Strain Fitness in Escherichia coli BW25113 around b1612

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfumC and fumA are separated by 142 nucleotidesfumA and manA are separated by 198 nucleotides b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612 fumC b1612: fumA - fumarate hydratase (fumarase A), aerobic Class I (NCBI), at 1,684,755 to 1,686,401 fumA b1613: manA - mannose-6-phosphate isomerase (VIMSS), at 1,686,600 to 1,687,775 manA Position (kb) 1684 1685 1686 1687Strain fitness (log2 ratio) -1 0 1at 1683.859 kb on - strand, within fumCat 1683.859 kb on - strand, within fumCat 1683.981 kb on - strand, within fumCat 1684.016 kb on - strand, within fumCat 1684.016 kb on - strand, within fumCat 1684.247 kb on + strand, within fumCat 1684.247 kb on + strand, within fumCat 1684.600 kb on + strandat 1684.600 kb on + strandat 1685.163 kb on + strand, within fumAat 1685.193 kb on + strand, within fumAat 1685.290 kb on - strand, within fumAat 1685.290 kb on - strand, within fumAat 1685.310 kb on + strand, within fumAat 1685.344 kb on + strand, within fumAat 1685.895 kb on - strand, within fumAat 1685.895 kb on - strand, within fumAat 1686.443 kb on + strandat 1686.629 kb on - strandat 1686.693 kb on + strandat 1686.718 kb on - strand, within manAat 1686.749 kb on - strand, within manAat 1686.749 kb on - strand, within manAat 1686.883 kb on - strand, within manAat 1687.299 kb on + strand, within manAat 1687.351 kb on + strand, within manA

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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1,683,859 - fumC b1611 0.46 +0.1
1,683,859 - fumC b1611 0.46 -1.0
1,683,981 - fumC b1611 0.55 +0.1
1,684,016 - fumC b1611 0.57 +0.3
1,684,016 - fumC b1611 0.57 +0.3
1,684,247 + fumC b1611 0.74 +0.5
1,684,247 + fumC b1611 0.74 -0.4
1,684,600 + -0.2
1,684,600 + +0.0
1,685,163 + fumA b1612 0.25 -0.2
1,685,193 + fumA b1612 0.27 +0.0
1,685,290 - fumA b1612 0.32 -0.5
1,685,290 - fumA b1612 0.32 -0.1
1,685,310 + fumA b1612 0.34 -0.4
1,685,344 + fumA b1612 0.36 +1.0
1,685,895 - fumA b1612 0.69 +0.8
1,685,895 - fumA b1612 0.69 +0.6
1,686,443 + +0.4
1,686,629 - +0.4
1,686,693 + +0.3
1,686,718 - manA b1613 0.10 -1.2
1,686,749 - manA b1613 0.13 -1.1
1,686,749 - manA b1613 0.13 +0.6
1,686,883 - manA b1613 0.24 -0.2
1,687,299 + manA b1613 0.59 +0.1
1,687,351 + manA b1613 0.64 -0.1

Or see this region's nucleotide sequence