Strain Fitness in Escherichia coli BW25113 around b0185

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdnaE and accA are separated by 12 nucleotidesaccA and ldcC are separated by 98 nucleotides b0184: dnaE - DNA polymerase III subunit alpha (NCBI), at 205,126 to 208,608 dnaE b0185: accA - acetyl-CoA carboxylase subunit alpha (NCBI), at 208,621 to 209,580 accA b0186: ldcC - lysine decarboxylase 2, constitutive (NCBI), at 209,679 to 211,820 ldcC Position (kb) 208 209 210Strain fitness (log2 ratio) -1 0 1at 208.591 kb on + strandat 209.655 kb on - strandat 209.826 kb on - strandat 209.832 kb on - strandat 209.856 kb on + strandat 209.856 kb on + strandat 209.864 kb on - strandat 209.930 kb on - strand, within ldcCat 209.944 kb on - strand, within ldcCat 209.960 kb on + strand, within ldcCat 209.960 kb on + strand, within ldcCat 209.961 kb on - strand, within ldcCat 209.994 kb on + strand, within ldcCat 209.997 kb on - strand, within ldcCat 210.032 kb on + strand, within ldcCat 210.098 kb on - strand, within ldcCat 210.202 kb on + strand, within ldcCat 210.251 kb on + strand, within ldcCat 210.375 kb on + strand, within ldcCat 210.375 kb on + strand, within ldcCat 210.398 kb on - strand, within ldcCat 210.425 kb on - strand, within ldcCat 210.440 kb on + strand, within ldcCat 210.458 kb on - strand, within ldcCat 210.549 kb on + strand, within ldcCat 210.557 kb on - strand, within ldcC

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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208,591 + +0.0
209,655 - +0.5
209,826 - +0.2
209,832 - +0.5
209,856 + -0.1
209,856 + -0.0
209,864 - +0.3
209,930 - ldcC b0186 0.12 -0.8
209,944 - ldcC b0186 0.12 +0.0
209,960 + ldcC b0186 0.13 -0.8
209,960 + ldcC b0186 0.13 +0.9
209,961 - ldcC b0186 0.13 +0.2
209,994 + ldcC b0186 0.15 -0.0
209,997 - ldcC b0186 0.15 +0.3
210,032 + ldcC b0186 0.16 +1.1
210,098 - ldcC b0186 0.20 -0.4
210,202 + ldcC b0186 0.24 -0.2
210,251 + ldcC b0186 0.27 -0.2
210,375 + ldcC b0186 0.32 -0.7
210,375 + ldcC b0186 0.32 +0.8
210,398 - ldcC b0186 0.34 -0.1
210,425 - ldcC b0186 0.35 +0.8
210,440 + ldcC b0186 0.36 +0.2
210,458 - ldcC b0186 0.36 +0.3
210,549 + ldcC b0186 0.41 -0.2
210,557 - ldcC b0186 0.41 +0.1

Or see this region's nucleotide sequence