Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pabB and yeaB are separated by 3 nucleotides yeaB and sdaA are separated by 183 nucleotides sdaA and b1815 are separated by 130 nucleotides
b1812: pabB - para-aminobenzoate synthase component I (NCBI), at 1,892,829 to 1,894,190
pabB
b1813: yeaB - predicted NUDIX hydrolase (NCBI), at 1,894,194 to 1,894,772
yeaB
b1814: sdaA - L-serine deaminase I (NCBI), at 1,894,956 to 1,896,320
sdaA
b1815: b1815 - orf, hypothetical protein (VIMSS), at 1,896,451 to 1,898,049
b1815
Position (kb)
1894
1895
1896
1897 Strain fitness (log2 ratio)
-1
0
1 at 1893.960 kb on - strand, within pabB at 1893.960 kb on - strand, within pabB at 1894.035 kb on + strand, within pabB at 1894.301 kb on - strand, within yeaB at 1894.400 kb on + strand, within yeaB at 1894.406 kb on - strand, within yeaB at 1894.419 kb on + strand, within yeaB at 1894.446 kb on + strand, within yeaB at 1894.456 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.500 kb on + strand, within yeaB at 1894.551 kb on - strand, within yeaB at 1894.579 kb on - strand, within yeaB at 1894.629 kb on - strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.683 kb on + strand, within yeaB at 1894.686 kb on - strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.693 kb on + strand, within yeaB at 1894.735 kb on + strand at 1894.766 kb on + strand at 1894.766 kb on + strand at 1894.804 kb on + strand at 1894.875 kb on - strand at 1894.875 kb on - strand at 1894.890 kb on + strand at 1894.890 kb on + strand at 1894.915 kb on - strand at 1894.961 kb on - strand at 1894.997 kb on + strand at 1895.002 kb on - strand at 1895.028 kb on - strand at 1895.028 kb on - strand at 1895.039 kb on - strand at 1895.039 kb on - strand at 1895.465 kb on + strand, within sdaA at 1895.498 kb on - strand, within sdaA at 1895.540 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA at 1895.646 kb on - strand, within sdaA at 1896.004 kb on - strand, within sdaA at 1896.004 kb on - strand, within sdaA at 1896.254 kb on + strand at 1896.386 kb on - strand at 1896.440 kb on - strand at 1896.440 kb on - strand at 1896.564 kb on + strand at 1896.618 kb on - strand, within b1815 at 1896.650 kb on + strand, within b1815 at 1896.681 kb on + strand, within b1815 at 1896.681 kb on + strand, within b1815 at 1896.686 kb on + strand, within b1815 at 1896.770 kb on - strand, within b1815 at 1896.770 kb on - strand, within b1815 at 1896.929 kb on + strand, within b1815 at 1896.956 kb on - strand, within b1815 at 1897.151 kb on - strand, within b1815
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 1,893,960 - pabB b1812 0.83 +0.5 1,893,960 - pabB b1812 0.83 +0.3 1,894,035 + pabB b1812 0.89 +0.9 1,894,301 - yeaB b1813 0.18 +0.7 1,894,400 + yeaB b1813 0.36 +0.1 1,894,406 - yeaB b1813 0.37 +0.4 1,894,419 + yeaB b1813 0.39 +0.1 1,894,446 + yeaB b1813 0.44 +0.7 1,894,456 + yeaB b1813 0.45 +0.1 1,894,500 + yeaB b1813 0.53 +0.1 1,894,500 + yeaB b1813 0.53 -0.8 1,894,551 - yeaB b1813 0.62 +0.4 1,894,579 - yeaB b1813 0.66 +0.4 1,894,629 - yeaB b1813 0.75 -0.4 1,894,683 + yeaB b1813 0.84 +0.1 1,894,683 + yeaB b1813 0.84 +0.5 1,894,686 - yeaB b1813 0.85 +0.1 1,894,693 + yeaB b1813 0.86 -0.8 1,894,693 + yeaB b1813 0.86 -0.5 1,894,735 + +0.0 1,894,766 + +0.2 1,894,766 + -0.0 1,894,804 + +0.3 1,894,875 - -1.6 1,894,875 - +0.6 1,894,890 + +0.4 1,894,890 + +0.6 1,894,915 - -0.5 1,894,961 - +0.1 1,894,997 + -1.2 1,895,002 - -1.0 1,895,028 - +0.4 1,895,028 - +0.1 1,895,039 - -0.8 1,895,039 - -0.4 1,895,465 + sdaA b1814 0.37 -0.3 1,895,498 - sdaA b1814 0.40 -1.4 1,895,540 - sdaA b1814 0.43 -0.4 1,895,646 - sdaA b1814 0.51 -0.3 1,895,646 - sdaA b1814 0.51 -1.2 1,896,004 - sdaA b1814 0.77 -1.6 1,896,004 - sdaA b1814 0.77 -0.9 1,896,254 + +0.1 1,896,386 - -0.2 1,896,440 - +0.0 1,896,440 - +0.2 1,896,564 + -0.4 1,896,618 - b1815 b1815 0.10 +0.1 1,896,650 + b1815 b1815 0.12 +0.3 1,896,681 + b1815 b1815 0.14 +0.4 1,896,681 + b1815 b1815 0.14 +0.4 1,896,686 + b1815 b1815 0.15 +0.4 1,896,770 - b1815 b1815 0.20 +0.1 1,896,770 - b1815 b1815 0.20 +0.3 1,896,929 + b1815 b1815 0.30 +0.3 1,896,956 - b1815 b1815 0.32 +0.0 1,897,151 - b1815 b1815 0.44 +0.3
Or see this region's nucleotide sequence