Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2122 and GFF2123 are separated by 85 nucleotides GFF2123 and GFF2124 are separated by 25 nucleotides
GFF2122 - Adenine-specific methyltransferase (EC 2.1.1.72), at 9,391 to 10,275
GFF2122
GFF2123 - DNA-binding protein Fis, at 10,361 to 10,657
GFF2123
GFF2124 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 10,683 to 11,528
GFF2124
Position (kb)
10
11 Strain fitness (log2 ratio)
-1
0
1 at 9.653 kb on - strand, within GFF2122 at 9.702 kb on - strand, within GFF2122 at 9.716 kb on + strand, within GFF2122 at 9.717 kb on - strand, within GFF2122 at 9.767 kb on + strand, within GFF2122 at 9.897 kb on - strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.900 kb on + strand, within GFF2122 at 9.936 kb on + strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.937 kb on - strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.941 kb on + strand, within GFF2122 at 9.999 kb on - strand, within GFF2122 at 10.028 kb on + strand, within GFF2122 at 10.029 kb on - strand, within GFF2122 at 10.080 kb on - strand, within GFF2122 at 10.507 kb on - strand, within GFF2123 at 10.629 kb on - strand at 10.809 kb on - strand, within GFF2124 at 11.004 kb on - strand, within GFF2124 at 11.037 kb on - strand, within GFF2124 at 11.081 kb on + strand, within GFF2124 at 11.194 kb on - strand, within GFF2124 at 11.202 kb on - strand, within GFF2124 at 11.280 kb on - strand, within GFF2124 at 11.290 kb on - strand, within GFF2124 at 11.327 kb on + strand, within GFF2124 at 11.369 kb on + strand, within GFF2124 at 11.396 kb on + strand, within GFF2124 at 11.397 kb on - strand, within GFF2124 at 11.493 kb on - strand at 11.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 9,653 - GFF2122 0.30 +0.4 9,702 - GFF2122 0.35 -0.6 9,716 + GFF2122 0.37 +0.3 9,717 - GFF2122 0.37 -0.7 9,767 + GFF2122 0.42 -0.1 9,897 - GFF2122 0.57 -0.4 9,900 + GFF2122 0.58 +0.5 9,900 + GFF2122 0.58 +0.6 9,936 + GFF2122 0.62 +0.4 9,937 - GFF2122 0.62 -0.3 9,937 - GFF2122 0.62 -0.1 9,941 + GFF2122 0.62 -0.8 9,941 + GFF2122 0.62 -0.0 9,999 - GFF2122 0.69 +0.3 10,028 + GFF2122 0.72 -0.3 10,029 - GFF2122 0.72 +0.2 10,080 - GFF2122 0.78 -0.2 10,507 - GFF2123 0.49 -0.2 10,629 - -0.4 10,809 - GFF2124 0.15 +1.3 11,004 - GFF2124 0.38 +0.6 11,037 - GFF2124 0.42 +0.9 11,081 + GFF2124 0.47 +1.2 11,194 - GFF2124 0.60 -0.3 11,202 - GFF2124 0.61 +0.5 11,280 - GFF2124 0.71 -0.0 11,290 - GFF2124 0.72 +0.4 11,327 + GFF2124 0.76 +0.5 11,369 + GFF2124 0.81 +0.5 11,396 + GFF2124 0.84 +1.5 11,397 - GFF2124 0.84 -0.4 11,493 - -0.1 11,561 - +0.2
Or see this region's nucleotide sequence