Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3584 and GFF3585 overlap by 8 nucleotides GFF3585 and GFF3586 are separated by 16 nucleotides GFF3586 and GFF3587 are separated by 263 nucleotides GFF3587 and GFF3588 are separated by 6 nucleotides
GFF3584 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 342,455 to 343,948
GFF3584
GFF3585 - Secretion system apparatus SsaD, at 343,941 to 345,140
GFF3585
GFF3586 - Secretion system effector SsaE, at 345,157 to 345,390
GFF3586
GFF3587 - Secretion system effector SseA, at 345,654 to 345,980
GFF3587
GFF3588 - Secretion system effector SseB, at 345,987 to 346,577
GFF3588
Position (kb) 343
344
345
346 Strain fitness (log2 ratio) -2
-1
0
1 at 342.985 kb on - strand, within GFF3584 at 342.989 kb on - strand, within GFF3584 at 342.990 kb on + strand, within GFF3584 at 342.991 kb on - strand, within GFF3584 at 343.000 kb on + strand, within GFF3584 at 343.000 kb on + strand, within GFF3584 at 343.001 kb on - strand, within GFF3584 at 343.367 kb on - strand, within GFF3584 at 344.257 kb on + strand, within GFF3585 at 344.686 kb on + strand, within GFF3585 at 344.837 kb on - strand, within GFF3585 at 345.134 kb on + strand at 345.221 kb on + strand, within GFF3586 at 345.221 kb on + strand, within GFF3586 at 345.426 kb on + strand at 345.426 kb on + strand at 345.476 kb on - strand at 345.643 kb on + strand at 346.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 342,985 - GFF3584 0.35 -1.6 342,989 - GFF3584 0.36 -2.1 342,990 + GFF3584 0.36 -0.1 342,991 - GFF3584 0.36 +1.2 343,000 + GFF3584 0.36 -0.0 343,000 + GFF3584 0.36 -0.0 343,001 - GFF3584 0.37 -0.1 343,367 - GFF3584 0.61 -0.8 344,257 + GFF3585 0.26 -1.0 344,686 + GFF3585 0.62 -0.1 344,837 - GFF3585 0.75 -0.6 345,134 + -1.1 345,221 + GFF3586 0.27 -0.5 345,221 + GFF3586 0.27 -1.7 345,426 + -1.0 345,426 + +0.4 345,476 - -0.9 345,643 + +0.4 346,006 + +0.4
Or see this region's nucleotide sequence