Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepA and fes are separated by 320 nucleotides fes and ybdZ are separated by 2 nucleotides ybdZ and entF overlap by 4 nucleotides
b0584: fepA - iron-enterobactin outer membrane transporter (NCBI), at 609,477 to 611,717
fepA
b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162
fes
b4511: ybdZ - hypothetical protein (NCBI), at 613,165 to 613,383
ybdZ
b0586: entF - enterobactin synthase multienzyme complex component, ATP-dependent (NCBI), at 613,380 to 617,261
entF
Position (kb) 612
613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 611.112 kb on + strand, within fepA at 611.148 kb on - strand, within fepA at 611.188 kb on - strand, within fepA at 611.322 kb on + strand, within fepA at 611.500 kb on + strand at 611.500 kb on + strand at 611.500 kb on + strand at 612.036 kb on - strand at 612.036 kb on - strand at 612.112 kb on + strand at 612.112 kb on + strand at 612.117 kb on - strand at 612.132 kb on + strand at 612.132 kb on + strand at 612.203 kb on - strand, within fes at 612.203 kb on - strand, within fes at 612.234 kb on + strand, within fes at 612.272 kb on - strand, within fes at 612.424 kb on + strand, within fes at 612.424 kb on + strand, within fes at 612.629 kb on - strand, within fes at 612.629 kb on - strand, within fes at 612.676 kb on - strand, within fes at 612.835 kb on + strand, within fes at 612.840 kb on + strand, within fes at 612.843 kb on - strand, within fes at 612.843 kb on - strand, within fes at 612.868 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.869 kb on - strand, within fes at 612.871 kb on + strand, within fes at 612.883 kb on + strand, within fes at 612.960 kb on - strand, within fes at 613.018 kb on + strand, within fes at 613.018 kb on + strand, within fes at 613.181 kb on - strand at 613.181 kb on - strand at 613.203 kb on + strand, within ybdZ at 613.270 kb on + strand, within ybdZ at 613.340 kb on + strand, within ybdZ at 613.396 kb on - strand at 613.455 kb on + strand at 613.455 kb on + strand at 613.523 kb on + strand at 613.523 kb on + strand at 613.549 kb on - strand at 613.549 kb on - strand at 613.589 kb on + strand at 613.643 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.663 kb on + strand at 613.671 kb on - strand at 613.671 kb on - strand at 613.870 kb on - strand, within entF at 613.923 kb on + strand, within entF at 613.923 kb on + strand, within entF at 613.927 kb on - strand, within entF at 613.974 kb on + strand, within entF at 614.028 kb on - strand, within entF at 614.050 kb on - strand, within entF at 614.050 kb on - strand, within entF
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 611,112 + fepA b0584 0.73 +0.7 611,148 - fepA b0584 0.75 +0.1 611,188 - fepA b0584 0.76 +0.5 611,322 + fepA b0584 0.82 -0.6 611,500 + +0.7 611,500 + +0.5 611,500 + +1.3 612,036 - -0.3 612,036 - -0.2 612,112 + -0.6 612,112 + -2.1 612,117 - -1.2 612,132 + -0.8 612,132 + -1.1 612,203 - fes b0585 0.15 -0.5 612,203 - fes b0585 0.15 -0.3 612,234 + fes b0585 0.17 -0.8 612,272 - fes b0585 0.21 -2.9 612,424 + fes b0585 0.34 -0.9 612,424 + fes b0585 0.34 -0.4 612,629 - fes b0585 0.53 -1.9 612,629 - fes b0585 0.53 -0.4 612,676 - fes b0585 0.57 -0.8 612,835 + fes b0585 0.71 -0.5 612,840 + fes b0585 0.71 -0.5 612,843 - fes b0585 0.72 -0.7 612,843 - fes b0585 0.72 -1.3 612,868 - fes b0585 0.74 -1.9 612,869 - fes b0585 0.74 -0.0 612,869 - fes b0585 0.74 -1.4 612,871 + fes b0585 0.74 -0.0 612,883 + fes b0585 0.75 +0.0 612,960 - fes b0585 0.82 -0.9 613,018 + fes b0585 0.87 -0.5 613,018 + fes b0585 0.87 -0.6 613,181 - -0.1 613,181 - -0.3 613,203 + ybdZ b4511 0.17 +0.0 613,270 + ybdZ b4511 0.48 +0.3 613,340 + ybdZ b4511 0.80 +0.2 613,396 - +0.2 613,455 + +0.5 613,455 + -0.1 613,523 + +1.2 613,523 + +0.4 613,549 - +0.2 613,549 - +0.2 613,589 + +0.5 613,643 + +1.0 613,663 + +0.4 613,663 + -0.5 613,663 + +0.4 613,671 - +0.3 613,671 - +0.2 613,870 - entF b0586 0.13 +0.6 613,923 + entF b0586 0.14 -0.7 613,923 + entF b0586 0.14 +0.9 613,927 - entF b0586 0.14 +0.2 613,974 + entF b0586 0.15 +0.5 614,028 - entF b0586 0.17 +0.1 614,050 - entF b0586 0.17 -0.3 614,050 - entF b0586 0.17 +0.5
Or see this region's nucleotide sequence