Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3070 and GFF3071 are separated by 272 nucleotides GFF3071 and GFF3072 are separated by 38 nucleotides GFF3072 and GFF3214 are separated by 105 nucleotides
GFF3070 - Ornithine decarboxylase (EC 4.1.1.17), at 77,908 to 80,043
GFF3070
GFF3071 - hypothetical protein, at 80,316 to 80,429
GFF3071
GFF3072 - Putative inner membrane protein YqgA, at 80,468 to 81,175
GFF3072
GFF3214 - tRNA-Phe-GAA, at 81,281 to 81,356
GFF3214
Position (kb)
80
81 Strain fitness (log2 ratio)
-2
-1
0
1 at 79.347 kb on - strand, within GFF3070 at 79.491 kb on - strand, within GFF3070 at 79.555 kb on + strand, within GFF3070 at 79.556 kb on - strand, within GFF3070 at 79.559 kb on - strand, within GFF3070 at 79.695 kb on + strand, within GFF3070 at 79.699 kb on - strand, within GFF3070 at 79.810 kb on + strand, within GFF3070 at 79.811 kb on - strand, within GFF3070 at 79.811 kb on - strand, within GFF3070 at 79.822 kb on - strand, within GFF3070 at 79.860 kb on + strand at 79.929 kb on + strand at 79.929 kb on + strand at 80.029 kb on - strand at 80.128 kb on - strand at 80.177 kb on - strand at 80.285 kb on + strand at 80.388 kb on + strand, within GFF3071 at 80.412 kb on - strand, within GFF3071 at 80.485 kb on + strand at 80.505 kb on + strand at 80.734 kb on - strand, within GFF3072 at 80.738 kb on - strand, within GFF3072 at 80.974 kb on - strand, within GFF3072 at 81.081 kb on + strand, within GFF3072 at 81.113 kb on - strand at 81.188 kb on + strand at 81.220 kb on - strand at 81.261 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 79,347 - GFF3070 0.67 +0.4 79,491 - GFF3070 0.74 +0.6 79,555 + GFF3070 0.77 -0.3 79,556 - GFF3070 0.77 +0.0 79,559 - GFF3070 0.77 -0.1 79,695 + GFF3070 0.84 -0.9 79,699 - GFF3070 0.84 +0.6 79,810 + GFF3070 0.89 +0.0 79,811 - GFF3070 0.89 +0.7 79,811 - GFF3070 0.89 -1.4 79,822 - GFF3070 0.90 -0.4 79,860 + -0.1 79,929 + -0.2 79,929 + -0.0 80,029 - -0.2 80,128 - -0.2 80,177 - +0.0 80,285 + -1.4 80,388 + GFF3071 0.63 -1.7 80,412 - GFF3071 0.84 -0.3 80,485 + -0.5 80,505 + -0.1 80,734 - GFF3072 0.38 -0.4 80,738 - GFF3072 0.38 +0.5 80,974 - GFF3072 0.71 -0.2 81,081 + GFF3072 0.87 +0.3 81,113 - +0.1 81,188 + -1.9 81,220 - -0.0 81,261 - -0.6
Or see this region's nucleotide sequence