Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF89 and GFF90 overlap by 4 nucleotides GFF90 and GFF91 are separated by 120 nucleotides GFF91 and GFF92 are separated by 125 nucleotides
GFF89 - Poly(A) polymerase (EC 2.7.7.19), at 73,852 to 75,216
GFF89
GFF90 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3), at 75,213 to 75,692
GFF90
GFF91 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 75,813 to 76,604
GFF91
GFF92 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 76,730 to 77,584
GFF92
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 74.842 kb on + strand, within GFF89 at 74.916 kb on + strand, within GFF89 at 74.934 kb on + strand, within GFF89 at 75.636 kb on - strand, within GFF90 at 75.773 kb on - strand at 75.773 kb on - strand at 75.796 kb on + strand at 75.907 kb on + strand, within GFF91 at 75.923 kb on + strand, within GFF91 at 75.924 kb on - strand, within GFF91 at 76.005 kb on - strand, within GFF91 at 76.051 kb on + strand, within GFF91 at 76.074 kb on - strand, within GFF91 at 76.248 kb on - strand, within GFF91 at 76.318 kb on + strand, within GFF91 at 76.484 kb on + strand, within GFF91 at 76.548 kb on + strand at 76.548 kb on + strand at 76.548 kb on + strand at 76.549 kb on - strand at 76.549 kb on - strand at 76.550 kb on + strand at 76.551 kb on - strand at 76.551 kb on - strand at 76.551 kb on - strand at 76.679 kb on + strand at 76.734 kb on + strand at 76.769 kb on - strand at 76.994 kb on + strand, within GFF92 at 77.051 kb on + strand, within GFF92 at 77.051 kb on + strand, within GFF92 at 77.052 kb on - strand, within GFF92 at 77.297 kb on - strand, within GFF92 at 77.304 kb on - strand, within GFF92 at 77.342 kb on + strand, within GFF92 at 77.356 kb on + strand, within GFF92 at 77.357 kb on - strand, within GFF92 at 77.405 kb on - strand, within GFF92
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 74,842 + GFF89 0.73 -0.2 74,916 + GFF89 0.78 -0.3 74,934 + GFF89 0.79 +0.4 75,636 - GFF90 0.88 +2.0 75,773 - -0.1 75,773 - -0.6 75,796 + -0.2 75,907 + GFF91 0.12 -0.8 75,923 + GFF91 0.14 -0.6 75,924 - GFF91 0.14 -0.5 76,005 - GFF91 0.24 -0.1 76,051 + GFF91 0.30 +0.1 76,074 - GFF91 0.33 +0.1 76,248 - GFF91 0.55 +0.1 76,318 + GFF91 0.64 -0.8 76,484 + GFF91 0.85 -0.5 76,548 + +1.1 76,548 + +0.3 76,548 + -0.2 76,549 - -0.1 76,549 - -2.1 76,550 + -0.6 76,551 - -0.2 76,551 - -0.3 76,551 - -0.5 76,679 + -0.2 76,734 + +0.4 76,769 - -0.2 76,994 + GFF92 0.31 +0.3 77,051 + GFF92 0.38 +0.2 77,051 + GFF92 0.38 -3.5 77,052 - GFF92 0.38 +0.1 77,297 - GFF92 0.66 -0.5 77,304 - GFF92 0.67 -0.2 77,342 + GFF92 0.72 -0.7 77,356 + GFF92 0.73 +0.5 77,357 - GFF92 0.73 +0.2 77,405 - GFF92 0.79 +1.2
Or see this region's nucleotide sequence