Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutL and eutC are separated by 9 nucleotides eutC and eutB are separated by 20 nucleotides eutB and intZ are separated by 178 nucleotides
b2439: eutL - predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI), at 2,553,763 to 2,554,422
eutL
b2440: eutC - ethanolamine ammonia-lyase small subunit (NCBI), at 2,554,432 to 2,555,319
eutC
b2441: eutB - ethanolamine ammonia-lyase, heavy chain (VIMSS), at 2,555,340 to 2,556,701
eutB
b2442: intZ - CPZ-55 prophage; predicted integrase (RefSeq), at 2,556,880 to 2,558,088
intZ
Position (kb)
2555
2556
2557 Strain fitness (log2 ratio)
-1
0
1 at 2554.436 kb on - strand at 2554.533 kb on - strand, within eutC at 2554.718 kb on + strand, within eutC at 2554.720 kb on + strand, within eutC at 2554.723 kb on - strand, within eutC at 2554.723 kb on - strand, within eutC at 2554.757 kb on - strand, within eutC at 2554.805 kb on + strand, within eutC at 2554.883 kb on + strand, within eutC at 2554.901 kb on - strand, within eutC at 2554.901 kb on - strand, within eutC at 2554.970 kb on + strand, within eutC at 2555.001 kb on + strand, within eutC at 2555.038 kb on - strand, within eutC at 2555.038 kb on - strand, within eutC at 2555.097 kb on + strand, within eutC at 2555.097 kb on + strand, within eutC at 2555.176 kb on + strand, within eutC at 2555.270 kb on + strand at 2555.270 kb on + strand at 2555.551 kb on - strand, within eutB at 2555.551 kb on - strand, within eutB at 2555.630 kb on - strand, within eutB at 2555.700 kb on - strand, within eutB at 2555.700 kb on - strand, within eutB at 2555.728 kb on + strand, within eutB at 2555.795 kb on - strand, within eutB at 2556.024 kb on + strand, within eutB at 2556.024 kb on + strand, within eutB at 2556.026 kb on + strand, within eutB at 2556.149 kb on + strand, within eutB at 2556.151 kb on - strand, within eutB at 2556.157 kb on - strand, within eutB at 2556.341 kb on + strand, within eutB at 2556.409 kb on - strand, within eutB at 2556.500 kb on - strand, within eutB at 2556.535 kb on - strand, within eutB at 2556.575 kb on + strand at 2556.663 kb on - strand at 2556.782 kb on + strand at 2556.782 kb on + strand at 2556.936 kb on + strand at 2557.047 kb on + strand, within intZ at 2557.094 kb on + strand, within intZ at 2557.144 kb on - strand, within intZ at 2557.406 kb on - strand, within intZ at 2557.475 kb on - strand, within intZ
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,554,436 - -0.1 2,554,533 - eutC b2440 0.11 -0.4 2,554,718 + eutC b2440 0.32 +0.2 2,554,720 + eutC b2440 0.32 +0.3 2,554,723 - eutC b2440 0.33 +0.0 2,554,723 - eutC b2440 0.33 +0.0 2,554,757 - eutC b2440 0.37 -0.5 2,554,805 + eutC b2440 0.42 +0.1 2,554,883 + eutC b2440 0.51 -0.2 2,554,901 - eutC b2440 0.53 -0.1 2,554,901 - eutC b2440 0.53 -0.9 2,554,970 + eutC b2440 0.61 +0.5 2,555,001 + eutC b2440 0.64 -0.2 2,555,038 - eutC b2440 0.68 +0.4 2,555,038 - eutC b2440 0.68 +0.3 2,555,097 + eutC b2440 0.75 +0.4 2,555,097 + eutC b2440 0.75 -0.1 2,555,176 + eutC b2440 0.84 -0.3 2,555,270 + +0.4 2,555,270 + -0.1 2,555,551 - eutB b2441 0.15 +0.5 2,555,551 - eutB b2441 0.15 -0.3 2,555,630 - eutB b2441 0.21 -0.4 2,555,700 - eutB b2441 0.26 -0.3 2,555,700 - eutB b2441 0.26 +0.4 2,555,728 + eutB b2441 0.28 -0.1 2,555,795 - eutB b2441 0.33 +0.3 2,556,024 + eutB b2441 0.50 +0.2 2,556,024 + eutB b2441 0.50 -0.0 2,556,026 + eutB b2441 0.50 -0.9 2,556,149 + eutB b2441 0.59 +0.1 2,556,151 - eutB b2441 0.60 -0.7 2,556,157 - eutB b2441 0.60 +0.2 2,556,341 + eutB b2441 0.73 -0.2 2,556,409 - eutB b2441 0.78 +0.2 2,556,500 - eutB b2441 0.85 +0.5 2,556,535 - eutB b2441 0.88 +0.3 2,556,575 + +0.2 2,556,663 - +0.4 2,556,782 + +0.3 2,556,782 + +0.4 2,556,936 + +0.2 2,557,047 + intZ b2442 0.14 +0.1 2,557,094 + intZ b2442 0.18 +0.2 2,557,144 - intZ b2442 0.22 +0.8 2,557,406 - intZ b2442 0.44 -0.3 2,557,475 - intZ b2442 0.49 +0.1
Or see this region's nucleotide sequence