Experiment: 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF405 and GFF406 are separated by 267 nucleotides GFF406 and GFF407 overlap by 20 nucleotides GFF407 and GFF408 overlap by 33 nucleotides GFF408 and GFF409 are separated by 63 nucleotides
GFF405 - L-talarate dehydratase @ Galactarate dehydratase (EC 4.2.1.42), at 120,427 to 121,623
GFF405
GFF406 - Transcriptional regulator, LacI family, at 121,891 to 122,946
GFF406
GFF407 - hypothetical protein, at 122,927 to 123,046
GFF407
GFF408 - 'tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207)' transl_table=11, at 123,014 to 123,487
GFF408
GFF409 - L-lactate dehydrogenase (EC 1.1.2.3), at 123,551 to 124,741
GFF409
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-2
-1
0
1 at 121.005 kb on + strand, within GFF405 at 121.104 kb on + strand, within GFF405 at 121.192 kb on - strand, within GFF405 at 121.230 kb on + strand, within GFF405 at 121.231 kb on - strand, within GFF405 at 121.291 kb on + strand, within GFF405 at 121.325 kb on - strand, within GFF405 at 121.337 kb on - strand, within GFF405 at 121.355 kb on + strand, within GFF405 at 121.356 kb on - strand, within GFF405 at 121.356 kb on - strand, within GFF405 at 121.360 kb on - strand, within GFF405 at 121.386 kb on - strand, within GFF405 at 121.478 kb on - strand, within GFF405 at 121.525 kb on + strand at 121.573 kb on - strand at 121.589 kb on + strand at 121.615 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.725 kb on + strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.726 kb on - strand at 121.735 kb on - strand at 121.848 kb on - strand at 121.880 kb on + strand at 121.881 kb on - strand at 122.099 kb on - strand, within GFF406 at 122.148 kb on - strand, within GFF406 at 122.241 kb on - strand, within GFF406 at 122.260 kb on + strand, within GFF406 at 122.348 kb on + strand, within GFF406 at 122.473 kb on + strand, within GFF406 at 122.474 kb on - strand, within GFF406 at 122.553 kb on - strand, within GFF406 at 122.553 kb on - strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.556 kb on + strand, within GFF406 at 122.557 kb on - strand, within GFF406 at 122.811 kb on + strand, within GFF406 at 122.814 kb on + strand, within GFF406 at 123.009 kb on - strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.010 kb on + strand, within GFF407 at 123.011 kb on - strand, within GFF407 at 123.064 kb on - strand, within GFF408 at 123.142 kb on + strand, within GFF408 at 123.216 kb on + strand, within GFF408 at 123.216 kb on + strand, within GFF408 at 123.217 kb on - strand, within GFF408 at 123.301 kb on - strand, within GFF408 at 123.377 kb on - strand, within GFF408 at 123.473 kb on + strand at 123.474 kb on - strand at 123.748 kb on - strand, within GFF409
Per-strain Table
Position Strand Gene LocusTag Fraction 6F2_phage 15 MOI remove 121,005 + GFF405 0.48 +0.1 121,104 + GFF405 0.57 -0.1 121,192 - GFF405 0.64 +0.2 121,230 + GFF405 0.67 +0.2 121,231 - GFF405 0.67 -0.1 121,291 + GFF405 0.72 -0.1 121,325 - GFF405 0.75 +1.2 121,337 - GFF405 0.76 -1.6 121,355 + GFF405 0.78 +0.7 121,356 - GFF405 0.78 +0.1 121,356 - GFF405 0.78 +0.6 121,360 - GFF405 0.78 -0.0 121,386 - GFF405 0.80 -0.1 121,478 - GFF405 0.88 +0.3 121,525 + +1.1 121,573 - +0.6 121,589 + -0.1 121,615 + -0.1 121,725 + +0.2 121,725 + -0.4 121,725 + -0.5 121,725 + +0.7 121,725 + -0.5 121,725 + +0.1 121,725 + -0.1 121,726 - -0.5 121,726 - -0.2 121,726 - -0.3 121,726 - +0.3 121,726 - +0.5 121,726 - -0.0 121,726 - -0.0 121,735 - +0.3 121,848 - -0.0 121,880 + -0.4 121,881 - +0.1 122,099 - GFF406 0.20 +0.3 122,148 - GFF406 0.24 -0.1 122,241 - GFF406 0.33 +0.2 122,260 + GFF406 0.35 -0.4 122,348 + GFF406 0.43 +0.3 122,473 + GFF406 0.55 +0.9 122,474 - GFF406 0.55 -0.1 122,553 - GFF406 0.63 +0.6 122,553 - GFF406 0.63 +0.6 122,556 + GFF406 0.63 +0.5 122,556 + GFF406 0.63 -0.3 122,557 - GFF406 0.63 -0.2 122,811 + GFF406 0.87 -0.4 122,814 + GFF406 0.87 -1.9 123,009 - GFF407 0.68 -1.9 123,010 + GFF407 0.69 +1.3 123,010 + GFF407 0.69 +0.3 123,010 + GFF407 0.69 -0.9 123,011 - GFF407 0.70 -0.8 123,064 - GFF408 0.11 +0.4 123,142 + GFF408 0.27 -0.2 123,216 + GFF408 0.43 -0.5 123,216 + GFF408 0.43 +1.5 123,217 - GFF408 0.43 -0.2 123,301 - GFF408 0.61 +0.1 123,377 - GFF408 0.77 +1.1 123,473 + +0.2 123,474 - -0.3 123,748 - GFF409 0.17 +1.1
Or see this region's nucleotide sequence