Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3878 and GFF3879 are separated by 2 nucleotides GFF3879 and GFF3880 are separated by 110 nucleotides
GFF3878 - Colanic acid biosysnthesis glycosyl transferase WcaI, at 153,690 to 154,913
GFF3878
GFF3879 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 154,916 to 156,352
GFF3879
GFF3880 - Phosphomannomutase (EC 5.4.2.8), at 156,463 to 157,833
GFF3880
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -2
-1
0
1 at 154.091 kb on - strand, within GFF3878 at 154.456 kb on + strand, within GFF3878 at 154.700 kb on + strand, within GFF3878 at 154.882 kb on + strand at 154.914 kb on + strand at 154.994 kb on + strand at 155.143 kb on + strand, within GFF3879 at 155.393 kb on + strand, within GFF3879 at 155.552 kb on + strand, within GFF3879 at 155.855 kb on + strand, within GFF3879 at 156.023 kb on + strand, within GFF3879 at 156.082 kb on + strand, within GFF3879 at 156.191 kb on - strand, within GFF3879 at 156.218 kb on + strand at 156.276 kb on + strand at 156.276 kb on + strand at 156.435 kb on + strand at 156.490 kb on + strand at 156.681 kb on + strand, within GFF3880 at 156.682 kb on - strand, within GFF3880 at 156.772 kb on + strand, within GFF3880 at 156.773 kb on - strand, within GFF3880 at 156.898 kb on + strand, within GFF3880 at 156.899 kb on - strand, within GFF3880 at 156.925 kb on + strand, within GFF3880 at 157.255 kb on + strand, within GFF3880 at 157.256 kb on - strand, within GFF3880
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 154,091 - GFF3878 0.33 +1.3 154,456 + GFF3878 0.63 -0.5 154,700 + GFF3878 0.83 -0.4 154,882 + +0.1 154,914 + -0.3 154,994 + -0.5 155,143 + GFF3879 0.16 +0.1 155,393 + GFF3879 0.33 +0.3 155,552 + GFF3879 0.44 +0.6 155,855 + GFF3879 0.65 +0.8 156,023 + GFF3879 0.77 +0.4 156,082 + GFF3879 0.81 -0.1 156,191 - GFF3879 0.89 -0.0 156,218 + -0.4 156,276 + -0.8 156,276 + +0.1 156,435 + +0.1 156,490 + -1.1 156,681 + GFF3880 0.16 -1.5 156,682 - GFF3880 0.16 +0.3 156,772 + GFF3880 0.23 -1.8 156,773 - GFF3880 0.23 -0.4 156,898 + GFF3880 0.32 -0.9 156,899 - GFF3880 0.32 +0.3 156,925 + GFF3880 0.34 +0.8 157,255 + GFF3880 0.58 +0.0 157,256 - GFF3880 0.58 -0.2
Or see this region's nucleotide sequence