Strain Fitness in Escherichia coli BW25113 around b0115

Experiment: D-Cycloserine 0.1 mg/ml

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntaceE and aceF are separated by 14 nucleotidesaceF and lpd are separated by 324 nucleotides b0114: aceE - pyruvate dehydrogenase subunit E1 (NCBI), at 123,017 to 125,680 aceE b0115: aceF - dihydrolipoamide acetyltransferase (NCBI), at 125,695 to 127,587 aceF b0116: lpd - dihydrolipoamide dehydrogenase (NCBI), at 127,912 to 129,336 lpd Position (kb) 125 126 127 128Strain fitness (log2 ratio) -1 0 1at 124.820 kb on + strand, within aceEat 124.849 kb on + strand, within aceEat 125.049 kb on - strand, within aceEat 125.327 kb on - strand, within aceEat 127.735 kb on + strandat 127.735 kb on + strandat 127.768 kb on + strandat 127.893 kb on + strandat 127.898 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
remove
124,820 + aceE b0114 0.68 -0.0
124,849 + aceE b0114 0.69 -0.5
125,049 - aceE b0114 0.76 -0.9
125,327 - aceE b0114 0.87 -1.2
127,735 + -0.1
127,735 + +0.6
127,768 + -0.4
127,893 + +0.5
127,898 - -0.7

Or see this region's nucleotide sequence