Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabF and pabC are separated by 119 nucleotides pabC and yceG are separated by 2 nucleotides yceG and tmk overlap by 11 nucleotides tmk and holB overlap by 4 nucleotides
b1095: fabF - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,151,162 to 1,152,403
fabF
b1096: pabC - 4-amino-4-deoxychorismate lyase (NCBI), at 1,152,523 to 1,153,332
pabC
b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357
yceG
b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988
tmk
b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989
holB
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-1
0
1 at 1152.335 kb on + strand at 1152.361 kb on - strand at 1152.361 kb on - strand at 1152.420 kb on + strand at 1152.420 kb on + strand at 1152.474 kb on - strand at 1152.482 kb on - strand at 1152.520 kb on + strand at 1152.520 kb on + strand at 1152.558 kb on + strand at 1152.676 kb on - strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.788 kb on + strand, within pabC at 1152.876 kb on + strand, within pabC at 1152.902 kb on - strand, within pabC at 1152.909 kb on + strand, within pabC at 1152.909 kb on + strand, within pabC at 1153.012 kb on + strand, within pabC at 1153.171 kb on + strand, within pabC at 1153.177 kb on + strand, within pabC at 1153.208 kb on - strand, within pabC at 1153.223 kb on + strand, within pabC at 1153.301 kb on - strand at 1153.412 kb on + strand at 1153.412 kb on + strand at 1153.585 kb on - strand, within yceG at 1153.597 kb on + strand, within yceG at 1153.603 kb on - strand, within yceG at 1153.609 kb on + strand, within yceG at 1153.656 kb on + strand, within yceG at 1153.697 kb on - strand, within yceG at 1153.802 kb on + strand, within yceG at 1153.907 kb on + strand, within yceG at 1153.907 kb on + strand, within yceG at 1153.934 kb on + strand, within yceG at 1154.104 kb on - strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.150 kb on + strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.152 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.158 kb on - strand, within yceG at 1154.160 kb on - strand, within yceG at 1154.280 kb on - strand at 1154.323 kb on + strand at 1154.323 kb on + strand at 1155.056 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 1,152,335 + -1.5 1,152,361 - -1.6 1,152,361 - +0.0 1,152,420 + -0.0 1,152,420 + +0.2 1,152,474 - -0.5 1,152,482 - +0.0 1,152,520 + +0.2 1,152,520 + -0.6 1,152,558 + +1.0 1,152,676 - pabC b1096 0.19 +0.3 1,152,788 + pabC b1096 0.33 +0.3 1,152,788 + pabC b1096 0.33 +0.3 1,152,876 + pabC b1096 0.44 +0.6 1,152,902 - pabC b1096 0.47 -0.7 1,152,909 + pabC b1096 0.48 -0.2 1,152,909 + pabC b1096 0.48 +0.0 1,153,012 + pabC b1096 0.60 -0.1 1,153,171 + pabC b1096 0.80 -0.1 1,153,177 + pabC b1096 0.81 +0.2 1,153,208 - pabC b1096 0.85 +0.2 1,153,223 + pabC b1096 0.86 -0.8 1,153,301 - +0.0 1,153,412 + -1.1 1,153,412 + -0.8 1,153,585 - yceG b1097 0.24 +0.9 1,153,597 + yceG b1097 0.26 -0.9 1,153,603 - yceG b1097 0.26 -0.9 1,153,609 + yceG b1097 0.27 -0.8 1,153,656 + yceG b1097 0.31 -0.7 1,153,697 - yceG b1097 0.35 -1.4 1,153,802 + yceG b1097 0.46 -1.1 1,153,907 + yceG b1097 0.56 -0.2 1,153,907 + yceG b1097 0.56 -1.3 1,153,934 + yceG b1097 0.59 -1.1 1,154,104 - yceG b1097 0.75 -0.9 1,154,150 + yceG b1097 0.80 -0.9 1,154,150 + yceG b1097 0.80 -1.1 1,154,152 - yceG b1097 0.80 -0.5 1,154,152 - yceG b1097 0.80 -0.8 1,154,158 - yceG b1097 0.80 -0.7 1,154,158 - yceG b1097 0.80 -1.6 1,154,160 - yceG b1097 0.81 -1.4 1,154,280 - -0.7 1,154,323 + -0.3 1,154,323 + +0.4 1,155,056 + -0.2
Or see this region's nucleotide sequence