Strain Fitness in Escherichia coli BW25113 around b0682

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybfM and ybfN are separated by 49 nucleotidesybfN and fur are separated by 83 nucleotidesfur and uof overlap by 8 nucleotidesuof and fldA are separated by 209 nucleotides b0681: ybfM - predicted outer membrane porin (NCBI), at 707,557 to 708,963 ybfM b0682: ybfN - predicted lipoprotein (NCBI), at 709,013 to 709,339 ybfN b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869 fur b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948 uof b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688 fldA Position (kb) 709 710Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 708.126 kb on + strand, within ybfMat 708.126 kb on + strand, within ybfMat 708.224 kb on + strand, within ybfMat 708.279 kb on - strand, within ybfMat 708.288 kb on + strand, within ybfMat 708.288 kb on + strand, within ybfMat 708.288 kb on + strand, within ybfMat 708.288 kb on + strand, within ybfMat 708.291 kb on + strand, within ybfMat 708.389 kb on - strand, within ybfMat 708.389 kb on - strand, within ybfMat 708.760 kb on - strand, within ybfMat 708.764 kb on + strand, within ybfMat 708.766 kb on + strand, within ybfMat 708.766 kb on + strand, within ybfMat 708.785 kb on + strand, within ybfMat 708.785 kb on + strand, within ybfMat 708.800 kb on + strand, within ybfMat 708.803 kb on + strand, within ybfMat 708.894 kb on - strandat 708.943 kb on + strandat 709.039 kb on + strandat 709.113 kb on - strand, within ybfNat 709.113 kb on - strand, within ybfNat 709.126 kb on - strand, within ybfNat 709.238 kb on + strand, within ybfNat 709.247 kb on - strand, within ybfNat 709.247 kb on - strand, within ybfNat 709.257 kb on - strand, within ybfNat 709.428 kb on - strandat 709.428 kb on - strandat 709.595 kb on + strand, within furat 709.678 kb on - strand, within furat 709.699 kb on + strand, within furat 709.861 kb on - strandat 710.067 kb on - strandat 710.080 kb on + strandat 710.148 kb on - strandat 710.148 kb on - strandat 710.163 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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708,126 + ybfM b0681 0.40 -0.5
708,126 + ybfM b0681 0.40 +0.1
708,224 + ybfM b0681 0.47 -0.3
708,279 - ybfM b0681 0.51 +0.3
708,288 + ybfM b0681 0.52 +0.2
708,288 + ybfM b0681 0.52 -0.9
708,288 + ybfM b0681 0.52 -0.4
708,288 + ybfM b0681 0.52 +0.1
708,291 + ybfM b0681 0.52 +0.1
708,389 - ybfM b0681 0.59 -0.3
708,389 - ybfM b0681 0.59 +0.7
708,760 - ybfM b0681 0.86 -0.1
708,764 + ybfM b0681 0.86 +0.2
708,766 + ybfM b0681 0.86 -0.2
708,766 + ybfM b0681 0.86 +0.0
708,785 + ybfM b0681 0.87 +0.1
708,785 + ybfM b0681 0.87 +0.4
708,800 + ybfM b0681 0.88 +0.6
708,803 + ybfM b0681 0.89 +0.1
708,894 - -0.1
708,943 + -0.4
709,039 + -0.1
709,113 - ybfN b0682 0.31 +0.1
709,113 - ybfN b0682 0.31 -0.0
709,126 - ybfN b0682 0.35 -0.4
709,238 + ybfN b0682 0.69 -0.2
709,247 - ybfN b0682 0.72 +0.3
709,247 - ybfN b0682 0.72 +0.2
709,257 - ybfN b0682 0.75 -0.1
709,428 - +0.0
709,428 - +0.1
709,595 + fur b0683 0.38 -3.6
709,678 - fur b0683 0.57 -2.1
709,699 + fur b0683 0.62 -3.9
709,861 - +0.4
710,067 - +0.7
710,080 + -0.1
710,148 - +0.7
710,148 - -0.1
710,163 - +0.3

Or see this region's nucleotide sequence