Experiment: Br60_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2104 and GFF2105 are separated by 61 nucleotides GFF2105 and GFF2106 overlap by 16 nucleotides GFF2106 and GFF2107 are separated by 424 nucleotides
GFF2104 - ATP-dependent RNA helicase SrmB, at 12,447 to 13,781
GFF2104
GFF2105 - tRNA (adenine37-N(6))-methyltransferase TrmN6 (EC 2.1.1.223), at 13,843 to 14,649
GFF2105
GFF2106 - L-aspartate oxidase (EC 1.4.3.16), at 14,634 to 16,256
GFF2106
GFF2107 - RNA polymerase sigma factor RpoE, at 16,681 to 17,256
GFF2107
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -2
-1
0
1 at 13.651 kb on - strand at 13.810 kb on + strand at 13.910 kb on - strand at 14.046 kb on - strand, within GFF2105 at 14.125 kb on - strand, within GFF2105 at 14.266 kb on - strand, within GFF2105 at 14.268 kb on + strand, within GFF2105 at 14.429 kb on + strand, within GFF2105 at 14.600 kb on - strand at 14.613 kb on + strand at 14.635 kb on + strand at 14.635 kb on + strand at 14.639 kb on + strand at 14.702 kb on - strand at 14.711 kb on + strand at 14.736 kb on - strand at 14.855 kb on + strand, within GFF2106 at 14.855 kb on + strand, within GFF2106 at 14.858 kb on + strand, within GFF2106 at 14.908 kb on + strand, within GFF2106 at 14.909 kb on - strand, within GFF2106 at 14.909 kb on - strand, within GFF2106 at 14.942 kb on + strand, within GFF2106 at 14.943 kb on - strand, within GFF2106 at 15.069 kb on + strand, within GFF2106 at 15.070 kb on - strand, within GFF2106 at 15.098 kb on + strand, within GFF2106 at 15.125 kb on - strand, within GFF2106 at 15.146 kb on - strand, within GFF2106 at 15.146 kb on - strand, within GFF2106 at 15.148 kb on - strand, within GFF2106 at 15.203 kb on - strand, within GFF2106 at 15.332 kb on + strand, within GFF2106 at 15.333 kb on - strand, within GFF2106 at 15.333 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.450 kb on - strand, within GFF2106 at 15.454 kb on + strand, within GFF2106 at 15.617 kb on + strand, within GFF2106 at 15.626 kb on - strand, within GFF2106 at 15.762 kb on + strand, within GFF2106 at 15.910 kb on + strand, within GFF2106 at 15.911 kb on - strand, within GFF2106 at 15.911 kb on - strand, within GFF2106 at 15.911 kb on - strand, within GFF2106 at 15.911 kb on - strand, within GFF2106 at 16.050 kb on - strand, within GFF2106 at 16.050 kb on - strand, within GFF2106 at 16.114 kb on + strand at 16.115 kb on - strand at 16.164 kb on - strand at 16.164 kb on - strand at 16.303 kb on + strand at 16.303 kb on + strand at 16.303 kb on + strand at 16.304 kb on - strand at 16.304 kb on - strand at 16.304 kb on - strand at 16.304 kb on - strand at 16.304 kb on - strand at 16.324 kb on - strand at 16.516 kb on - strand at 16.518 kb on + strand at 16.614 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Br60_phage 15 MOI remove 13,651 - -0.1 13,810 + -0.2 13,910 - -0.4 14,046 - GFF2105 0.25 -0.2 14,125 - GFF2105 0.35 -0.2 14,266 - GFF2105 0.52 -2.0 14,268 + GFF2105 0.53 -0.0 14,429 + GFF2105 0.73 +1.0 14,600 - -0.6 14,613 + +0.9 14,635 + -0.4 14,635 + -0.5 14,639 + -0.1 14,702 - -0.5 14,711 + +0.5 14,736 - -1.3 14,855 + GFF2106 0.14 +0.4 14,855 + GFF2106 0.14 -1.5 14,858 + GFF2106 0.14 -2.1 14,908 + GFF2106 0.17 -0.2 14,909 - GFF2106 0.17 -0.1 14,909 - GFF2106 0.17 -0.3 14,942 + GFF2106 0.19 +0.6 14,943 - GFF2106 0.19 +0.0 15,069 + GFF2106 0.27 -0.2 15,070 - GFF2106 0.27 -0.2 15,098 + GFF2106 0.29 -2.1 15,125 - GFF2106 0.30 +1.4 15,146 - GFF2106 0.32 -0.1 15,146 - GFF2106 0.32 -0.3 15,148 - GFF2106 0.32 -0.9 15,203 - GFF2106 0.35 -0.3 15,332 + GFF2106 0.43 +1.2 15,333 - GFF2106 0.43 -0.4 15,333 - GFF2106 0.43 -0.1 15,450 - GFF2106 0.50 +0.7 15,450 - GFF2106 0.50 -0.1 15,450 - GFF2106 0.50 -0.7 15,454 + GFF2106 0.51 -0.1 15,617 + GFF2106 0.61 +0.2 15,626 - GFF2106 0.61 -0.2 15,762 + GFF2106 0.70 +0.8 15,910 + GFF2106 0.79 -0.9 15,911 - GFF2106 0.79 -0.7 15,911 - GFF2106 0.79 -0.3 15,911 - GFF2106 0.79 -0.6 15,911 - GFF2106 0.79 +0.1 16,050 - GFF2106 0.87 +0.4 16,050 - GFF2106 0.87 -0.3 16,114 + -0.3 16,115 - +0.5 16,164 - -0.5 16,164 - -1.4 16,303 + -1.6 16,303 + +0.7 16,303 + -1.1 16,304 - +0.0 16,304 - -0.1 16,304 - -0.2 16,304 - +0.9 16,304 - -0.5 16,324 - -0.0 16,516 - +1.4 16,518 + +0.3 16,614 + -0.3
Or see this region's nucleotide sequence