Experiment: Nalidixic 0.006 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaY and yeaZ are separated by 39 nucleotides yeaZ and yoaA are separated by 57 nucleotides yoaA and b1809 are separated by 131 nucleotides b1809 and yoaC are separated by 421 nucleotides
b1806: yeaY - predicted lipoprotein (NCBI), at 1,887,975 to 1,888,556
yeaY
b1807: yeaZ - predicted peptidase (NCBI), at 1,888,596 to 1,889,291
yeaZ
b1808: yoaA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,889,349 to 1,891,259
yoaA
b1809: b1809 - orf, hypothetical protein (VIMSS), at 1,891,391 to 1,891,735
b1809
b1810: yoaC - predicted protein (RefSeq), at 1,892,157 to 1,892,456
yoaC
Position (kb)
1889
1890
1891
1892 Strain fitness (log2 ratio)
-2
-1
0
1 at 1888.428 kb on - strand, within yeaY at 1888.428 kb on - strand, within yeaY at 1888.463 kb on + strand, within yeaY at 1888.586 kb on + strand at 1888.586 kb on + strand at 1889.333 kb on - strand at 1889.364 kb on - strand at 1889.751 kb on + strand, within yoaA at 1889.811 kb on - strand, within yoaA at 1889.917 kb on + strand, within yoaA at 1889.917 kb on + strand, within yoaA at 1890.135 kb on - strand, within yoaA at 1890.148 kb on - strand, within yoaA at 1890.187 kb on + strand, within yoaA at 1890.311 kb on + strand, within yoaA at 1890.503 kb on - strand, within yoaA at 1890.762 kb on - strand, within yoaA at 1890.858 kb on - strand, within yoaA at 1890.993 kb on + strand, within yoaA at 1891.001 kb on - strand, within yoaA at 1891.001 kb on - strand, within yoaA at 1891.023 kb on - strand, within yoaA at 1891.209 kb on + strand at 1891.209 kb on + strand at 1891.260 kb on + strand at 1891.403 kb on - strand at 1891.519 kb on + strand, within b1809 at 1891.533 kb on - strand, within b1809 at 1891.533 kb on - strand, within b1809 at 1891.630 kb on + strand, within b1809 at 1891.632 kb on + strand, within b1809 at 1891.632 kb on + strand, within b1809 at 1891.658 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.673 kb on + strand, within b1809 at 1891.679 kb on - strand, within b1809 at 1891.681 kb on - strand, within b1809 at 1891.730 kb on + strand at 1891.730 kb on + strand at 1891.730 kb on + strand at 1891.737 kb on - strand at 1891.738 kb on - strand at 1891.750 kb on + strand at 1891.887 kb on + strand at 1891.887 kb on + strand at 1891.892 kb on + strand at 1891.892 kb on + strand at 1891.913 kb on - strand at 1891.932 kb on - strand at 1891.951 kb on + strand at 1891.959 kb on - strand at 1891.965 kb on + strand at 1891.981 kb on - strand at 1892.003 kb on + strand at 1892.008 kb on + strand at 1892.010 kb on + strand at 1892.016 kb on - strand at 1892.016 kb on - strand at 1892.032 kb on + strand at 1892.032 kb on + strand at 1892.046 kb on + strand at 1892.046 kb on + strand at 1892.102 kb on + strand at 1892.102 kb on + strand at 1892.153 kb on + strand at 1892.166 kb on + strand at 1892.178 kb on + strand at 1892.178 kb on + strand at 1892.196 kb on + strand, within yoaC at 1892.196 kb on + strand, within yoaC at 1892.199 kb on + strand, within yoaC at 1892.201 kb on + strand, within yoaC at 1892.226 kb on - strand, within yoaC at 1892.226 kb on - strand, within yoaC at 1892.230 kb on - strand, within yoaC
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.006 mg/ml remove 1,888,428 - yeaY b1806 0.78 -0.5 1,888,428 - yeaY b1806 0.78 -0.0 1,888,463 + yeaY b1806 0.84 -0.1 1,888,586 + +0.8 1,888,586 + +0.3 1,889,333 - +0.5 1,889,364 - +1.2 1,889,751 + yoaA b1808 0.21 +0.9 1,889,811 - yoaA b1808 0.24 +0.8 1,889,917 + yoaA b1808 0.30 +1.3 1,889,917 + yoaA b1808 0.30 +1.1 1,890,135 - yoaA b1808 0.41 +1.4 1,890,148 - yoaA b1808 0.42 +1.3 1,890,187 + yoaA b1808 0.44 +0.9 1,890,311 + yoaA b1808 0.50 +0.9 1,890,503 - yoaA b1808 0.60 +1.6 1,890,762 - yoaA b1808 0.74 +1.2 1,890,858 - yoaA b1808 0.79 +1.4 1,890,993 + yoaA b1808 0.86 +1.2 1,891,001 - yoaA b1808 0.86 +1.6 1,891,001 - yoaA b1808 0.86 +1.1 1,891,023 - yoaA b1808 0.88 +1.1 1,891,209 + +0.9 1,891,209 + +1.4 1,891,260 + +0.4 1,891,403 - -0.1 1,891,519 + b1809 b1809 0.37 -0.6 1,891,533 - b1809 b1809 0.41 -0.3 1,891,533 - b1809 b1809 0.41 +0.1 1,891,630 + b1809 b1809 0.69 +1.0 1,891,632 + b1809 b1809 0.70 -1.1 1,891,632 + b1809 b1809 0.70 -0.3 1,891,658 + b1809 b1809 0.77 -0.5 1,891,673 + b1809 b1809 0.82 -0.6 1,891,673 + b1809 b1809 0.82 -0.6 1,891,673 + b1809 b1809 0.82 +0.1 1,891,673 + b1809 b1809 0.82 -0.7 1,891,673 + b1809 b1809 0.82 -0.3 1,891,673 + b1809 b1809 0.82 -0.4 1,891,673 + b1809 b1809 0.82 +0.5 1,891,679 - b1809 b1809 0.83 -0.1 1,891,681 - b1809 b1809 0.84 -0.0 1,891,730 + +0.2 1,891,730 + -1.1 1,891,730 + -0.2 1,891,737 - -2.0 1,891,738 - -1.0 1,891,750 + +1.0 1,891,887 + -0.8 1,891,887 + -0.0 1,891,892 + -1.8 1,891,892 + +0.8 1,891,913 - -0.6 1,891,932 - -0.3 1,891,951 + -1.0 1,891,959 - -0.4 1,891,965 + +0.5 1,891,981 - -0.7 1,892,003 + -0.1 1,892,008 + -0.1 1,892,010 + +0.1 1,892,016 - +0.3 1,892,016 - -0.6 1,892,032 + -0.6 1,892,032 + +0.4 1,892,046 + -0.5 1,892,046 + -0.3 1,892,102 + -0.0 1,892,102 + -0.4 1,892,153 + -0.6 1,892,166 + -0.4 1,892,178 + -0.6 1,892,178 + -0.1 1,892,196 + yoaC b1810 0.13 -0.4 1,892,196 + yoaC b1810 0.13 -0.2 1,892,199 + yoaC b1810 0.14 -0.4 1,892,201 + yoaC b1810 0.15 +0.5 1,892,226 - yoaC b1810 0.23 -0.8 1,892,226 - yoaC b1810 0.23 +1.7 1,892,230 - yoaC b1810 0.24 -1.2
Or see this region's nucleotide sequence