Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3206 and GFF3207 are separated by 69 nucleotides GFF3207 and GFF3208 are separated by 33 nucleotides
GFF3206 - L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17), at 210,133 to 211,497
GFF3206
GFF3207 - 2-ketobutyrate formate-lyase (EC 2.3.1.-) / Pyruvate formate-lyase (EC 2.3.1.54), at 211,567 to 213,861
GFF3207
GFF3208 - Acetate kinase (EC 2.7.2.1), at 213,895 to 215,103
GFF3208
Position (kb) 211
212
213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 210.600 kb on - strand, within GFF3206 at 210.705 kb on + strand, within GFF3206 at 211.068 kb on - strand, within GFF3206 at 211.070 kb on + strand, within GFF3206 at 211.113 kb on + strand, within GFF3206 at 211.114 kb on - strand, within GFF3206 at 211.161 kb on + strand, within GFF3206 at 211.278 kb on + strand, within GFF3206 at 211.497 kb on + strand at 211.497 kb on + strand at 211.497 kb on + strand at 211.497 kb on + strand at 211.568 kb on + strand at 211.569 kb on - strand at 211.582 kb on - strand at 211.597 kb on - strand at 211.750 kb on + strand at 211.751 kb on - strand at 211.751 kb on - strand at 211.751 kb on - strand at 212.066 kb on + strand, within GFF3207 at 212.150 kb on + strand, within GFF3207 at 212.336 kb on + strand, within GFF3207 at 212.351 kb on + strand, within GFF3207 at 212.352 kb on - strand, within GFF3207 at 212.378 kb on + strand, within GFF3207 at 212.378 kb on + strand, within GFF3207 at 212.432 kb on + strand, within GFF3207 at 212.432 kb on + strand, within GFF3207 at 212.433 kb on - strand, within GFF3207 at 212.433 kb on - strand, within GFF3207 at 212.516 kb on + strand, within GFF3207 at 212.691 kb on - strand, within GFF3207 at 212.691 kb on - strand, within GFF3207 at 212.729 kb on - strand, within GFF3207 at 212.729 kb on - strand, within GFF3207 at 212.772 kb on + strand, within GFF3207 at 212.773 kb on - strand, within GFF3207 at 212.990 kb on + strand, within GFF3207 at 212.991 kb on - strand, within GFF3207 at 212.994 kb on + strand, within GFF3207 at 212.994 kb on + strand, within GFF3207 at 212.995 kb on - strand, within GFF3207 at 213.071 kb on + strand, within GFF3207 at 213.333 kb on - strand, within GFF3207 at 213.333 kb on - strand, within GFF3207 at 213.403 kb on + strand, within GFF3207 at 213.414 kb on + strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.442 kb on - strand, within GFF3207 at 213.473 kb on + strand, within GFF3207 at 213.665 kb on - strand at 213.749 kb on + strand at 213.753 kb on + strand at 213.754 kb on - strand at 213.828 kb on - strand at 214.072 kb on + strand, within GFF3208 at 214.159 kb on + strand, within GFF3208 at 214.160 kb on - strand, within GFF3208 at 214.443 kb on + strand, within GFF3208 at 214.637 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 210,600 - GFF3206 0.34 -0.1 210,705 + GFF3206 0.42 +1.1 211,068 - GFF3206 0.68 +0.3 211,070 + GFF3206 0.69 -0.1 211,113 + GFF3206 0.72 +0.7 211,114 - GFF3206 0.72 -0.1 211,161 + GFF3206 0.75 +0.4 211,278 + GFF3206 0.84 +1.5 211,497 + +0.5 211,497 + +0.0 211,497 + -0.7 211,497 + +0.1 211,568 + +0.3 211,569 - +1.0 211,582 - +0.1 211,597 - +0.1 211,750 + +0.1 211,751 - +0.2 211,751 - +0.3 211,751 - -1.3 212,066 + GFF3207 0.22 +0.1 212,150 + GFF3207 0.25 -0.1 212,336 + GFF3207 0.34 +0.0 212,351 + GFF3207 0.34 +0.1 212,352 - GFF3207 0.34 -0.1 212,378 + GFF3207 0.35 +0.1 212,378 + GFF3207 0.35 +0.5 212,432 + GFF3207 0.38 -0.1 212,432 + GFF3207 0.38 +1.8 212,433 - GFF3207 0.38 +0.1 212,433 - GFF3207 0.38 +0.3 212,516 + GFF3207 0.41 -0.2 212,691 - GFF3207 0.49 -1.7 212,691 - GFF3207 0.49 +1.0 212,729 - GFF3207 0.51 +0.2 212,729 - GFF3207 0.51 +0.1 212,772 + GFF3207 0.53 +0.2 212,773 - GFF3207 0.53 +0.1 212,990 + GFF3207 0.62 -1.2 212,991 - GFF3207 0.62 +0.6 212,994 + GFF3207 0.62 +0.9 212,994 + GFF3207 0.62 +0.1 212,995 - GFF3207 0.62 +0.2 213,071 + GFF3207 0.66 +0.8 213,333 - GFF3207 0.77 -1.1 213,333 - GFF3207 0.77 +0.2 213,403 + GFF3207 0.80 -0.8 213,414 + GFF3207 0.80 -0.4 213,442 - GFF3207 0.82 -0.9 213,442 - GFF3207 0.82 +0.6 213,442 - GFF3207 0.82 +0.1 213,442 - GFF3207 0.82 +0.2 213,473 + GFF3207 0.83 +0.2 213,665 - -0.6 213,749 + -0.1 213,753 + -0.4 213,754 - +0.4 213,828 - -3.7 214,072 + GFF3208 0.15 +0.5 214,159 + GFF3208 0.22 +0.9 214,160 - GFF3208 0.22 +0.5 214,443 + GFF3208 0.45 -0.0 214,637 - GFF3208 0.61 +0.7 214,807 - GFF3208 0.75 -0.2 214,807 - GFF3208 0.75 +0.8 214,826 - GFF3208 0.77 -0.7 214,826 - GFF3208 0.77 -0.1
Or see this region's nucleotide sequence