Experiment: Phosphomycin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnI and phnH overlap by 1 nucleotides phnH and phnG overlap by 4 nucleotides phnG and phnF are separated by 0 nucleotides phnF and phnE are separated by 20 nucleotides
b4099: phnI - carbon-phosphorus lyase complex subunit (NCBI), at 4,317,622 to 4,318,686
phnI
b4100: phnH - carbon-phosphorus lyase complex subunit (NCBI), at 4,318,686 to 4,319,270
phnH
b4101: phnG - carbon-phosphorus lyase complex subunit (NCBI), at 4,319,267 to 4,319,719
phnG
b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445
phnF
b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837
phnE
Position (kb) 4319
4320 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10
11
12
13 at 4318.321 kb on - strand, within phnI at 4318.326 kb on - strand, within phnI at 4318.392 kb on + strand, within phnI at 4318.400 kb on + strand, within phnI at 4318.489 kb on - strand, within phnI at 4318.549 kb on + strand, within phnI at 4318.635 kb on - strand at 4318.635 kb on - strand at 4318.687 kb on - strand at 4318.702 kb on - strand at 4318.748 kb on - strand, within phnH at 4318.799 kb on - strand, within phnH at 4318.814 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4318.855 kb on + strand, within phnH at 4319.165 kb on + strand, within phnH at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.223 kb on + strand at 4319.228 kb on + strand at 4319.258 kb on + strand at 4319.258 kb on + strand at 4319.259 kb on + strand at 4319.274 kb on - strand at 4319.279 kb on - strand at 4319.279 kb on - strand at 4319.354 kb on - strand, within phnG at 4319.366 kb on - strand, within phnG at 4319.379 kb on - strand, within phnG at 4319.434 kb on - strand, within phnG at 4319.446 kb on - strand, within phnG at 4319.573 kb on + strand, within phnG at 4319.731 kb on - strand at 4319.731 kb on - strand at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.803 kb on + strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.811 kb on - strand, within phnF at 4319.985 kb on - strand, within phnF at 4319.999 kb on + strand, within phnF at 4320.073 kb on - strand, within phnF at 4320.121 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF at 4320.253 kb on - strand, within phnF at 4320.292 kb on + strand, within phnF at 4320.326 kb on + strand, within phnF at 4320.348 kb on - strand, within phnF at 4320.405 kb on - strand at 4320.412 kb on + strand at 4320.412 kb on + strand at 4320.652 kb on - strand, within phnE at 4320.670 kb on - strand, within phnE at 4320.678 kb on - strand, within phnE at 4320.691 kb on + strand, within phnE
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.001 mg/ml remove 4,318,321 - phnI b4099 0.66 +11.0 4,318,326 - phnI b4099 0.66 -0.0 4,318,392 + phnI b4099 0.72 -0.1 4,318,400 + phnI b4099 0.73 +0.1 4,318,489 - phnI b4099 0.81 +9.4 4,318,549 + phnI b4099 0.87 -0.4 4,318,635 - +0.1 4,318,635 - +0.2 4,318,687 - +8.1 4,318,702 - +9.6 4,318,748 - phnH b4100 0.11 +6.6 4,318,799 - phnH b4100 0.19 +4.8 4,318,814 + phnH b4100 0.22 -0.3 4,318,855 + phnH b4100 0.29 -1.8 4,318,855 + phnH b4100 0.29 -0.6 4,319,165 + phnH b4100 0.82 +0.1 4,319,223 + -0.1 4,319,223 + -0.3 4,319,223 + -0.9 4,319,228 + -0.4 4,319,258 + -0.1 4,319,258 + -0.7 4,319,259 + -0.6 4,319,274 - +6.4 4,319,279 - +5.1 4,319,279 - +9.2 4,319,354 - phnG b4101 0.19 +9.6 4,319,366 - phnG b4101 0.22 +10.3 4,319,379 - phnG b4101 0.25 +10.3 4,319,434 - phnG b4101 0.37 +11.3 4,319,446 - phnG b4101 0.40 +10.2 4,319,573 + phnG b4101 0.68 -0.2 4,319,731 - +12.8 4,319,731 - +11.4 4,319,803 + phnF b4102 0.11 -0.4 4,319,803 + phnF b4102 0.11 +1.7 4,319,803 + phnF b4102 0.11 -0.2 4,319,811 - phnF b4102 0.13 +12.3 4,319,811 - phnF b4102 0.13 +11.0 4,319,985 - phnF b4102 0.37 +11.7 4,319,999 + phnF b4102 0.38 +1.4 4,320,073 - phnF b4102 0.49 +12.1 4,320,121 - phnF b4102 0.55 +12.1 4,320,253 - phnF b4102 0.73 +12.4 4,320,253 - phnF b4102 0.73 +12.2 4,320,292 + phnF b4102 0.79 -0.4 4,320,326 + phnF b4102 0.83 -0.0 4,320,348 - phnF b4102 0.87 +11.3 4,320,405 - +1.9 4,320,412 + +0.3 4,320,412 + -0.2 4,320,652 - phnE b4104 0.50 +11.8 4,320,670 - phnE b4104 0.55 +12.0 4,320,678 - phnE b4104 0.57 +12.3 4,320,691 + phnE b4104 0.60 -0.1
Or see this region's nucleotide sequence