Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3108 and GFF3109 are separated by 7 nucleotides GFF3109 and GFF3110 overlap by 4 nucleotides GFF3110 and GFF3111 are separated by 84 nucleotides GFF3111 and GFF3112 are separated by 306 nucleotides
GFF3108 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 117,779 to 118,897
GFF3108
GFF3109 - FIG01046062: hypothetical protein, at 118,905 to 119,090
GFF3109
GFF3110 - FIG00638753: hypothetical protein, at 119,087 to 119,374
GFF3110
GFF3111 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 119,459 to 121,102
GFF3111
GFF3112 - Putative membrane protein, at 121,409 to 121,903
GFF3112
Position (kb)
119
120
121
122 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 118.512 kb on + strand, within GFF3108 at 118.513 kb on - strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.641 kb on + strand, within GFF3108 at 118.882 kb on + strand at 118.885 kb on + strand at 118.886 kb on - strand at 118.900 kb on + strand at 118.903 kb on - strand at 118.906 kb on + strand at 118.906 kb on + strand at 119.054 kb on - strand, within GFF3109 at 119.054 kb on - strand, within GFF3109 at 119.061 kb on + strand, within GFF3109 at 119.071 kb on + strand, within GFF3109 at 119.071 kb on + strand, within GFF3109 at 119.219 kb on - strand, within GFF3110 at 119.248 kb on + strand, within GFF3110 at 119.249 kb on - strand, within GFF3110 at 119.249 kb on - strand, within GFF3110 at 119.281 kb on + strand, within GFF3110 at 119.282 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.415 kb on + strand at 119.419 kb on + strand at 119.439 kb on - strand at 119.482 kb on + strand at 119.483 kb on - strand at 119.750 kb on - strand, within GFF3111 at 119.907 kb on - strand, within GFF3111 at 119.907 kb on - strand, within GFF3111 at 120.005 kb on - strand, within GFF3111 at 120.005 kb on - strand, within GFF3111 at 120.007 kb on + strand, within GFF3111 at 120.007 kb on + strand, within GFF3111 at 120.019 kb on + strand, within GFF3111 at 120.253 kb on + strand, within GFF3111 at 120.286 kb on + strand, within GFF3111 at 120.368 kb on + strand, within GFF3111 at 120.526 kb on + strand, within GFF3111 at 120.527 kb on - strand, within GFF3111 at 120.546 kb on + strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.547 kb on - strand, within GFF3111 at 120.767 kb on - strand, within GFF3111 at 120.938 kb on + strand at 120.939 kb on - strand at 120.974 kb on - strand at 121.014 kb on + strand at 121.015 kb on - strand at 121.017 kb on - strand at 121.078 kb on + strand at 121.180 kb on - strand at 121.182 kb on - strand at 121.182 kb on - strand at 121.183 kb on + strand at 121.183 kb on + strand at 121.184 kb on - strand at 121.291 kb on - strand at 121.497 kb on - strand, within GFF3112 at 121.692 kb on - strand, within GFF3112 at 121.876 kb on - strand at 121.880 kb on + strand at 121.881 kb on - strand at 121.938 kb on - strand at 121.939 kb on + strand at 121.939 kb on + strand at 121.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 118,512 + GFF3108 0.66 +0.4 118,513 - GFF3108 0.66 +0.3 118,588 + GFF3108 0.72 -1.2 118,588 + GFF3108 0.72 -0.3 118,641 + GFF3108 0.77 -0.2 118,882 + -0.3 118,885 + -0.8 118,886 - -1.0 118,900 + -1.2 118,903 - +0.1 118,906 + +0.1 118,906 + +0.1 119,054 - GFF3109 0.80 +0.4 119,054 - GFF3109 0.80 -0.2 119,061 + GFF3109 0.84 +0.2 119,071 + GFF3109 0.89 +0.3 119,071 + GFF3109 0.89 -0.2 119,219 - GFF3110 0.46 +1.0 119,248 + GFF3110 0.56 -0.2 119,249 - GFF3110 0.56 -0.2 119,249 - GFF3110 0.56 -0.0 119,281 + GFF3110 0.67 -0.0 119,282 - GFF3110 0.68 +0.0 119,320 - GFF3110 0.81 -0.3 119,320 - GFF3110 0.81 +0.2 119,320 - GFF3110 0.81 +0.2 119,320 - GFF3110 0.81 -0.2 119,415 + +0.3 119,419 + +0.2 119,439 - +0.0 119,482 + +1.3 119,483 - +0.4 119,750 - GFF3111 0.18 -1.9 119,907 - GFF3111 0.27 +0.3 119,907 - GFF3111 0.27 +0.6 120,005 - GFF3111 0.33 +0.2 120,005 - GFF3111 0.33 -2.7 120,007 + GFF3111 0.33 +0.1 120,007 + GFF3111 0.33 -0.8 120,019 + GFF3111 0.34 -0.4 120,253 + GFF3111 0.48 +0.0 120,286 + GFF3111 0.50 +2.0 120,368 + GFF3111 0.55 -0.4 120,526 + GFF3111 0.65 -1.3 120,527 - GFF3111 0.65 +0.1 120,546 + GFF3111 0.66 +0.8 120,547 - GFF3111 0.66 -0.4 120,547 - GFF3111 0.66 -0.2 120,547 - GFF3111 0.66 -0.9 120,767 - GFF3111 0.80 -1.4 120,938 + -0.4 120,939 - -0.0 120,974 - -0.9 121,014 + +0.3 121,015 - -1.1 121,017 - +0.1 121,078 + -0.3 121,180 - +1.4 121,182 - -0.2 121,182 - +0.1 121,183 + +0.0 121,183 + -1.8 121,184 - -0.8 121,291 - +0.3 121,497 - GFF3112 0.18 +0.6 121,692 - GFF3112 0.57 -1.1 121,876 - -0.1 121,880 + +0.6 121,881 - -0.2 121,938 - +1.9 121,939 + -0.1 121,939 + -0.6 121,984 - +0.4
Or see this region's nucleotide sequence