Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3015 and GFF3016 are separated by 11 nucleotides GFF3016 and GFF3017 are separated by 2 nucleotides GFF3017 and GFF3018 are separated by 93 nucleotides GFF3018 and GFF3019 are separated by 199 nucleotides
GFF3015 - Chromosome initiation inhibitor, at 30,724 to 31,617
GFF3015
GFF3016 - FIG01047763: hypothetical protein, at 31,629 to 31,880
GFF3016
GFF3017 - Protein of unknown function DUF541, at 31,883 to 32,629
GFF3017
GFF3018 - Arginine exporter protein ArgO, at 32,723 to 33,358
GFF3018
GFF3019 - Protein involved in stability of MscS mechanosensitive channel, at 33,558 to 34,418
GFF3019
Position (kb) 31
32
33 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 31.320 kb on + strand, within GFF3015 at 31.625 kb on - strand at 31.793 kb on + strand, within GFF3016 at 31.817 kb on - strand, within GFF3016 at 31.884 kb on + strand at 32.000 kb on + strand, within GFF3017 at 32.102 kb on - strand, within GFF3017 at 32.170 kb on + strand, within GFF3017 at 32.255 kb on - strand, within GFF3017 at 32.372 kb on - strand, within GFF3017 at 32.663 kb on + strand at 32.664 kb on - strand at 32.665 kb on + strand at 32.665 kb on + strand at 32.665 kb on + strand at 32.666 kb on - strand at 32.724 kb on + strand at 32.725 kb on - strand at 32.725 kb on - strand at 32.743 kb on + strand at 32.813 kb on - strand, within GFF3018 at 32.975 kb on - strand, within GFF3018 at 32.983 kb on - strand, within GFF3018 at 32.983 kb on - strand, within GFF3018 at 33.050 kb on - strand, within GFF3018 at 33.117 kb on - strand, within GFF3018 at 33.242 kb on + strand, within GFF3018 at 33.500 kb on + strand at 33.620 kb on + strand at 33.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 31,320 + GFF3015 0.67 +0.1 31,625 - +0.4 31,793 + GFF3016 0.65 -0.1 31,817 - GFF3016 0.75 +2.3 31,884 + +0.5 32,000 + GFF3017 0.16 -0.5 32,102 - GFF3017 0.29 -1.4 32,170 + GFF3017 0.38 -0.3 32,255 - GFF3017 0.50 -0.8 32,372 - GFF3017 0.65 -0.1 32,663 + -1.6 32,664 - +0.3 32,665 + -0.4 32,665 + -0.2 32,665 + +0.1 32,666 - +1.0 32,724 + -3.9 32,725 - +0.1 32,725 - -1.7 32,743 + +0.0 32,813 - GFF3018 0.14 +0.1 32,975 - GFF3018 0.40 -0.0 32,983 - GFF3018 0.41 +0.3 32,983 - GFF3018 0.41 +0.5 33,050 - GFF3018 0.51 -0.2 33,117 - GFF3018 0.62 +0.2 33,242 + GFF3018 0.82 +0.5 33,500 + +0.2 33,620 + -0.0 33,624 - +0.0
Or see this region's nucleotide sequence