Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadN and folK are separated by 369 nucleotides folK and pcnB overlap by 4 nucleotides pcnB and yadB are separated by 59 nucleotides
b0141: yadN - predicted fimbrial-like adhesin protein (NCBI), at 156,299 to 156,883
yadN
b0142: folK - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase (NCBI), at 157,253 to 157,732
folK
b0143: pcnB - poly(A) polymerase I (VIMSS), at 157,729 to 159,126
pcnB
b0144: yadB - glutamyl-Q tRNA(Asp) synthetase (RefSeq), at 159,186 to 160,112
yadB
Position (kb)
157
158
159
160 Strain fitness (log2 ratio)
-1
0
1 at 156.734 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.735 kb on + strand, within yadN at 156.744 kb on + strand, within yadN at 156.755 kb on + strand, within yadN at 156.757 kb on + strand, within yadN at 156.759 kb on + strand, within yadN at 156.786 kb on + strand, within yadN at 156.789 kb on + strand, within yadN at 156.793 kb on + strand, within yadN at 156.804 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.823 kb on + strand, within yadN at 156.825 kb on + strand at 156.827 kb on + strand at 156.827 kb on + strand at 156.834 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.849 kb on + strand at 156.850 kb on + strand at 156.854 kb on + strand at 156.854 kb on + strand at 157.218 kb on - strand at 157.279 kb on - strand at 157.291 kb on - strand at 157.301 kb on - strand, within folK at 157.745 kb on + strand at 157.870 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.906 kb on - strand, within pcnB at 157.923 kb on - strand, within pcnB at 158.143 kb on + strand, within pcnB at 158.156 kb on + strand, within pcnB at 158.227 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.249 kb on - strand, within pcnB at 158.312 kb on - strand, within pcnB at 158.342 kb on - strand, within pcnB at 158.378 kb on - strand, within pcnB at 158.400 kb on + strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.408 kb on - strand, within pcnB at 158.426 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.442 kb on - strand, within pcnB at 158.447 kb on - strand, within pcnB at 158.516 kb on + strand, within pcnB at 158.522 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.759 kb on - strand, within pcnB at 158.864 kb on - strand, within pcnB at 159.038 kb on - strand at 159.093 kb on - strand at 159.093 kb on - strand at 159.133 kb on + strand at 159.347 kb on - strand, within yadB at 159.347 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.352 kb on - strand, within yadB at 159.581 kb on - strand, within yadB at 159.655 kb on + strand, within yadB at 159.699 kb on + strand, within yadB at 159.722 kb on - strand, within yadB at 159.722 kb on - strand, within yadB at 159.743 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.754 kb on - strand, within yadB at 159.770 kb on + strand, within yadB at 159.770 kb on + strand, within yadB at 159.803 kb on + strand, within yadB at 159.808 kb on + strand, within yadB at 159.905 kb on - strand, within yadB at 159.947 kb on + strand, within yadB at 160.046 kb on + strand at 160.068 kb on + strand at 160.068 kb on - strand at 160.068 kb on - strand at 160.070 kb on - strand at 160.078 kb on + strand at 160.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 156,734 + yadN b0141 0.74 +0.1 156,735 + yadN b0141 0.75 -0.8 156,735 + yadN b0141 0.75 +0.7 156,744 + yadN b0141 0.76 +0.9 156,755 + yadN b0141 0.78 -0.2 156,757 + yadN b0141 0.78 -0.5 156,759 + yadN b0141 0.79 +0.1 156,786 + yadN b0141 0.83 -0.7 156,789 + yadN b0141 0.84 -0.0 156,793 + yadN b0141 0.84 +0.5 156,804 + yadN b0141 0.86 +0.3 156,823 + yadN b0141 0.90 -0.0 156,823 + yadN b0141 0.90 +0.1 156,825 + +0.0 156,827 + +0.3 156,827 + +0.9 156,834 + -0.6 156,849 + -0.4 156,849 + -0.1 156,849 + +0.3 156,850 + +0.1 156,854 + +0.8 156,854 + +0.3 157,218 - -0.1 157,279 - -0.3 157,291 - -0.1 157,301 - folK b0142 0.10 -0.1 157,745 + -0.6 157,870 - pcnB b0143 0.10 +0.2 157,906 - pcnB b0143 0.13 -1.3 157,906 - pcnB b0143 0.13 -0.1 157,923 - pcnB b0143 0.14 -1.0 158,143 + pcnB b0143 0.30 -1.6 158,156 + pcnB b0143 0.31 -0.0 158,227 - pcnB b0143 0.36 -0.5 158,249 - pcnB b0143 0.37 -0.7 158,249 - pcnB b0143 0.37 -0.2 158,312 - pcnB b0143 0.42 -0.9 158,342 - pcnB b0143 0.44 -0.5 158,378 - pcnB b0143 0.46 -1.0 158,400 + pcnB b0143 0.48 -0.8 158,408 - pcnB b0143 0.49 -0.6 158,408 - pcnB b0143 0.49 -0.6 158,426 - pcnB b0143 0.50 -1.5 158,442 - pcnB b0143 0.51 -1.2 158,442 - pcnB b0143 0.51 +0.5 158,447 - pcnB b0143 0.51 -1.0 158,516 + pcnB b0143 0.56 -1.3 158,522 - pcnB b0143 0.57 -0.7 158,759 - pcnB b0143 0.74 -0.7 158,759 - pcnB b0143 0.74 +1.0 158,759 - pcnB b0143 0.74 -0.5 158,864 - pcnB b0143 0.81 -0.3 159,038 - -0.4 159,093 - +1.3 159,093 - -0.3 159,133 + -1.1 159,347 - yadB b0144 0.17 +0.7 159,347 - yadB b0144 0.17 -0.2 159,352 - yadB b0144 0.18 +0.2 159,352 - yadB b0144 0.18 -0.1 159,581 - yadB b0144 0.43 +0.0 159,655 + yadB b0144 0.51 -0.1 159,699 + yadB b0144 0.55 +0.6 159,722 - yadB b0144 0.58 +0.6 159,722 - yadB b0144 0.58 +0.2 159,743 - yadB b0144 0.60 -0.3 159,754 - yadB b0144 0.61 -0.6 159,754 - yadB b0144 0.61 +0.6 159,770 + yadB b0144 0.63 +0.1 159,770 + yadB b0144 0.63 +0.2 159,803 + yadB b0144 0.67 +0.3 159,808 + yadB b0144 0.67 +0.2 159,905 - yadB b0144 0.78 +0.5 159,947 + yadB b0144 0.82 -0.2 160,046 + +0.2 160,068 + +0.3 160,068 - -1.1 160,068 - +0.1 160,070 - +0.6 160,078 + +0.8 160,078 + -0.2
Or see this region's nucleotide sequence