Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF427 and GFF428 overlap by 8 nucleotides GFF428 and GFF429 overlap by 4 nucleotides GFF429 and GFF430 are separated by 3 nucleotides
GFF427 - L-xylulose 5-phosphate 3-epimerase (EC 5.1.3.-) homolog, at 149,107 to 149,967
GFF427
GFF428 - 3-keto-L-gulonate 6-phosphate decarboxylase homolog, at 149,960 to 150,622
GFF428
GFF429 - L-xylulose/3-keto-L-gulonate kinase (EC 2.7.1.-), at 150,619 to 152,115
GFF429
GFF430 - 2,3-diketo-L-gulonate-binding periplasmic protein yiaO precursor, at 152,119 to 153,105
GFF430
Position (kb)
150
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 149.950 kb on + strand at 149.951 kb on - strand at 150.219 kb on + strand, within GFF428 at 150.367 kb on + strand, within GFF428 at 150.368 kb on - strand, within GFF428 at 150.609 kb on - strand at 150.658 kb on + strand at 150.659 kb on - strand at 150.659 kb on - strand at 150.785 kb on - strand, within GFF429 at 150.999 kb on - strand, within GFF429 at 150.999 kb on - strand, within GFF429 at 151.230 kb on + strand, within GFF429 at 151.230 kb on + strand, within GFF429 at 151.232 kb on + strand, within GFF429 at 151.233 kb on - strand, within GFF429 at 151.260 kb on - strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.265 kb on + strand, within GFF429 at 151.544 kb on + strand, within GFF429 at 151.566 kb on - strand, within GFF429 at 151.631 kb on + strand, within GFF429 at 151.658 kb on - strand, within GFF429 at 151.667 kb on + strand, within GFF429 at 151.700 kb on - strand, within GFF429 at 151.700 kb on - strand, within GFF429 at 151.832 kb on - strand, within GFF429 at 152.005 kb on + strand at 152.036 kb on - strand at 152.058 kb on - strand at 152.071 kb on + strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.121 kb on - strand at 152.182 kb on - strand at 152.309 kb on + strand, within GFF430 at 152.382 kb on + strand, within GFF430 at 152.433 kb on - strand, within GFF430 at 152.433 kb on - strand, within GFF430 at 152.441 kb on - strand, within GFF430 at 152.475 kb on + strand, within GFF430 at 152.475 kb on + strand, within GFF430 at 152.476 kb on - strand, within GFF430 at 152.565 kb on + strand, within GFF430 at 152.797 kb on + strand, within GFF430 at 152.797 kb on + strand, within GFF430 at 152.798 kb on - strand, within GFF430 at 152.798 kb on - strand, within GFF430
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 149,950 + -0.2 149,951 - -0.5 150,219 + GFF428 0.39 -1.3 150,367 + GFF428 0.61 +0.2 150,368 - GFF428 0.62 -0.3 150,609 - -2.0 150,658 + -0.6 150,659 - -0.2 150,659 - +0.0 150,785 - GFF429 0.11 -0.9 150,999 - GFF429 0.25 +0.2 150,999 - GFF429 0.25 -0.5 151,230 + GFF429 0.41 -0.8 151,230 + GFF429 0.41 +0.2 151,232 + GFF429 0.41 -1.0 151,233 - GFF429 0.41 +0.1 151,260 - GFF429 0.43 -0.1 151,265 + GFF429 0.43 -0.0 151,265 + GFF429 0.43 -0.0 151,265 + GFF429 0.43 -0.5 151,544 + GFF429 0.62 +1.0 151,566 - GFF429 0.63 -0.1 151,631 + GFF429 0.68 +0.2 151,658 - GFF429 0.69 +0.2 151,667 + GFF429 0.70 +0.1 151,700 - GFF429 0.72 +0.2 151,700 - GFF429 0.72 -0.1 151,832 - GFF429 0.81 -0.3 152,005 + -0.3 152,036 - +0.1 152,058 - +0.1 152,071 + -0.1 152,121 - -0.3 152,121 - -0.2 152,121 - +0.1 152,121 - -0.4 152,121 - -0.2 152,182 - -0.1 152,309 + GFF430 0.19 +0.7 152,382 + GFF430 0.27 +0.1 152,433 - GFF430 0.32 -0.0 152,433 - GFF430 0.32 -0.1 152,441 - GFF430 0.33 +0.5 152,475 + GFF430 0.36 +0.2 152,475 + GFF430 0.36 -0.2 152,476 - GFF430 0.36 -0.4 152,565 + GFF430 0.45 +0.1 152,797 + GFF430 0.69 -0.3 152,797 + GFF430 0.69 -0.2 152,798 - GFF430 0.69 -0.3 152,798 - GFF430 0.69 -0.5
Or see this region's nucleotide sequence