Strain Fitness in Escherichia coli BW25113 around b4104

Experiment: Phosphomycin 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntphnG and phnF are separated by 0 nucleotidesphnF and phnE are separated by 20 nucleotidesphnE and phnD are separated by 521 nucleotides b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719 phnG b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445 phnF b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837 phnE b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375 phnD Position (kb) 4320 4321Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8 9 10 11 12 13at 4319.573 kb on + strand, within phnGat 4319.731 kb on - strandat 4319.731 kb on - strandat 4319.803 kb on + strand, within phnFat 4319.803 kb on + strand, within phnFat 4319.803 kb on + strand, within phnFat 4319.811 kb on - strand, within phnFat 4319.811 kb on - strand, within phnFat 4319.985 kb on - strand, within phnFat 4319.999 kb on + strand, within phnFat 4320.073 kb on - strand, within phnFat 4320.121 kb on - strand, within phnFat 4320.253 kb on - strand, within phnFat 4320.253 kb on - strand, within phnFat 4320.292 kb on + strand, within phnFat 4320.326 kb on + strand, within phnFat 4320.348 kb on - strand, within phnFat 4320.405 kb on - strandat 4320.412 kb on + strandat 4320.412 kb on + strandat 4320.652 kb on - strand, within phnEat 4320.670 kb on - strand, within phnEat 4320.678 kb on - strand, within phnEat 4320.691 kb on + strand, within phnEat 4320.804 kb on + strandat 4320.842 kb on + strandat 4320.937 kb on + strandat 4321.008 kb on - strandat 4321.144 kb on - strandat 4321.193 kb on - strandat 4321.193 kb on - strandat 4321.193 kb on - strandat 4321.208 kb on - strandat 4321.248 kb on - strandat 4321.254 kb on + strandat 4321.308 kb on + strandat 4321.310 kb on + strandat 4321.310 kb on + strandat 4321.316 kb on - strandat 4321.421 kb on - strandat 4321.440 kb on - strandat 4321.440 kb on - strandat 4321.512 kb on - strand, within phnDat 4321.572 kb on + strand, within phnDat 4321.637 kb on - strand, within phnDat 4321.637 kb on - strand, within phnDat 4321.713 kb on - strand, within phnDat 4321.713 kb on - strand, within phnDat 4321.716 kb on + strand, within phnDat 4321.834 kb on + strand, within phnDat 4321.834 kb on + strand, within phnD

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Per-strain Table

Position Strand Gene LocusTag Fraction Phosphomycin 0.001 mg/ml
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4,319,573 + phnG b4101 0.68 -0.2
4,319,731 - +12.8
4,319,731 - +11.4
4,319,803 + phnF b4102 0.11 -0.4
4,319,803 + phnF b4102 0.11 +1.7
4,319,803 + phnF b4102 0.11 -0.2
4,319,811 - phnF b4102 0.13 +12.3
4,319,811 - phnF b4102 0.13 +11.0
4,319,985 - phnF b4102 0.37 +11.7
4,319,999 + phnF b4102 0.38 +1.4
4,320,073 - phnF b4102 0.49 +12.1
4,320,121 - phnF b4102 0.55 +12.1
4,320,253 - phnF b4102 0.73 +12.4
4,320,253 - phnF b4102 0.73 +12.2
4,320,292 + phnF b4102 0.79 -0.4
4,320,326 + phnF b4102 0.83 -0.0
4,320,348 - phnF b4102 0.87 +11.3
4,320,405 - +1.9
4,320,412 + +0.3
4,320,412 + -0.2
4,320,652 - phnE b4104 0.50 +11.8
4,320,670 - phnE b4104 0.55 +12.0
4,320,678 - phnE b4104 0.57 +12.3
4,320,691 + phnE b4104 0.60 -0.1
4,320,804 + -1.0
4,320,842 + -0.5
4,320,937 + -0.1
4,321,008 - +8.6
4,321,144 - +9.2
4,321,193 - +9.1
4,321,193 - +0.6
4,321,193 - +1.3
4,321,208 - +4.5
4,321,248 - +11.3
4,321,254 + +0.2
4,321,308 + -0.8
4,321,310 + -1.2
4,321,310 + +0.1
4,321,316 - +11.4
4,321,421 - +12.1
4,321,440 - +12.2
4,321,440 - +12.5
4,321,512 - phnD b4105 0.15 +12.2
4,321,572 + phnD b4105 0.21 -0.6
4,321,637 - phnD b4105 0.27 +11.9
4,321,637 - phnD b4105 0.27 +11.9
4,321,713 - phnD b4105 0.35 +11.2
4,321,713 - phnD b4105 0.35 +11.7
4,321,716 + phnD b4105 0.35 -0.3
4,321,834 + phnD b4105 0.47 -0.1
4,321,834 + phnD b4105 0.47 +0.6

Or see this region's nucleotide sequence