Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucB and sucC are separated by 274 nucleotides sucC and sucD overlap by 1 nucleotides sucD and mngR are separated by 103 nucleotides
b0727: sucB - dihydrolipoamide acetyltransferase (NCBI), at 760,745 to 761,962
sucB
b0728: sucC - succinyl-CoA synthetase subunit beta (NCBI), at 762,237 to 763,403
sucC
b0729: sucD - succinyl-CoA synthetase subunit alpha (NCBI), at 763,403 to 764,272
sucD
b0730: mngR - DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI), at 764,376 to 765,098
mngR
Position (kb) 762
763
764 Strain fitness (log2 ratio) -1
0
1
2 at 761.505 kb on + strand, within sucB at 761.728 kb on + strand, within sucB at 761.860 kb on + strand at 761.970 kb on + strand at 761.992 kb on + strand at 762.001 kb on - strand at 762.146 kb on + strand at 762.154 kb on - strand at 762.154 kb on - strand at 762.196 kb on + strand at 762.223 kb on + strand at 762.284 kb on - strand at 762.308 kb on - strand at 762.310 kb on + strand at 762.318 kb on - strand at 762.398 kb on - strand, within sucC at 762.398 kb on - strand, within sucC at 762.600 kb on - strand, within sucC at 762.708 kb on + strand, within sucC at 762.708 kb on + strand, within sucC at 762.713 kb on + strand, within sucC at 762.989 kb on - strand, within sucC at 762.999 kb on - strand, within sucC at 763.015 kb on + strand, within sucC at 763.070 kb on - strand, within sucC at 763.131 kb on + strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.206 kb on - strand, within sucC at 763.278 kb on + strand, within sucC at 763.625 kb on + strand, within sucD at 763.625 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.712 kb on + strand, within sucD at 763.773 kb on + strand, within sucD at 763.871 kb on - strand, within sucD at 763.901 kb on + strand, within sucD at 764.252 kb on - strand at 764.279 kb on + strand at 764.302 kb on + strand at 764.302 kb on + strand at 764.311 kb on + strand at 764.319 kb on + strand at 764.349 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 761,505 + sucB b0727 0.62 +1.0 761,728 + sucB b0727 0.81 +0.1 761,860 + -0.8 761,970 + -0.1 761,992 + -0.3 762,001 - -0.3 762,146 + +0.1 762,154 - +0.2 762,154 - +0.4 762,196 + +0.2 762,223 + +0.5 762,284 - -0.1 762,308 - +0.8 762,310 + +0.2 762,318 - +0.2 762,398 - sucC b0728 0.14 -0.7 762,398 - sucC b0728 0.14 -0.1 762,600 - sucC b0728 0.31 +0.3 762,708 + sucC b0728 0.40 +0.1 762,708 + sucC b0728 0.40 -0.2 762,713 + sucC b0728 0.41 -0.2 762,989 - sucC b0728 0.64 +0.0 762,999 - sucC b0728 0.65 -0.1 763,015 + sucC b0728 0.67 +0.0 763,070 - sucC b0728 0.71 +0.7 763,131 + sucC b0728 0.77 -0.2 763,206 - sucC b0728 0.83 -0.1 763,206 - sucC b0728 0.83 +0.3 763,206 - sucC b0728 0.83 +1.0 763,278 + sucC b0728 0.89 +0.2 763,625 + sucD b0729 0.26 -0.1 763,625 + sucD b0729 0.26 +0.2 763,712 + sucD b0729 0.36 +1.8 763,712 + sucD b0729 0.36 +0.3 763,773 + sucD b0729 0.43 +0.5 763,871 - sucD b0729 0.54 +0.1 763,901 + sucD b0729 0.57 -0.6 764,252 - +0.3 764,279 + -0.8 764,302 + -0.7 764,302 + +0.1 764,311 + +0.5 764,319 + -0.2 764,349 + +0.1
Or see this region's nucleotide sequence