0.6 2,874,367 - +0.0 2,874,394 - -0.0 2,874,394 - -0.5 2,874,399 + +0.2 2,874,399 + +0.7 2,874,399 + -0.2 2,874,399 + -0.6 2,874,407 - +0.2 2,874,407 - +0.2 2,874,407 - +0.3 2,874,435 + -1.4
Or see this region's nucleotide sequence